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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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wtf?!
#19354807 - 01/01/14 01:50 PM (10 years, 29 days ago) |
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Wow went to check on my jars today and they all looked great for being over packed and wrong jars. looked a couple hours later one jar was pinsetting and on 20% cultivated so I thought...I freaked and wanted to get it out. wouldn't come out so I broke the jar open..it wasn't even cultivated in the center...the strand is Brazilian but the others aren't doing that. I know this is a ruined jar but what would cause this to happen! It isn't cold by any means so wtf happened?? Help please?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Early pinning usually points to a contam. It's trying to push out fruits while it has the chance.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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What kind of contam I seen none but I went ahead and dunked it for the fun of it. here is it dunked for a few mins
 the blue is obviously a pin but I seen no contam what so ever and smells good
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Can't really see much from the picture but it could be anything there are so many different kinds of molds and bacteria.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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Anyway I could save a tissue sample or something to save the strand? Or is that dangerous because it could be contam?
-------------------- You think your hot shit but your just a cold turd in the sun! (;
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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if you got agar go for it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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You could use agar and try to clean it up by making multiple transfers.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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You mean break it up and use a agar tek?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Transfer a piece to an agar plate and then keep transferring a piece from the leading edge of the new growth to new dishes until you get a clean culture.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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Icyus
KavitārkikasiṃHa



Registered: 11/07/13
Posts: 3,502
Loc: Inbetween.
Last seen: 8 years, 27 days
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Mold is a fungus too?
-------------------- And thus begins the reverse-fusing of our one-dimentional understanding, and adds ever-expanding perspectives, in depth and number; splitting our perception, and in so doing, seemingly irrationally, creates yet more one-ness, with all that ever was, is and will ever be, streching across the infinite, inunderstood concept of everything, percievable and not.
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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Would it not be easier to take my syringe with sterile water inside and stick it through the pin that is already developed to get a tissue sample and make a syringe that way or am I complete off and wrong?
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: wtf?! [Re: Icyus]
#19354893 - 01/01/14 02:15 PM (10 years, 29 days ago) |
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Yes mold is a fungus.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Quote:
visualexplorer said: Would it not be easier to take my syringe with sterile water inside and stick it through the pin that is already developed to get a tissue sample and make a syringe that way or am I complete off and wrong?
Would be much safer to use agar.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
visualexplorer said: Would it not be easier to take my syringe with sterile water inside and stick it through the pin that is already developed to get a tissue sample and make a syringe that way or am I complete off and wrong?
OFF AND WRONG! lol just put it on some agar or a mix of brf and water(or if you have spores just knock more jars up)
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Icyus
KavitārkikasiṃHa



Registered: 11/07/13
Posts: 3,502
Loc: Inbetween.
Last seen: 8 years, 27 days
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What was your purpose? Grow fungi? Then be happy! You have done so... you have succeded!
-------------------- And thus begins the reverse-fusing of our one-dimentional understanding, and adds ever-expanding perspectives, in depth and number; splitting our perception, and in so doing, seemingly irrationally, creates yet more one-ness, with all that ever was, is and will ever be, streching across the infinite, inunderstood concept of everything, percievable and not.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: wtf?! [Re: Icyus]
#19354909 - 01/01/14 02:19 PM (10 years, 29 days ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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Okay then then take my p.o.s. cake crumble it up and put it in a agar mixture or another brf mixture to start recultivation ?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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take 5 grams of brf and mix with 16 cc's of water, mix it up and pour it in the bottom of a half pint with a filtered lid, i use poly for my lid, pc for 20-40 minutes and let it cool, take a piece of fruit from the center close the base and drop it in there, wait for colonization transfer to a new dish
 i used baby food jars
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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visualexplorer
whiteymyc


Registered: 12/19/13
Posts: 160
Loc: upup&away
Last seen: 7 years, 6 months
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I have no fruit though just one pin. I thought I would be doing something closer to a jar to box transfer kind of thing seeing as there is no visual contam would that not be easier or is it to risky?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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so drop the pin on there lol, and i thought this was all because of a contam
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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