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OfflineYuri.Pono
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Yuri's Agar Work
    #19354229 - 01/01/14 10:33 AM (10 years, 29 days ago)

So this is gonna be where i'll post my agar attempts both positives attempts, and failures. so i can learn from my mistakes, keep all the good info you guys direct me to until im a Agar PRO!

so to start with I'm gonna go with a PE6 print i received not to long ago. my plan is to swipe a spot on the print then transfer to agar. my agar will be a recipe of MYA = Malt-yeast Extract

  • H2O 500 mL

  • Agar 10g

  • Malt 10g

  • Yeast 1g


dry ingredients are being mixed now. then added to boiling water for 1 min. then mixed, and poured to agar round dishes


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Edited by Yuri.Pono (01/01/14 11:35 AM)


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19354309 - 01/01/14 11:09 AM (10 years, 29 days ago)

:thumbup:
i'm proud of you:rockon:


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19354491 - 01/01/14 12:07 PM (10 years, 29 days ago)

so i just done my mix and letting the agar set up in the rounds to cool. got to drill out my holes while waiting then ill PC for 45 min @ 15 psi.

now from what im reading im letting them setup after my pour will help with condensation? and how long after they cool do i wait to PC?


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Edited by Yuri.Pono (01/01/14 12:08 PM)


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19354503 - 01/01/14 12:11 PM (10 years, 29 days ago)

after i pour mine i wait till it gets back to room temp before putting the lid on and pc'ing

thts right after i poured and you can see hte little condensation there, i wait for tht to go away and close the lid for a few minutes to see if any more builds up and repeat till i know it's at room temp


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19354602 - 01/01/14 12:48 PM (10 years, 29 days ago)

:thumbup: thanks will do


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Re: Yuri's Agar Work [Re: cronicr]
    #19354637 - 01/01/14 01:00 PM (10 years, 29 days ago)

Quote:

cronicr said:
after i pour mine i wait till it gets back to room temp before putting the lid on and pc'ing

thts right after i poured and you can see hte little condensation there, i wait for tht to go away and close the lid for a few minutes to see if any more builds up and repeat till i know it's at room temp



I wish I knew about this a while ago my pp5s are fogy ass hell.
:themoreyouknow:


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Re: Yuri's Agar Work [Re: MudaFuka]
    #19354639 - 01/01/14 01:01 PM (10 years, 29 days ago)

:thumbup:
also helps to leave them in the pc to cool:cool:


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Re: Yuri's Agar Work [Re: cronicr]
    #19354697 - 01/01/14 01:16 PM (10 years, 29 days ago)

thanx for the tip. hopefully if I read enough of these threads ill actually have some success with agar. Iv had so many bacterial contams im starting to lose my fucking minde.


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Re: Yuri's Agar Work [Re: MudaFuka]
    #19354710 - 01/01/14 01:19 PM (10 years, 29 days ago)

i had the same problems for awhile, just remember the more you can keep the temps the same on the outside as in the less condensation you will have




so even if you take it out of the pc to quick you will end up with it:thumbup:


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19354799 - 01/01/14 01:48 PM (10 years, 29 days ago)

so far what im working with.



got a dinner date with the wife's family so im gonna just let them sit until i get back then PC.

for transfering my spores im gonna take a Q-Tip and cut 1 end off, and then take a bamboo stick and tape the 2 together. wrap in foil and PC with the agar. let it all cool over night in the PC then get to work in the morning.

do i need sterile water? or can i just dab the swab on the print and streak the agar?


just got to thinking of what i can do after i cant use these anymore. they'd make great psilly jello shot for next halloween.


Edited by Yuri.Pono (01/01/14 01:54 PM)


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19354820 - 01/01/14 01:53 PM (10 years, 29 days ago)

you can just dab it on the print


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19354824 - 01/01/14 01:54 PM (10 years, 29 days ago)

oh and:rockon:
Quote:

Yuri.Pono said:
so far what im working with.








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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19354836 - 01/01/14 01:57 PM (10 years, 29 days ago)

cool so i'll streak 2 rounds  clean them both up. then transfer to grain jars. g2g then spawn to bulk. find me some nice clone to put to agar and repeat to fine the strongest isolate.


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19362483 - 01/03/14 08:56 AM (10 years, 28 days ago)

cooked agar last night. was hoping to not have condensation on the walls as i did let it cool to room temp over night both after pour and after cooking.

on another note i'm gonna work on my PE6, and clone Escondido.







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Edited by Yuri.Pono (01/03/14 09:44 AM)


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19362611 - 01/03/14 09:47 AM (10 years, 28 days ago)

hope if i stick these on their side that the condensation will pool up like on my glass one and ill pour out b4 working. as they weren't terribly bad as the glass ones was.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19363104 - 01/03/14 12:03 PM (10 years, 27 days ago)

conedensation is not the end of the world itf you leave it for a couple days it will get absorbed back into the agar, sometimes it's just impossible to avoid and i think it has something to do with how fast we let the pressure drop when we shut the pc off, but like i said it's no biggies anyway:thumbup:


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19374745 - 01/05/14 08:48 PM (10 years, 25 days ago)

question. i havent got to my agar work but plan to in the am Thursday if not b4. work is kicking myass atm. but i do pick 3 fruit for cloning all 3 come from cluster and are meaty. i double bagged them and tossed in frig until then will that be ok?



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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19374753 - 01/05/14 08:54 PM (10 years, 25 days ago)

If they are in plastic they will go bad fast. I believe a paper bag would be best.


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: magickspore]
    #19374812 - 01/05/14 09:11 PM (10 years, 25 days ago)

shit that sucks no brown bags here. how about tyvek bag?


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19374814 - 01/05/14 09:11 PM (10 years, 25 days ago)

worst comes to worst the agar will go to my PE6 print


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19374831 - 01/05/14 09:16 PM (10 years, 25 days ago)

anything breathable


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: cronicr]
    #19374849 - 01/05/14 09:19 PM (10 years, 25 days ago)

ill toss some polt fil in it and leave til bag open a tad


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19374992 - 01/05/14 09:53 PM (10 years, 25 days ago)

Paper towels work too, just throw it in the crisper in the fridge :P


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Re: Yuri's Agar Work [Re: KnownPown]
    #19383945 - 01/07/14 05:35 PM (10 years, 23 days ago)

agar work started in the morning. cleaning up kitchen tonight. can't wait til the fun begins.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19384040 - 01/07/14 05:51 PM (10 years, 23 days ago)

I have got to comment here:

I Love using sterilized qtips for the limited agar work I have done.  I generally cram a few dozen into a small mouth half pint to sterilize.  They can go in with the agar as well....I can not see any reason why 20-40 min with agar at 15psi should not sterilize them.  That is my spore to agar inoculation "loop".  Thumbs up!


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: HypnotoadCroaked]
    #19384088 - 01/07/14 05:59 PM (10 years, 23 days ago)

thanks keep an eye out to help guide me if you will


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19384112 - 01/07/14 06:03 PM (10 years, 23 days ago)

Quote:

Yuri.Pono said:
thanks keep an eye out to help guide me if you will



All I do is take a little wipe from my print in the SAB, and just make a single dab in the middle of the agar.  One little wipe of the print can actually satisfy more than a few jars.  I will certainly keep an eye out here.  I don't know much, but I know what I have done has worked splendid so far.


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Re: Yuri's Agar Work [Re: HypnotoadCroaked]
    #19384118 - 01/07/14 06:04 PM (10 years, 23 days ago)

:whistling:


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Re: Yuri's Agar Work [Re: cronicr]
    #19384187 - 01/07/14 06:20 PM (10 years, 23 days ago)

i know. i know. i said id get at them days ago but im on it for real. Loving the LIGHT. been down a dark road lately don't like where it is taking me. Flowhood Project in the works:mushroom2:


Edited by Yuri.Pono (01/07/14 06:23 PM)


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19403137 - 01/11/14 10:35 AM (10 years, 19 days ago)

Do you guys ever PC your tools b4 working? or do you just flame sterilize the scalpel? also for folks that reuse your sleeves do you PC them b4 reuse?


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19403164 - 01/11/14 10:42 AM (10 years, 19 days ago)




2 rounds i opened for 2 min just to see what would grow


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19403183 - 01/11/14 10:45 AM (10 years, 19 days ago)

today im transferring a agar wedge that made it's way to my collection to agar today. then ill move it to grain and another plater. also trying to do my wikidzon print to agar since i have more of them then the pe6. cant wait for my Hepa filter to get here.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19403257 - 01/11/14 11:02 AM (10 years, 19 days ago)

I never sterilize a scalpel or inoc loop because they are going to be handled with iso soaked gloves and the tip flamed red hot anyway.

I just find it redundant to do so.


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: bootster]
    #19403880 - 01/11/14 01:29 PM (10 years, 19 days ago)

not much to see got a wedge and a clone following this method




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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19404021 - 01/11/14 02:03 PM (10 years, 19 days ago)

:popcorn:


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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Re: Yuri's Agar Work [Re: FrankHorrigan]
    #19404029 - 01/11/14 02:05 PM (10 years, 19 days ago)

Quote:

FrankHorrigan said:
:popcorn:




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Re: Yuri's Agar Work [Re: cronicr]
    #19409863 - 01/12/14 07:49 PM (10 years, 18 days ago)

9" oval cup curette

cool little toy im gonna try out for agar. seen at another site. dude says it scoops agar cultures nicely.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19409868 - 01/12/14 07:50 PM (10 years, 18 days ago)

40 bucks for a little spoon?


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19409871 - 01/12/14 07:50 PM (10 years, 18 days ago)

also tomorrow im gonna try and get me some picture of that agar shots every thing is recovering nicely.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19409873 - 01/12/14 07:51 PM (10 years, 18 days ago)

found one for $10


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19411755 - 01/13/14 09:45 AM (10 years, 18 days ago)

posting pictures here in a few trying to figure out how w/o opening them up. wedge is doing good but the other i think has got bacteria in it.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19411771 - 01/13/14 09:52 AM (10 years, 18 days ago)

:waitingpatiently:


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Re: Yuri's Agar Work [Re: cronicr]
    #19411981 - 01/13/14 11:12 AM (10 years, 17 days ago)

Quote:

cronicr said:
:waitingpatiently:




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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: FrankHorrigan]
    #19412974 - 01/13/14 03:41 PM (10 years, 17 days ago)


best shots i can get


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19413522 - 01/13/14 05:52 PM (10 years, 17 days ago)

looking good yuri!


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Offlinetwistedty
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Re: Yuri's Agar Work [Re: cronicr]
    #19413739 - 01/13/14 06:28 PM (10 years, 17 days ago)

:thumbup:


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: twistedty]
    #19421691 - 01/15/14 07:47 AM (10 years, 16 days ago)

GT WEDGE





CLONE ES


NOTHING GOOD WITH THE CLONE


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19427425 - 01/16/14 10:58 AM (10 years, 14 days ago)



and check out this beauty


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InvisibleFrankHorrigan
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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19427543 - 01/16/14 11:30 AM (10 years, 14 days ago)

Looking good! And nice hood man!

:congrats:


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Re: Yuri's Agar Work [Re: FrankHorrigan]
    #19427810 - 01/16/14 12:28 PM (10 years, 14 days ago)

:whathesaid:


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19436849 - 01/18/14 08:53 AM (10 years, 13 days ago)


GT ISOLATE

going to be transferring to new agar this week under the new flowhood.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19540963 - 02/08/14 06:05 PM (9 years, 11 months ago)

so back to my agar work. now that my flowhood is built time to get to work. i have a few prints id like to work but now i have a few questions. when doing agar in front of a flowhood how do you do your prep work? i seen where someone sprays the filter with Lysol which to me wouldnt be a good idea or would it? i know wipe everything thing with 70% iso, wear tyvek sleeves fresh laundered clothes. all my rooms are big. and i dont want to work in the bathroom is that a problem?


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Edited by Yuri.Pono (02/08/14 06:07 PM)


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19586689 - 02/18/14 06:55 PM (9 years, 11 months ago)



GT1, GT2 both had 2 spots pop up 2 days ago and i transfered 5 days ago so im assuming contams and transfered tonight to new dish pictures on them in  a few days.

ES from the start of transfer had bacteria in 2 spot transfered tonight also. pictures in a few days.

ran 1 agar dish during the whole time of all 3 original dish work no cantams so something wrong on my end while working in front of the hood.


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Edited by Yuri.Pono (02/18/14 07:01 PM)


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19586715 - 02/18/14 07:02 PM (9 years, 11 months ago)

also during the transfer from these plates ran 2 more agar dishes with nothing in them just for testing again.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19586775 - 02/18/14 07:15 PM (9 years, 11 months ago)

hoping my 8 jars that i transfered GT agar to are good as they are taking off fine ill give a little shake here soon will post another journal for that later on this week.


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Re: Yuri's Agar Work [Re: Yuri.Pono]
    #19591822 - 02/19/14 08:11 PM (9 years, 11 months ago)

Looking good so far, keep it up :thumbup:


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InvisibleFrankHorrigan
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Re: Yuri's Agar Work [Re: Pastywhyte]
    #19594504 - 02/20/14 12:31 PM (9 years, 11 months ago)

Looks good so far, I agree with Pasty. If you are having issues that you think are related to your hood, work in your SAB and see if your contam rate improves. 

A little bacteria on your plate is to be expected every now and then though :wink:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


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OfflineYuri.Pono
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Re: Yuri's Agar Work [Re: FrankHorrigan]
    #19601274 - 02/21/14 07:12 PM (9 years, 11 months ago)

hey Frank how are you beating  the bacteria? ive done 2 plate with a transfer and both showed bacteria the very next day. so i transfered again and same thing


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