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RandomFX
protege


Registered: 12/02/13
Posts: 1,015
Loc: North-East, USA
Last seen: 5 years, 1 month
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Should I do a spore print on first grow? 1
#19363182 - 01/03/14 12:23 PM (10 years, 28 days ago) |
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By now everyone knows I am a newb, but I am right now fruiting my first grey oyster shrooms, they are not super duper producers, but I have a few and they are starting to get respectfully big imo. I am not a professional grower obviously, so am not looking to produce some super strain or anything, although of course would like to have good success, and these were grown from some plugs I got for cheap and used the plugs to inoculate brf cakes and then substrate. Anyways, I'd like opinions. Should I take a large shroom that is growing and make a spore print from it, to make a liquid culture to continue having stuff to work with, or should I just buy a new liquid culture?, Should I cut the inside of part of the largest growing one to grow/clone mycelium from it?
I haven't gotten agar yet, though I plan to, and there has been someone here who has been so much of a help, and even offered to send me some agar and plug spawn, for no reason than to be awesome, (I am really impressed by the quality of character in this forum. They even told me not to worry about the shipping cost, which I of course expected to pay, a favor I expect to return either to them one day or someone else on this forum to continue the good will.)
but, the real question is I guess, is what is the best way to proceed? My gardener side of me, says, the spore print like seeds 'may' be untrust worthy, like seeds from a plant or children from people, you would surely have some weak ones and some strong ones, right? when you really just want the strong ones? however if you use the material from the shroom itself, it is a clone right, so you will never get a stronger one...though you won't get a weaker one either right? or am I wrong on this line of thinking? anyways, I know there are plenty of people here who know what they are doing, so thus I ask for input to save myself all the work of finding out myself and wasting time.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Should I do a spore print on first grow? [Re: RandomFX] 1
#19363211 - 01/03/14 12:30 PM (10 years, 28 days ago) |
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If you were to move from a print you would want to go with agar then LC.
So, wait for the agar and start that up. Always a good idea. You will run into many bumps in the road to begin with but that is ok. You gotta do it sometime.
If you are looking for mushrooms sooner than later and that is all I would stick to getting a culture instead of going from print.
But by all means go for the print start if you want the experience.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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RandomFX
protege


Registered: 12/02/13
Posts: 1,015
Loc: North-East, USA
Last seen: 5 years, 1 month
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Re: Should I do a spore print on first grow? [Re: Ganzig]
#19363505 - 01/03/14 01:41 PM (10 years, 27 days ago) |
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ok, so what is the opinion on going with a print, as opposed to cutting some from the inside and going with a clone? I have never done either yet, so do not know which is better, or which makes more sense or not.
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ThatsWhatYouGet

Registered: 04/22/12
Posts: 33
Loc: Nebraska
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Re: Should I do a spore print on first grow? [Re: RandomFX]
#19363535 - 01/03/14 01:49 PM (10 years, 27 days ago) |
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The clone would be faster and more noob friendly for sure. A print is going to take much more time and materials.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Should I do a spore print on first grow? [Re: ThatsWhatYouGet]
#19363551 - 01/03/14 01:54 PM (10 years, 27 days ago) |
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Quote:
ThatsWhatYouGet said: The clone would be faster and more noob friendly for sure. A print is going to take much more time and materials.
QFT
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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FoxFire
Energized Matter



Registered: 07/17/05
Posts: 1,281
Loc: PNW
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Re: Should I do a spore print on first grow? [Re: RandomFX]
#19363566 - 01/03/14 01:57 PM (10 years, 27 days ago) |
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If its a poor strain though, I wouldn't clone it, that's for sure.
If you take a spore print, that's fine, but then you would have to do a lot of strain isolation on agar plates. That means fruiting them all out to see which one is fastest/best producer/grows on preferred substrate etc.
Then once you have a good culture, you can store it long term in the fridge in test tube slants.
I still take prints from time to time just for fun, but it's not that practical when a brand new syringe only costs 20 bucks.
-FF
-------------------- Unless someone like you cares a whole awful lot, Nothing is going to get better. It's not.
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ThatsWhatYouGet

Registered: 04/22/12
Posts: 33
Loc: Nebraska
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Re: Should I do a spore print on first grow? [Re: FoxFire]
#19364027 - 01/03/14 03:49 PM (10 years, 27 days ago) |
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If it were me I would clone my current fruits and take spore prints. This will give you plenty of opportunities to practice your agar prep and sterile technique.
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RandomFX
protege


Registered: 12/02/13
Posts: 1,015
Loc: North-East, USA
Last seen: 5 years, 1 month
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Re: Should I do a spore print on first grow? [Re: ThatsWhatYouGet]
#19364409 - 01/03/14 05:07 PM (10 years, 27 days ago) |
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thanks for the wisdom. it all makes sense. guess tomorrow I'll order me some mae.
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