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Offlineshirley knott
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green
    #1935155 - 09/20/03 12:01 PM (13 years, 2 months ago)

looks okay at first

til you look in the window and find this:

and a second, more obvious jar -


jars were shaken immediately after inoculation by spore syringe through the smaller of two holes. injection port sellotaped after innoc.
so obviously it's coming in the top, but where? can it come through the tyvek? (2 layers, thin kite-making tyvek). off the sellotape? anything i should check?



i've lost quite a few recently to this, or a lookalike. any suggestions? i have no flowhood yet.


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InvisibleEffedS
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Re: green [Re: shirley knott]
    #1935169 - 09/20/03 12:10 PM (13 years, 2 months ago)

Hippie's Bleach Tek?


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OfflineMAIA
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Re: green [Re: Effed]
    #1935247 - 09/20/03 01:02 PM (13 years, 2 months ago)

Hippie's bleach tek is a good one Effed but it's to be used with fully colonized substrate.
There's not much you can do now, toss the jar, it's lost.

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlineshirley knott
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Re: green [Re: MAIA]
    #1935359 - 09/20/03 01:51 PM (13 years, 2 months ago)

yeah, i know that. it's toast. but why am i continually getting it? any clues?


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InvisibleTomandjerry58
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Re: green [Re: shirley knott]
    #1935427 - 09/20/03 02:38 PM (13 years, 2 months ago)

when your knocking them up. what kind of sterilization do you use?


and i have found when u shake a jar its more likely to contam.

im not very experienced with grain but every jar i have shaken has contamed.


Edited by Tomandjerry58 (09/20/03 02:43 PM)


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Offlineshirley knott
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Re: green [Re: Tomandjerry58]
    #1935500 - 09/20/03 03:12 PM (13 years, 2 months ago)

i wish i had a flowhood: i make my own syringes. inoculate jar. tape hole. shake jar. leave alone.


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InvisibleEffedS
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Re: green [Re: shirley knott]
    #1936697 - 09/21/03 01:20 AM (13 years, 2 months ago)

Hehheh sorry I have never even read Hippie's Bleach Tek, I just knew it was used to battle trich in some way.  :smirk:

I noticed also that you dont use polyfill. Why?

I am betting that is part of the problem.

www.fungifun.org 

Ohh I see the Tyvek now.. I still prefer polyfill. It is self sealing. Tyvek leaves a small hole where the syringe was.

Also chances of green are at least 10 fold this time of year, compared to winter.
Just my thoughts.


Edited by Effed (09/21/03 01:24 AM)


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OfflineUncleMike
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Re: green [Re: Effed]
    #1937340 - 09/21/03 08:34 AM (13 years, 2 months ago)

Could be getting in through the big hole onto they tyvek then after shaking the trich. spores found their way in through the innoc. hole in the tyvek....under the lid....try putting a peice of polyfill in the air exchange hole...


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InvisibleSemilanceata
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Re: green [Re: UncleMike]
    #1937576 - 09/21/03 12:01 PM (13 years, 2 months ago)

I can?t see the green. I?m kind of blind though :confused:


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OfflineMAIA
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Re: green [Re: Semilanceata]
    #1938811 - 09/21/03 10:13 PM (13 years, 2 months ago)

Well, the contamination is coming from the top, so i agree with Effed, the culprit could be the tyvek filter.

MAIA


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Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineSeussA
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Re: green [Re: MAIA]
    #1940086 - 09/22/03 10:02 AM (13 years, 2 months ago)

It could also be from spores in the air space or in the threads on the lid that survived sterilization and became airborn after being shaken. Spores could also have been sucked in to fill the low pressure from cooling gasses after sterilization.

Make a jar the same way you have been, but do not inoculate. If it contaminates, then the problem is with your filter or sterilization technique.

Make a jar the same way you have been, but cover the filter with something airtight. Do not inoculate. If it contaminates, then the prbolem is with your sterilization technique.


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Anonymous

Re: green [Re: shirley knott]
    #1940275 - 09/22/03 12:17 PM (13 years, 2 months ago)

hi shirl
i havent done grains yet but ive shaken my brf jars and i read that it causes suction so i always spray the room im doing the shaking in.


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OfflineSuntzu
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Re: green [Re: shirley knott]
    #1943739 - 09/23/03 10:47 AM (13 years, 2 months ago)

Hey Shirly;  I thought I'd throw a couple cents in here, as usual Suess and I are on similar tracks. . .

IME mold spores do not survive PC'ing in quart jars [assuming PC'ing done properly];  Bacillus endospores [wet spot] are far more likely to.  Mold [again, just my opinion] is a result of either poor air quality during inoculation or more often, poor air quality during the COOLING process.  I believe this is an often under-appreciated stage of substrate preparation. 

As jars go from hot to cool, all of the inner air space of both the PC and the jars themselves is replaced by *outside* air.  The filters are there to theoretically filter the new air that enters the jars when cooling.  The vast majority of non-inoculation-related mold contamination can be fixed by cooling the PC in as clean of an area as possible. 

If the problem involves most/all jars, and is not just a sporadic thing, a couple of negative controls can serve you well;

1.  Jar prepared, shaken, but not inoculated.
2.  Jar prepared, shaken, and 'fake inoculated'.

These jars can be inoculated at a later time if no contams arise, so no real waste.

One way or the other, mold contamination is likely an air quality issue.  Again, IME :wink:    Best of luck! 


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour

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