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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Next logical step after PF [Re: loki44]
#19363531 - 01/03/14 01:48 PM (10 years, 27 days ago) |
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Quote:
loki44 said: hell yeah, the exposed area of recently flamed needle is 100's of times smaller than a regular or wide mouth mason jar opened to drop an agar wedge or do g2g over and over and over, come on now 
after well over 100 G2G transfers I have yet to see one contamination. However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.
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loki44
non serviam



Registered: 08/01/13
Posts: 225
Last seen: 10 years, 11 days
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Re: Next logical step after PF [Re: cronicr]
#19363537 - 01/03/14 01:50 PM (10 years, 27 days ago) |
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Quote:
cronicr said:
Quote:
loki44 said: ok pasty if agar is so good , why are you getting stealthy mold in some of your jars, where is it coming from?
loki your a one man army here lol, i like lc's but your arguing for nuthing,had you started from a wedge for your original jar and not tried to use faulty logic i would have said.....
 but like i said faulty logic isn't gonna win ya this one
ha! faulty logic? whats the probability of an apparently clean colonized grain jar is contamed vs the probability of an apparently clean agar cultured is contamed?
you tell me? if the gap is that wide, because from that point on a needle inoc is less risky than blowing the lids of your sterile jars to drop stuff in
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: loki44]
#19363542 - 01/03/14 01:51 PM (10 years, 27 days ago) |
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keep up the good work loki
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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loki44
non serviam



Registered: 08/01/13
Posts: 225
Last seen: 10 years, 11 days
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Re: Next logical step after PF [Re: MudaFuka]
#19363547 - 01/03/14 01:53 PM (10 years, 27 days ago) |
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Quote:
MudaFuka said:
after well over 100 G2G transfers I have yet to see one contamination. However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.
since you dint mentioned, I'm going to go out in a limb and guess you didn't use GLC, so that was prbly your issue
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loki44
non serviam



Registered: 08/01/13
Posts: 225
Last seen: 10 years, 11 days
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Re: Next logical step after PF [Re: cronicr]
#19363567 - 01/03/14 01:57 PM (10 years, 27 days ago) |
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Quote:
cronicr said:
keep up the good work loki

funny thing is I have a bottle of solidified agar and a few sterilized glass petris for a week now, and haven't gotten around to pour them lol
you think agar will still be viable? the bottle has a SFD and covered with foil LOL
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: loki44]
#19363579 - 01/03/14 02:00 PM (10 years, 27 days ago) |
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yep probly is try a lc from a wedge, i use them all the time for my exotics i've also just squirted water in a colonized"dish"(i used a spice jar)and sucked it back up
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Next logical step after PF [Re: loki44]
#19363582 - 01/03/14 02:00 PM (10 years, 27 days ago) |
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Quote:
loki44 said:
Quote:
MudaFuka said:
after well over 100 G2G transfers I have yet to see one contamination. However I have injected both pink mold, black mold and bacteria into jars after only 50 or so injections.
since you dint mentioned, I'm going to go out in a limb and guess you didn't use GLC, so that was prbly your issue
no I didn't. I was just trying to illustrate the fact that it is quite possible to open a jar without contaminating it and that syringes are not always 100% safe. I'm not trying to get into an argument here I'm simply stating what I feel works best.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Next logical step after PF [Re: cronicr]
#19363590 - 01/03/14 02:02 PM (10 years, 27 days ago) |
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Yep my best lc was done that way even picked up some of the tint from the food coloring in the agar and grew pretty thick in the syringe as well.
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loki44
non serviam



Registered: 08/01/13
Posts: 225
Last seen: 10 years, 11 days
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Re: Next logical step after PF [Re: cronicr]
#19363606 - 01/03/14 02:06 PM (10 years, 27 days ago) |
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Quote:
cronicr said: yep probly is try a lc from a wedge, i use them all the time for my exotics i've also just squirted water in a colonized"dish"(i used a spice jar)and sucked it back up
when you say LC from a wedge is just the wedge mixed with water or it has some nutrients like honey or LME?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: loki44]
#19363611 - 01/03/14 02:07 PM (10 years, 27 days ago) |
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yep use some nutes like honey or karo or dex or me, i use dex/me
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Next logical step after PF [Re: cronicr]
#19363635 - 01/03/14 02:13 PM (10 years, 27 days ago) |
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Quote:
cronicr said: clean cultures,better storage,awesome expanding,cheap,easy,shroomery approved...agar in a nutshell

agar is way easier then the needless step of making a GLC(grain liquid culture)
I draw up blanks trying to find a reason to use one. Inoculate with a wedge skip the step of making the LC since you will want to make a LC from agar anyway. Then G2G the jar out and avoid syringes alltogether.
LC can be handy sometimes for cubes but you can decide that for yourself after you get to the point at which you would feel comfortable experimenting with it after you have good sterile techniques using agar agar is super easy, the first few times you practice with it are the hard part.
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mushrume man
Sadis Factory



Registered: 11/18/08
Posts: 375
Loc: PNW
Last seen: 2 years, 17 days
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Re: Next logical step after PF [Re: bodhisatta]
#19363820 - 01/03/14 03:09 PM (10 years, 27 days ago) |
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Quote:
bodhisatta said:
Quote:
cronicr said: clean cultures,better storage,awesome expanding,cheap,easy,shroomery approved...agar in a nutshell

agar is way easier then the needless step of making a GLC(grain liquid culture)
I draw up blanks trying to find a reason to use one. Inoculate with a wedge skip the step of making the LC since you will want to make a LC from agar anyway. Then G2G the jar out and avoid syringes all together.
LC can be handy sometimes for cubes but you can decide that for yourself after you get to the point at which you would feel comfortable experimenting with it after you have good sterile techniques using agar agar is super easy, the first few times you practice with it are the hard part.
You can nock up more jars per petri dish with wedge>mini grain jar>glc = 100's of jars
Its less expansion than wedge>1 jar>8 jars> 64 jars = 100's of jars
Yet with the grain step you can give a chance for contams to show unlike LC which will hide them.
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Edited by mushrume man (01/03/14 03:10 PM)
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Fractal420
Psycellium



Registered: 06/21/13
Posts: 5,913
Last seen: 8 months, 15 days
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Anyway, I was just thinking of doing a simple experiment. Having only used PF jars in the past, how would just taking a bunch of verm and BRF (maybe with some peat moss), putting it inside an aluminum tray, PC, and then simply inoculating the verm tray, and when it's ready throwing into shotgun. Would this work? It's kind of just a thought, but it seems pretty easy, unless I can find an equally simple way of using things like bird seed. I never built a monotub, and I haven't found as many tutorials or videos on it as the millions that are about PF. It's not that that would stop me, just makes it easier when you have a visual guide to exactly what to do. I remember my first time with PF I kept thinking I'd fuck up, dunking and rolling being such big deals, and then after a while its just pretty much second nature. But that's with a lot of good help and advice, otherwise I wouldn't even know to dunk, for example.
As for this inoculant debate, I'm not as concerned about that at the moment, more so about the fruiting
Edited by Fractal420 (01/03/14 07:13 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: Fractal420]
#19365169 - 01/03/14 07:08 PM (10 years, 27 days ago) |
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lol yeah sorry for the thread jack lol but no the spores won't germinate on verm, just use some cakes to knock up a mono
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fractal420
Psycellium



Registered: 06/21/13
Posts: 5,913
Last seen: 8 months, 15 days
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Re: Next logical step after PF [Re: cronicr]
#19365201 - 01/03/14 07:14 PM (10 years, 27 days ago) |
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Does it matter whether I use regular BRF cakes or should I use WBS jars? Either way, why wouldn't BRF/Verm in a tray work?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: Fractal420]
#19365217 - 01/03/14 07:17 PM (10 years, 27 days ago) |
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it will work, cakes are a great place to start spawning...oh okay i see your edit now lol! you want something that is as tall as it is wide for cakes because the myc grows out in all directions and you will have better results just simply making regular cakes and spawning them to trays of bulk for your sgfc
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fractal420
Psycellium



Registered: 06/21/13
Posts: 5,913
Last seen: 8 months, 15 days
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Re: Next logical step after PF [Re: cronicr]
#19365286 - 01/03/14 07:30 PM (10 years, 27 days ago) |
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Quote:
cronicr said: it will work, cakes are a great place to start spawning...oh okay i see your edit now lol! you want something that is as tall as it is wide for cakes because the myc grows out in all directions and you will have better results just simply making regular cakes and spawning them to trays of bulk for your sgfc
Sounds like a plan! Should I use a bunch of small trays or 1 just about the size of the SGFC? (I believe something like 77qt) I was getting good results off of just 10 cakes and I really like the SGFC
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Next logical step after PF [Re: Fractal420]
#19365301 - 01/03/14 07:32 PM (10 years, 27 days ago) |
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you can go either way but i would play it safe and do a few trays
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Next logical step after PF [Re: cronicr]
#19365347 - 01/03/14 07:40 PM (10 years, 27 days ago) |
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why not try a few mini dubs like these. they are made from 2 15Qt. tubs. This is my first time using them but so far so goodhttp:// http://
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