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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19411755 - 01/13/14 09:45 AM (10 years, 17 days ago) |
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posting pictures here in a few trying to figure out how w/o opening them up. wedge is doing good but the other i think has got bacteria in it.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19411771 - 01/13/14 09:52 AM (10 years, 17 days ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Yuri's Agar Work [Re: cronicr]
#19411981 - 01/13/14 11:12 AM (10 years, 17 days ago) |
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Quote:
cronicr said:

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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19413522 - 01/13/14 05:52 PM (10 years, 17 days ago) |
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looking good yuri!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: Yuri's Agar Work [Re: cronicr]
#19413739 - 01/13/14 06:28 PM (10 years, 17 days ago) |
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: twistedty]
#19421691 - 01/15/14 07:47 AM (10 years, 16 days ago) |
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GT WEDGE




CLONE ES

 NOTHING GOOD WITH THE CLONE
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19427425 - 01/16/14 10:58 AM (10 years, 14 days ago) |
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and check out this beauty
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19427543 - 01/16/14 11:30 AM (10 years, 14 days ago) |
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Looking good! And nice hood man!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19436849 - 01/18/14 08:53 AM (10 years, 13 days ago) |
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 GT ISOLATE
going to be transferring to new agar this week under the new flowhood.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19540963 - 02/08/14 06:05 PM (9 years, 11 months ago) |
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so back to my agar work. now that my flowhood is built time to get to work. i have a few prints id like to work but now i have a few questions. when doing agar in front of a flowhood how do you do your prep work? i seen where someone sprays the filter with Lysol which to me wouldnt be a good idea or would it? i know wipe everything thing with 70% iso, wear tyvek sleeves fresh laundered clothes. all my rooms are big. and i dont want to work in the bathroom is that a problem?
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (02/08/14 06:07 PM)
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19586689 - 02/18/14 06:55 PM (9 years, 11 months ago) |
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GT1, GT2 both had 2 spots pop up 2 days ago and i transfered 5 days ago so im assuming contams and transfered tonight to new dish pictures on them in a few days.
ES from the start of transfer had bacteria in 2 spot transfered tonight also. pictures in a few days.
ran 1 agar dish during the whole time of all 3 original dish work no cantams so something wrong on my end while working in front of the hood.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
Edited by Yuri.Pono (02/18/14 07:01 PM)
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19586715 - 02/18/14 07:02 PM (9 years, 11 months ago) |
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also during the transfer from these plates ran 2 more agar dishes with nothing in them just for testing again.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19586775 - 02/18/14 07:15 PM (9 years, 11 months ago) |
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hoping my 8 jars that i transfered GT agar to are good as they are taking off fine ill give a little shake here soon will post another journal for that later on this week.
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Yuri's Agar Work [Re: Yuri.Pono]
#19591822 - 02/19/14 08:11 PM (9 years, 11 months ago) |
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Looking good so far, keep it up
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Looks good so far, I agree with Pasty. If you are having issues that you think are related to your hood, work in your SAB and see if your contam rate improves.
A little bacteria on your plate is to be expected every now and then though
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Yuri.Pono
MAD SCIENTIST



Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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hey Frank how are you beating the bacteria? ive done 2 plate with a transfer and both showed bacteria the very next day. so i transfered again and same thing
-------------------- Links To Success finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8 want to chat? got xxmp/pidgin/otr PM me for info
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