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Offlinefractaldill
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Any probems with using SFD's for liquid cultures?
    #19363674 - 01/03/14 02:31 PM (10 years, 27 days ago)

Want to be professional, but dont want to dish out the money for syringe filters.


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OfflinecronicrFacebook
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Re: Any probems with using SFD's for liquid cultures? [Re: fractaldill]
    #19363698 - 01/03/14 02:37 PM (10 years, 27 days ago)

you risk contams if the filter gets wet


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Offlinefractaldill
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Re: Any probems with using SFD's for liquid cultures? [Re: cronicr]
    #19363710 - 01/03/14 02:41 PM (10 years, 27 days ago)

exactly what i was worrying about. but how exactly would that risk contamination? unless it grew through the filter.


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OfflinecronicrFacebook
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Re: Any probems with using SFD's for liquid cultures? [Re: fractaldill]
    #19363729 - 01/03/14 02:46 PM (10 years, 27 days ago)

thats exactly what happens, just wicks them in:thumbup:


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Offlineinvitro


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Re: Any probems with using SFD's for liquid cultures? [Re: cronicr]
    #19363742 - 01/03/14 02:48 PM (10 years, 27 days ago)

Is that also a concern with syringe filters?


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OfflinecronicrFacebook
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Re: Any probems with using SFD's for liquid cultures? [Re: invitro]
    #19363761 - 01/03/14 02:51 PM (10 years, 27 days ago)

nope, thats y there the best suited filter for lc's:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineinvitro


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Re: Any probems with using SFD's for liquid cultures? [Re: cronicr]
    #19363919 - 01/03/14 03:28 PM (10 years, 27 days ago)

The sfds are made from polypropylene as far as I know

and so are syringe filters. see:
http://www.sigmaaldrich.com/labware/labware-products.html?TablePage=20442122

How do the syringe filters not have the problem of wicking contams then?  If you have a lc with a syringe filter and turn it upside down, will any water leak through?


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Re: Any probems with using SFD's for liquid cultures? [Re: invitro]
    #19363947 - 01/03/14 03:33 PM (10 years, 27 days ago)

a whatman will ensure no liquid touches your fuliters, check tl's guide out:thumbup:
lc's don't require that much ge, just enough to ensure theres no vaccuum created


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I'm tired do me a favor


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Offlineinvitro


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Re: Any probems with using SFD's for liquid cultures? [Re: cronicr]
    #19364054 - 01/03/14 04:00 PM (10 years, 27 days ago)

Ok I get it now.  Suppose I make a lc jar without a gas exchange filter of any sort.  How long do you suppose the mycelium will last?
I actually want to do this because I want to use it all up fairly quickly (2 weeks) and not store it.


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Offlinemonoculture
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Re: Any probems with using SFD's for liquid cultures? [Re: invitro]
    #19364132 - 01/03/14 04:18 PM (10 years, 27 days ago)

You don't need any for a LC, as long as you leave enough air in the jar, so only fill it for 2/3.
I'm having LC that has been in the jar for 2 months which is still fine, so your two weeks won't matter.
When you do still want syringe filters, I have some laying around I tried to use with jars, but replaced them with felt.

When using jars without syringe filters, when taking out LC there will be a negative pressure. So you need to inject a full syringe of air before you take one out. So a syringe filter in the jar lid can also be handy to not need that step.
I make syringes filled with clean air in front of my flow-hood, but you could also wrap a syringe in foil and PC it I guess.
You also might get yourself a few syringe filters and needles, which you PC connected to each other wrapped in foil to use to stabilize pressure in the jar to the outside.


Edited by monoculture (01/03/14 04:24 PM)


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