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OfflineFractal420
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Next logical step after PF
    #19360822 - 01/02/14 09:28 PM (10 years, 28 days ago)

After 2 successful PF jar projects, I was curious about what the next step in learning about mycology and improving practices should be, and after some reading and thought, it seems to be either casing with verm, using wbs pints, or spawn bags. I'm personally very interested in less obvious methods, and things like the mad bag appeal to me. But I seem to hear mixed things about in vitro. It seems like a great way to keep the project minimal which is ideal to my situation, but how well do "mad bags" do compared to PF jars for example? Do they really provide a good environment on their own or should one keep it in their SGFC with perlite? Do they do better if open or used as spawn?

Just trying to advance my mycological methods, and wondering the best way to go forward and whether in vitro is worth a shot or whether straight to casings/bulk. You guys taught me everything I needed thus far :smile:

I was planning on using a PE LC to try out the bag tek, but I hear spores work well too


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Re: Next logical step after PF [Re: Fractal420]
    #19360852 - 01/02/14 09:33 PM (10 years, 28 days ago)

If a method of growth interests you give it a shot. The more you try the more you will learn.


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Re: Next logical step after PF [Re: MudaFuka]
    #19360871 - 01/02/14 09:38 PM (10 years, 28 days ago)

Agar with strain isolation to wild bird seed spawned to coconut coir and vermiculite in a monotub cased with your choice of casing material.

EDIT: Also the monotub thing doesn't have to be a 'full size' monotub. You can isolate a strain on no pour agar cooked in a food storage container then isolate a strain and colonize one or two jars or one mad bag.  I wouldn't fruit in the bag. I would spawn it to a minimontub.


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Edited by elasticaltiger (01/02/14 09:40 PM)


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Re: Next logical step after PF [Re: elasticaltiger]
    #19360912 - 01/02/14 09:47 PM (10 years, 28 days ago)

if you are going to do agar check out Pastywhite's agar tek.


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OfflineFractal420
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Re: Next logical step after PF [Re: MudaFuka]
    #19361199 - 01/02/14 10:55 PM (10 years, 28 days ago)

I want to explore something a bit stealthy which is why the self-contained "mad bag" has me interested. Also, cakes can take a long time to fruit, with the last ones flushing in the double digits, which is why I thought of doing a casing if not a spawn bag


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Re: Next logical step after PF [Re: Fractal420]
    #19361212 - 01/02/14 10:58 PM (10 years, 28 days ago)

when you say casing do you mean spawning to bulk and then adding a casing layer?


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Re: Next logical step after PF [Re: MudaFuka]
    #19361235 - 01/02/14 11:03 PM (10 years, 28 days ago)

Quote:

Fractal420 said:
I want to explore something a bit stealthy which is why the self-contained "mad bag" has me interested. Also, cakes can take a long time to fruit, with the last ones flushing in the double digits, which is why I thought of doing a casing if not a spawn bag




You will still need a light, and fruiting invitro usually gives poor results. Stealth and growing mushrooms don't really go that well together. If you really need stealth look into sclerotia.

Quote:

elasticaltiger said:
EDIT: Also the monotub thing doesn't have to be a 'full size' monotub. You can isolate a strain on no pour agar cooked in a food storage container then isolate a strain and colonize one or two jars or one mad bag.  I wouldn't fruit in the bag. I would spawn it to a minimontub.



^^^This. I have a bunch of 20 quart monotubs and they are great. Could fit under a bed or into a closet easily, really they won't take up much more room than your bag, but you will see better results in the end. This was 1.5 quarts of spawn and 2 quarts sub.



Quote:

MudaFuka said:
if you are going to do agar check out Pastywhite's agar tek.



Its as easy as pf tek for sure :thumbup:


Edited by Pastywhyte (01/02/14 11:16 PM)


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Re: Next logical step after PF [Re: Pastywhyte]
    #19361262 - 01/02/14 11:10 PM (10 years, 28 days ago)

sorry I spelled your name wrong Pasty.


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Re: Next logical step after PF [Re: MudaFuka]
    #19361269 - 01/02/14 11:13 PM (10 years, 28 days ago)

Quote:

MudaFuka said:
sorry I spelled your name wrong Pasty.



:lmafo: Lots of people do :lol:


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Re: Next logical step after PF [Re: Pastywhyte]
    #19361324 - 01/02/14 11:28 PM (10 years, 28 days ago)

Pasty when you say 1.5 quarts of spawn do you mean 3 jars of grain 1/2 full? or two jars 2/3 full? or 1 and 1/2 'regular amount of grain in a spawn jar' jars. lol does that make sense?  Are you trying to accurately measure the amount of grain or is it 1 and 1/2 jars worth where each jar is not completely full?


--------------------
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No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

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Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (01/02/14 11:35 PM)


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Re: Next logical step after PF [Re: elasticaltiger]
    #19361328 - 01/02/14 11:30 PM (10 years, 28 days ago)

Naw, I usually fill my quarts about 2/3 to 3/4 full. When I did that tub I did another one just like it and took 3 quarts that were 2/3 to 3/4 full and split them between the two tubs. So in essence 1.5 quarts was probably like 1.2 quarts or so.


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Re: Next logical step after PF [Re: Pastywhyte]
    #19361346 - 01/02/14 11:34 PM (10 years, 28 days ago)

Quote:

Pastywhyte said:
Naw, I usually fill my quarts about 2/3 to 3/4 full. When I did that tub I did another one just like it and took 3 quarts that were 2/3 to 3/4 full and split them between the two tubs. So in essence 1.5 quarts was probably like 1.2 quarts or so.




I am so happy you understood what I was trying to ask. This was the answer I was looking for. Thank you. :takingnotes:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineFractal420
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Re: Next logical step after PF [Re: MudaFuka]
    #19362666 - 01/03/14 10:08 AM (10 years, 27 days ago)

Quote:

MudaFuka said:
when you say casing do you mean spawning to bulk and then adding a casing layer?




Yes, it seems the ways to go forward are either to use spawn/grain and using a tray with 50/50. Some people seem to progress by breaking up their PF jars and casing those if I'm not mistaken (once they are fully colonized), some switch to WBS or straw, but it seems the casing layer is an important step, and so far I'm only really familiar with the SGFC and cakes. I've become pretty good at rolling, dunking, setting all that stuff up.

Oh, and I don't mind the 6500k light, I can set that up in a closet on a timer. If in vitro isn't a good option I won't really use it, or at least not without the knowledge to open and fruit it using casing if its not working out. What happens if one leaves spawn bags in the FC? They would have the humidity they need, but is the bag climate not good enough? I've heard some noob talk (I'm a noob too I guess) about "2 ounces per mad bag" which seems decent if using a lot of them (I only state the two ounce thing as an example, I know how variable it can actually be). The thing that got me interested in the bags is they seem to have a lot of substrate at once, pre-sterilized, cheap, and seems to have potential. However like I said, I'm just trying to do whatever wouldn't be too out of reach from PF jars, but something that would still teach me. I'm starting to think monotub.

I've been reading some info on the subject and finished that "let's grow mushrooms" video, right after PF it seems to jump into casing layers, and then straw.

Question: why agar strain isolation? I don't really mind MS syringes from our friends, have worked out very well in the past, and I managed one LC from PE (original...as in not PE#6)


Edited by Fractal420 (01/03/14 10:14 AM)


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Re: Next logical step after PF [Re: Fractal420]
    #19362713 - 01/03/14 10:21 AM (10 years, 27 days ago)

If you are growing cubs a casing layer isn't necessary, though many people use one with success.
You can yield more using WBS than crumbled cakes for spawn. Grains are also great because they let you do G2G transfers giving you the ability to make as much spawn as you can handle.
I would spawn to an 80:20 coir verm sub with just a hand full of gypsum.Use a spawn to sub ratio between 1:2 and 1:4.
A mono tub is likely your best option for an FC. putting smaller tubs in a SGFC is an option but the tubs can sometimes restrict air flow within the SGFC.
Agar is better than MS because it gives consistent growth. When you use spores you are working with potentially millions of different genetics.


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Edited by MudaFuka (01/03/14 10:24 AM)


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OfflineFractal420
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Re: Next logical step after PF [Re: MudaFuka]
    #19362869 - 01/03/14 11:03 AM (10 years, 27 days ago)

I also just saw this pic, (it's NOT my pic, I was surfing other entries) and found this interesting:



So, like this person, I'm curious, why not just put a bunch of trays in the shotgun? Seems to be working well and probably not that much harder than cakes in a shotgun. It also seems like it would all grow at once instead of constantly harvesting cakes (the only problem with this is the time it takes sometimes, I spent 3 months babysitting my BRF cakes last time as they flushed and flushed, and this isn't a problem, but quicker is better to me... I am speaking about just fruiting, I don't mind waiting for colonization, but when fruiting can't really go out for a weekend, etc).

What would be the downside of this tray in SG thing vs monotub? I already have a very nice SG ready, and I'm not opposed to making a monotub, I'm just wondering what's the difference between these two teks? I know Mudafucka said it could reduce air flow?


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Re: Next logical step after PF [Re: Fractal420]
    #19362943 - 01/03/14 11:23 AM (10 years, 27 days ago)

I'll also say agar (or agar like mediums). What you inoculate with the agar is up to you but it is certainly a large step in studying mycology.


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Re: Next logical step after PF [Re: Fractal420]
    #19362989 - 01/03/14 11:34 AM (10 years, 27 days ago)

Quote:

Fractal420 said:
I also just saw this pic, (it's NOT my pic, I was surfing other entries) and found this interesting:



So, like this person, I'm curious, why not just put a bunch of trays in the shotgun? Seems to be working well and probably not that much harder than cakes in a shotgun. It also seems like it would all grow at once instead of constantly harvesting cakes (the only problem with this is the time it takes sometimes, I spent 3 months babysitting my BRF cakes last time as they flushed and flushed, and this isn't a problem, but quicker is better to me... I am speaking about just fruiting, I don't mind waiting for colonization, but when fruiting can't really go out for a weekend, etc).

What would be the downside of this tray in SG thing vs monotub? I already have a very nice SG ready, and I'm not opposed to making a monotub, I'm just wondering what's the difference between these two teks? I know Mudafucka said it could reduce air flow?



small trays like that are fine in a SGFC its just when they get bigger that they become a problem.


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Re: Next logical step after PF [Re: Fractal420]
    #19363043 - 01/03/14 11:45 AM (10 years, 27 days ago)

OP I was in the same situation as you, did 2 successful PF-tek grows and what I did is just jumped into monotubs

got myself:

-2 dozen quart jars
-SFDs + vacutainers and rtv silicone(for the lids)
-rye berries from health food store
-prepared a GLC and inoculate on SAB

end up with 23 quart jars of grain spawn

then get coir from the pet store and verm for your bulk substrate, hydrate to field capacity, pasteurize and then mix it with you fully colonized grain spawn, bang got 3 monotubs going

it may look like is more difficult than pf-tek but is not , just a couple steps more here and there

dont listen to people trying to get you into agar isolates, that will really complicate the issue because you need not only to prepare a shitload of petris but also you got to test which ones are good fruiters/good potency on cakes

no wonder new growers get discouraged to jump into bulk grows

just go with GLC and MS inoculations and you will be fine


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Re: Next logical step after PF [Re: loki44]
    #19363140 - 01/03/14 12:13 PM (10 years, 27 days ago)

Quote:

loki44 said:
OP I was in the same situation as you, did 2 successful PF-tek grows and what I did is just jumped into monotubs

got myself:

-2 dozen quart jars
-SFDs + vacutainers and rtv silicone(for the lids)
-rye berries from health food store
-prepared a GLC and inoculate on SAB

end up with 23 quart jars of grain spawn

then get coir from the pet store and verm for your bulk substrate, hydrate to field capacity, pasteurize and then mix it with you fully colonized grain spawn, bang got 3 monotubs going

it may look like is more difficult than pf-tek but is not , just a couple steps more here and there

dont listen to people trying to get you into agar isolates, that will really complicate the issue because you need not only to prepare a shitload of petris but also you got to test which ones are good fruiters/good potency on cakes

no wonder new growers get discouraged to jump into bulk grows

just go with GLC and MS inoculations and you will be fine




I like this advice and I see no real problem in using syringes, seems the only thing that changes with agar is genetics. Can someone clear this up if there is any real advantage besides simply isolating one race of cube? To me cubes are cubes as long as they're taken care of well and come from a good place. Also what's a GLC, I don't recognize the acronym but I know what a liquid culture is. Can't one simply just use MS syringes? What's the issue with that if there is one?


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Re: Next logical step after PF [Re: Fractal420]
    #19363169 - 01/03/14 12:20 PM (10 years, 27 days ago)

clean cultures,better storage,awesome expanding,cheap,easy,shroomery approved...agar in a nutshell:rockon:


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Re: Next logical step after PF [Re: cronicr]
    #19363209 - 01/03/14 12:30 PM (10 years, 27 days ago)

GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.


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Re: Next logical step after PF [Re: MudaFuka]
    #19363218 - 01/03/14 12:32 PM (10 years, 27 days ago)

Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.



:whathesaid:


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Re: Next logical step after PF [Re: MudaFuka]
    #19363282 - 01/03/14 12:48 PM (10 years, 27 days ago)

Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams


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Re: Next logical step after PF [Re: loki44]
    #19363303 - 01/03/14 12:56 PM (10 years, 27 days ago)

Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



whatever works for you man. Different strokes for different folks.


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Re: Next logical step after PF [Re: loki44]
    #19363349 - 01/03/14 01:08 PM (10 years, 27 days ago)

Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:


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Re: Next logical step after PF [Re: cronicr]
    #19363372 - 01/03/14 01:11 PM (10 years, 27 days ago)

:yeahthatsright:


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Re: Next logical step after PF [Re: cronicr]
    #19363386 - 01/03/14 01:14 PM (10 years, 27 days ago)

Quote:

cronicr said:
Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:




2ccs on 1/3 of a half pint jar is not much grain for the mycelium to finish, 2 weeks maybe and after that you are ready to incoc as many jars as you can because that wild yield you 500 cc of mycelium water

no agar, no petris, no transfers,no senescence, no g2g, just "plug and play" i.e. inject ans squeeze :syringe: :tongue2:


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Re: Next logical step after PF [Re: loki44]
    #19363393 - 01/03/14 01:15 PM (10 years, 27 days ago)

Quote:

loki44 said:
Quote:

cronicr said:
Quote:

loki44 said:
Quote:

MudaFuka said:
GLC=grain liquid culture. I wouldn't bother doing a liquid culture without first using agar to make sure its not contaminated.
Just nock up your jars with MS. You could always play around with some agar at the same time. It will give you something to do while you wait for things to colonize.





1cc/quart jar of MS will take weeks to colonize, 15cc/quart jar of GLC will finish the jar in 5/6 days

also there is no way to know if the MS syringe is clean, with GLC you can visually inspect the mycelium in your  grain jar to check for any contams



yeh but your not counting the time it took to colonize the original jar:smirk:




2ccs on 1/3 of a half pint jar is not much grain for the mycelium to finish, 2 weeks maybe and after that you are ready to incoc as many jars as you can because that wild yield you 500 cc of mycelium water

no agar, no petris, no transfers,no senescence, no g2g, just "plug and play" i.e. inject ans squeeze :syringe: :tongue2:



:rockon:
i'll always prefer agar but:rockon:
try start your lc from a wedge to a real lc medium, i find it stores better
and by your logic his ms to grain is still faster lol


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Edited by cronicr (01/03/14 01:16 PM)


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Re: Next logical step after PF [Re: cronicr]
    #19363442 - 01/03/14 01:26 PM (10 years, 27 days ago)

and you can still can get piggy backed contams on your agar and transfer to your grains so at the end of the day there's always a risk, lets not pretend that agar will never get you contams, its better than GLC but not by much


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Re: Next logical step after PF [Re: loki44]
    #19363449 - 01/03/14 01:27 PM (10 years, 27 days ago)

your really gonna bring up contams as an arguement here...:rolleyes:


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Re: Next logical step after PF [Re: cronicr]
    #19363465 - 01/03/14 01:32 PM (10 years, 27 days ago)

hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:


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Re: Next logical step after PF [Re: loki44]
    #19363466 - 01/03/14 01:33 PM (10 years, 27 days ago)

There are simple ways to avoid piggyback contams on agar, just most people are either to lazy to do it or just don't know how. Conversely GLC is super risky IMO, just cause a jar looks clean does not mean it is. There is a reason we smell jars before spawning or after G2G. Even then it is very possible for contams like mold which will not be easy to smell through your filter to be hiding in the centre.

Anyone who has run over 100 quarts knows what I'm talking about.


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Re: Next logical step after PF [Re: loki44]
    #19363478 - 01/03/14 01:36 PM (10 years, 27 days ago)

Quote:

loki44 said:
hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:



the needle is only half the issue, who's to say your ms inoculated master is 100% clean:smirk:


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363485 - 01/03/14 01:37 PM (10 years, 27 days ago)

ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?


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Re: Next logical step after PF [Re: loki44]
    #19363488 - 01/03/14 01:38 PM (10 years, 27 days ago)

Quote:

loki44 said:
hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:



Most contams that spread during SAB work do so through contact. A syringe needle makes contact with the inside of the jar via the SHIP, your scalpel does not. But what do I know, I only do 30-40 quarts a month :shrug:


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Re: Next logical step after PF [Re: loki44]
    #19363502 - 01/03/14 01:40 PM (10 years, 27 days ago)

Quote:

loki44 said:
ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?



loki your a one man army here lol, i like lc's but your arguing for nuthing,had you started from a wedge for your original jar and not tried to use faulty logic i would have said.....
:manofapproval:
but like i said faulty logic isn't gonna win ya this one:shocked:


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363514 - 01/03/14 01:43 PM (10 years, 27 days ago)

ok, so where do you think the contams are coming from, because you are not using inoc, just agar wedges and g2g right?


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Re: Next logical step after PF [Re: loki44]
    #19363523 - 01/03/14 01:45 PM (10 years, 27 days ago)

Quote:

loki44 said:
ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?



Not sure what your referring to. My last contam was after the third flush, which I was tempted to blame on my spawn. But after some consideration I decided if my spawn was to blame, that my inoculate was clean and it was my filter that let me down. I have now switched all my filters to SFD and it seems to have corrected the issue. However using an ms syringe is often a risky way to inoculate,  and to make a LC from one be it a GLC or LC from spores, you still run the risk of the contams from the original ms syringe contaming your culture.


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363529 - 01/03/14 01:47 PM (10 years, 27 days ago)

:whathesaid:
i'm not saying your method doesn't work so chill out, just stop saying that it's better then agar because you have no argument there lol


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Re: Next logical step after PF [Re: Pastywhyte]
    #19363530 - 01/03/14 01:47 PM (10 years, 27 days ago)

Also I have used ms syringes to nock up jars, I just dont do it much anymore.


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Re: Next logical step after PF [Re: loki44]
    #19363531 - 01/03/14 01:48 PM (10 years, 27 days ago)

Quote:

loki44 said:
hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now :smirk:



after well over 100 G2G transfers I have yet to see one contamination.
However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.


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Re: Next logical step after PF [Re: cronicr]
    #19363537 - 01/03/14 01:50 PM (10 years, 27 days ago)

Quote:

cronicr said:
Quote:

loki44 said:
ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?



loki your a one man army here lol, i like lc's but your arguing for nuthing,had you started from a wedge for your original jar and not tried to use faulty logic i would have said.....
:manofapproval:
but like i said faulty logic isn't gonna win ya this one:shocked:




ha! faulty logic? whats the probability of an apparently clean colonized grain jar is contamed vs the probability of an apparently clean agar cultured is contamed?

you tell me? if the gap is that wide, because from that point on a needle inoc is less risky than blowing the lids of your sterile jars to drop stuff in


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Re: Next logical step after PF [Re: loki44]
    #19363542 - 01/03/14 01:51 PM (10 years, 27 days ago)

:rolleyes: keep up the good work loki:thumbup:
:leaving:


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Re: Next logical step after PF [Re: MudaFuka]
    #19363547 - 01/03/14 01:53 PM (10 years, 27 days ago)

Quote:

MudaFuka said:

after well over 100 G2G transfers I have yet to see one contamination.
However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.





since you dint mentioned, I'm going to go out in a limb and guess you didn't use GLC, so that was prbly your issue


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Re: Next logical step after PF [Re: cronicr]
    #19363567 - 01/03/14 01:57 PM (10 years, 27 days ago)

Quote:

cronicr said:
:rolleyes: keep up the good work loki:thumbup:
:leaving:





funny thing is I have a bottle of solidified agar and a few sterilized glass petris for a week now, and haven't gotten around to pour them lol

you think agar will still be viable? the  bottle has a SFD and covered with foil LOL


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Re: Next logical step after PF [Re: loki44]
    #19363579 - 01/03/14 02:00 PM (10 years, 27 days ago)

yep probly is:thumbup:
try a lc from a wedge, i use them all the time for my exotics:thumbup:
i've also just squirted water in a colonized"dish"(i used a spice jar)and sucked it back up:cool:


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Re: Next logical step after PF [Re: loki44]
    #19363582 - 01/03/14 02:00 PM (10 years, 27 days ago)

Quote:

loki44 said:
Quote:

MudaFuka said:

after well over 100 G2G transfers I have yet to see one contamination.
However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.





since you dint mentioned, I'm going to go out in a limb and guess you didn't use GLC, so that was prbly your issue



no I didn't. I was just trying to illustrate the fact that it is quite possible to open a jar without contaminating it and that syringes are not always 100% safe. I'm not trying to get into an argument here I'm simply stating what I feel works best.


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Re: Next logical step after PF [Re: cronicr]
    #19363590 - 01/03/14 02:02 PM (10 years, 27 days ago)

Yep my best lc was done that way :super: even picked up some of the tint from the food coloring in the agar and grew pretty thick in the syringe as well.


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Re: Next logical step after PF [Re: cronicr]
    #19363606 - 01/03/14 02:06 PM (10 years, 27 days ago)

Quote:

cronicr said:
yep probly is:thumbup:
try a lc from a wedge, i use them all the time for my exotics:thumbup:
i've also just squirted water in a colonized"dish"(i used a spice jar)and sucked it back up:cool:




when you say LC from a wedge is just the wedge mixed with water or it has some nutrients like honey or LME?


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Re: Next logical step after PF [Re: loki44]
    #19363611 - 01/03/14 02:07 PM (10 years, 27 days ago)

yep use some nutes like honey or karo or dex or me, i use dex/me


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Re: Next logical step after PF [Re: cronicr]
    #19363635 - 01/03/14 02:13 PM (10 years, 27 days ago)

Quote:

cronicr said:
clean cultures,better storage,awesome expanding,cheap,easy,shroomery approved...agar in a nutshell:rockon:



:whathesaid:

agar is way easier then the needless step of making a GLC(grain liquid culture)

I draw up blanks trying to find a reason to use one. Inoculate with a wedge skip the step of making the LC since you will want to make a LC from agar anyway. Then G2G the jar out and avoid syringes alltogether.

LC can be handy sometimes for cubes but you can decide that for yourself after you get to the point at which you would feel comfortable experimenting with it after you have good sterile techniques using agar
agar is super easy, the first few times you practice with it are the hard part.


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Re: Next logical step after PF [Re: bodhisatta]
    #19363820 - 01/03/14 03:09 PM (10 years, 27 days ago)

Quote:

bodhisatta said:
Quote:

cronicr said:
clean cultures,better storage,awesome expanding,cheap,easy,shroomery approved...agar in a nutshell:rockon:



:whathesaid:

agar is way easier then the needless step of making a GLC(grain liquid culture)

I draw up blanks trying to find a reason to use one. Inoculate with a wedge skip the step of making the LC since you will want to make a LC from agar anyway. Then G2G the jar out and avoid syringes all together.

LC can be handy sometimes for cubes but you can decide that for yourself after you get to the point at which you would feel comfortable experimenting with it after you have good sterile techniques using agar
agar is super easy, the first few times you practice with it are the hard part.





You can nock up more jars per petri dish with wedge>mini grain jar>glc = 100's of jars

Its less expansion than wedge>1 jar>8 jars> 64 jars = 100's of jars

Yet with the grain step you can give a chance for contams to show unlike LC which will hide them.


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Edited by mushrume man (01/03/14 03:10 PM)


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Re: Next logical step after PF [Re: mushrume man]
    #19365114 - 01/03/14 06:58 PM (10 years, 27 days ago)

Anyway, I was just thinking of doing a simple experiment. Having only used PF jars in the past, how would just taking a bunch of verm and BRF (maybe with some peat moss), putting it inside an aluminum tray, PC, and then simply inoculating the verm tray, and when it's ready throwing into shotgun. Would this work? It's kind of just a thought, but it seems pretty easy, unless I can find an equally simple way of using things like bird seed. I never built a monotub, and I haven't found as many tutorials or videos on it as the millions that are about PF. It's not that that would stop me, just makes it easier when you have a visual guide to exactly what to do. I remember my first time with PF I kept thinking I'd fuck up, dunking and rolling being such big deals, and then after a while its just pretty much second nature. But that's with a lot of good help and advice, otherwise I wouldn't even know to dunk, for example.

As for this inoculant debate, I'm not as concerned about that at the moment, more so about the fruiting


Edited by Fractal420 (01/03/14 07:13 PM)


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Re: Next logical step after PF [Re: Fractal420]
    #19365169 - 01/03/14 07:08 PM (10 years, 27 days ago)

lol yeah sorry for the thread jack lol
but no the spores won't germinate on verm, just use some cakes to knock up a mono


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Re: Next logical step after PF [Re: cronicr]
    #19365201 - 01/03/14 07:14 PM (10 years, 27 days ago)

Does it matter whether I use regular BRF cakes or should I use WBS jars? Either way, why wouldn't BRF/Verm in a tray work?


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Re: Next logical step after PF [Re: Fractal420]
    #19365217 - 01/03/14 07:17 PM (10 years, 27 days ago)

it will work, cakes are a great place to start spawning...oh okay i see your edit now lol!
you want something that is as tall as it is wide for cakes because the myc grows out in all directions and you will have better results just simply making regular cakes and spawning them to trays of bulk for your sgfc


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Re: Next logical step after PF [Re: cronicr]
    #19365286 - 01/03/14 07:30 PM (10 years, 27 days ago)

Quote:

cronicr said:
it will work, cakes are a great place to start spawning...oh okay i see your edit now lol!
you want something that is as tall as it is wide for cakes because the myc grows out in all directions and you will have better results just simply making regular cakes and spawning them to trays of bulk for your sgfc




Sounds like a plan! Should I use a bunch of small trays or 1 just about the size of the SGFC? (I believe something like 77qt) I was getting good results off of just 10 cakes and I really like the SGFC


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Re: Next logical step after PF [Re: Fractal420]
    #19365301 - 01/03/14 07:32 PM (10 years, 27 days ago)

you can go either way but i would play it safe and do a few trays


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Re: Next logical step after PF [Re: cronicr]
    #19365347 - 01/03/14 07:40 PM (10 years, 27 days ago)

why not try a few mini dubs like these. they are made from 2 15Qt. tubs. This is my first time using them but so far so goodhttp://http://


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