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InvisibleMudaFuka
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Re: Next logical step after PF [Re: cronicr]
    #19363209 - 01/03/14 12:30 PM (10 years, 1 month ago)

GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.


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Re: Next logical step after PF [Re: MudaFuka]
    #19363218 - 01/03/14 12:32 PM (10 years, 1 month ago)

Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.



:whathesaid:


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It doesn't matter what i think of you...all that matters is clean spawn

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Re: Next logical step after PF [Re: MudaFuka]
    #19363282 - 01/03/14 12:48 PM (10 years, 1 month ago)

Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams


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InvisibleMudaFuka
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Re: Next logical step after PF [Re: loki44]
    #19363303 - 01/03/14 12:56 PM (10 years, 1 month ago)

Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



whatever works for you man. Different strokes for different folks.


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Re: Next logical step after PF [Re: loki44]
    #19363349 - 01/03/14 01:08 PM (10 years, 1 month ago)

Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:


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InvisibleMudaFuka
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Re: Next logical step after PF [Re: cronicr]
    #19363372 - 01/03/14 01:11 PM (10 years, 1 month ago)

:yeahthatsright:


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Offlineloki44
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Re: Next logical step after PF [Re: cronicr]
    #19363386 - 01/03/14 01:14 PM (10 years, 1 month ago)

Quote:

cronicr said:
Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:




2ccs on 1/3 of a half pint jar is not much grain for the mycelium to finish, 2 weeks maybe and after that you are ready to incoc as many jars as you can because that wild yield you 500 cc of mycelium water

no agar, no petris, no transfers,no senescence, no g2g, just "plug and play" i.e. inject ans squeeze :syringe: :tongue2:


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Re: Next logical step after PF [Re: loki44]
    #19363393 - 01/03/14 01:15 PM (10 years, 1 month ago)

Quote:

loki44 said:
Quote:

cronicr said:
Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:




2ccs on 1/3 of a half pint jar is not much grain for the mycelium to finish, 2 weeks maybe and after that you are ready to incoc as many jars as you can because that wild yield you 500 cc of mycelium water

no agar, no petris, no transfers,no senescence, no g2g, just "plug and play" i.e. inject ans squeeze :syringe: :tongue2:



:rockon:
i'll always prefer agar but:rockon:
try start your lc from a wedge to a real lc medium, i find it stores better
and by your logic his ms to grain is still faster lol


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Edited by cronicr (01/03/14 01:16 PM)


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Offlineloki44
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Re: Next logical step after PF [Re: cronicr]
    #19363442 - 01/03/14 01:26 PM (10 years, 1 month ago)

and you can still can get piggy backed contams on your agar and transfer to your grains so at the end of the day there's always a risk, lets not pretend that agar will never get you contams, its better than GLC but not by much


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Re: Next logical step after PF [Re: loki44]
    #19363449 - 01/03/14 01:27 PM (10 years, 1 month ago)

your really gonna bring up contams as an arguement here...:rolleyes:


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Re: Next logical step after PF [Re: cronicr]
    #19363465 - 01/03/14 01:32 PM (10 years, 1 month ago)

hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:


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Re: Next logical step after PF [Re: loki44]
    #19363466 - 01/03/14 01:33 PM (10 years, 1 month ago)

There are simple ways to avoid piggyback contams on agar, just most people are either to lazy to do it or just don't know how. Conversely GLC is super risky IMO, just cause a jar looks clean does not mean it is. There is a reason we smell jars before spawning or after G2G. Even then it is very possible for contams like mold which will not be easy to smell through your filter to be hiding in the centre.

Anyone who has run over 100 quarts knows what I'm talking about.


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Re: Next logical step after PF [Re: loki44]
    #19363478 - 01/03/14 01:36 PM (10 years, 1 month ago)

Quote:

loki44 said:
hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:



the needle is only half the issue, who's to say your ms inoculated master is 100% clean:smirk:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363485 - 01/03/14 01:37 PM (10 years, 1 month ago)

ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?


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Re: Next logical step after PF [Re: loki44]
    #19363488 - 01/03/14 01:38 PM (10 years, 1 month ago)

Quote:

loki44 said:
hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:



Most contams that spread during SAB work do so through contact. A syringe needle makes contact with the inside of the jar via the SHIP, your scalpel does not. But what do I know, I only do 30-40 quarts a month :shrug:


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Re: Next logical step after PF [Re: loki44]
    #19363502 - 01/03/14 01:40 PM (10 years, 1 month ago)

Quote:

loki44 said:
ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?



loki your a one man army here lol, i like lc's but your arguing for nuthing,had you started from a wedge for your original jar and not tried to use faulty logic i would have said.....
:manofapproval:
but like i said faulty logic isn't gonna win ya this one:shocked:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363514 - 01/03/14 01:43 PM (10 years, 1 month ago)

ok, so where do you think the contams are coming from, because you are not using inoc, just agar wedges and g2g right?


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InvisiblePastywhyteMDiscord
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Re: Next logical step after PF [Re: loki44]
    #19363523 - 01/03/14 01:45 PM (10 years, 1 month ago)

Quote:

loki44 said:
ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?



Not sure what your referring to. My last contam was after the third flush, which I was tempted to blame on my spawn. But after some consideration I decided if my spawn was to blame, that my inoculate was clean and it was my filter that let me down. I have now switched all my filters to SFD and it seems to have corrected the issue. However using an ms syringe is often a risky way to inoculate,  and to make a LC from one be it a GLC or LC from spores, you still run the risk of the contams from the original ms syringe contaming your culture.


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363529 - 01/03/14 01:47 PM (10 years, 1 month ago)

:whathesaid:
i'm not saying your method doesn't work so chill out, just stop saying that it's better then agar because you have no argument there lol


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363530 - 01/03/14 01:47 PM (10 years, 1 month ago)

Also I have used ms syringes to nock up jars, I just dont do it much anymore.


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