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OfflineYuri.Pono
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19348561 - 12/30/13 08:57 PM (10 years, 1 month ago)

think i'd like to jump in on this. i've never done spore to agar and just happened to get me a PE6 print.

few question. how do i start?

Quote:

make agar
take knock loop run through print to agar





of course i'd  do every thing clean like flame sterilize, and use a glove box. just kinda need the gist of it all. i been reading some.  figured this would be a good place to start.


--------------------
Links To Success
finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8
want to chat? got xxmp/pidgin/otr PM me for info


Edited by Yuri.Pono (12/30/13 08:58 PM)


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
    #19348603 - 12/30/13 09:05 PM (10 years, 1 month ago)

sab not a glove box! you can start with blues agar write up or pasty's or any of the proven teks on here and go from there, you just want some nice clean growth and theres no need to isolate anything just yet for your first time i suggest keeping it simple and clone when your ready:cool:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
    #19348605 - 12/30/13 09:05 PM (10 years, 1 month ago)

Quote:

Yuri.Pono said:
think i'd like to jump in on this. i've never done spore to agar and just happened to get me a PE6 print.

few question. how do i start?

Quote:

make agar
take knock loop run through print to agar





of course i'd  do every thing clean like flame sterilize, and use a glove box. just kinda need the gist of it all. i been reading some.  figured this would be a good place to start.



I use sterilized q-tips for moving spores to agar.  Disposable and cheap.  I put a few dozen in a half pint regular mouth jar, and pc with grain or agar.  I rub my print a bit, than lightly touch the agar.  All it takes is a few spores per plate.....the less the better.  You can do a few dishes with one qtip.

There are many good agar teks, and for someone starting out, use the search function for "no pour agar".  Agar can be pre-loaded into small glass ball jars OR pp5 plastic containers.  Its a great place to start for someone new to agar, or even someone who simply does not feel like pouring into dishes. 

Welcome to the party.  Hope we can help see you through the whole process.

EDIT:  What better way to spend my vacation than to help on the shroomery full time lol.  Staycation for the win!  If I didn't enjoy this forum, Id not be here. 


Edited by HypnotoadCroaked (12/30/13 09:07 PM)


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19348615 - 12/30/13 09:06 PM (10 years, 1 month ago)

i like q-tips, but i find i need longer ones for the halfpints, mine are shorter then i would like


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19348630 - 12/30/13 09:09 PM (10 years, 1 month ago)

Quote:

cronicr said:
i like q-tips, but i find i need longer ones for the halfpints, mine are shorter then i would like



Sterilized Bamboo shish-ka-bob skewers with a frayed up end?


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19348635 - 12/30/13 09:10 PM (10 years, 1 month ago)

:headbang3: lol or just tape it to a pencil lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineYuri.Pono
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19348656 - 12/30/13 09:15 PM (10 years, 1 month ago)

im using pasty's tek with the glad, and micropore tape.  got 24 rounds ready for agar. figured id do the print to agar, then clean agar to wbs, g2g, spawn, pick then clones to agar

thanks for the q-tip idea any of them work brand wise?


--------------------
Links To Success
finger print: A9E348E5 2A22AFB6 F3676613 A34B1454 7C36ABC8
want to chat? got xxmp/pidgin/otr PM me for info


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
    #19348721 - 12/30/13 09:31 PM (10 years, 1 month ago)

Quote:

Yuri.Pono said:
im using pasty's tek with the glad, and micropore tape.  got 24 rounds ready for agar. figured id do the print to agar, then clean agar to wbs, g2g, spawn, pick then clones to agar

thanks for the q-tip idea any of them work brand wise?



as long as you can pc it your good!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Yuri.Pono]
    #19348758 - 12/30/13 09:40 PM (10 years, 1 month ago)

Quote:

Yuri.Pono said:
im using pasty's tek with the glad, and micropore tape.  got 24 rounds ready for agar. figured id do the print to agar, then clean agar to wbs, g2g, spawn, pick then clones to agar

thanks for the q-tip idea any of them work brand wise?



Whatever you got in your medicine cabinet will work great.

And you are correct in your process.  It looks simple and quick when you put it into a single sentence.  Generally 2-3 transfers from agar are suggested for everything from clones to multispore.  After about 2-3 xfers you will be able to then move a small cutting of agar to grain.  So long as its done in the still air box/glove box, you really reduce your chance of issues to nearly 0% if you take your time.  Still air boxes are the best thing ever!


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OfflineKnownPown
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19350016 - 12/31/13 07:03 AM (10 years, 1 month ago)

I just wanted to ask a small question aswell, I have a B+ print I'll be putting to agar by innoc loop and sterilized cotton swabs..

Guess I'll see which I like better.

But my question is that I have EQ and GT syringes which are quite old, 15 months?, and anyways I'd like to put those to agar too.

Was wondering if I could put some spore solution ina shot glass or something and then take a qtip and soak some of that solution, then inoculating a petri with agar? Sounds to me like it'd work, I just wanted to do it so I didnt have to deal with too much liquid solution on my agar...think it'd work? :smile:


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InvisibleHypnotoadCroaked
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Registered: 01/05/13
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: KnownPown]
    #19350487 - 12/31/13 10:03 AM (10 years, 1 month ago)

Quote:

KnownPown said:
I just wanted to ask a small question aswell, I have a B+ print I'll be putting to agar by innoc loop and sterilized cotton swabs..

Guess I'll see which I like better.

But my question is that I have EQ and GT syringes which are quite old, 15 months?, and anyways I'd like to put those to agar too.

Was wondering if I could put some spore solution ina shot glass or something and then take a qtip and soak some of that solution, then inoculating a petri with agar? Sounds to me like it'd work, I just wanted to do it so I didnt have to deal with too much liquid solution on my agar...think it'd work? :smile:



That is entirely possible, but I would certainly do one or 2 with the smallest drop straight from the needle.  After 15 months, anything is possible with syringes.  Most syringes are not 100% clean, and waiting months/years can accelerate the growth of what was minimal when it was created.

Shake them syringes good, and give it a try.


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19350658 - 12/31/13 10:54 AM (10 years, 1 month ago)

Quote:

Whippy said:
Quote:

KnownPown said:
I just wanted to ask a small question aswell, I have a B+ print I'll be putting to agar by innoc loop and sterilized cotton swabs..

Guess I'll see which I like better.

But my question is that I have EQ and GT syringes which are quite old, 15 months?, and anyways I'd like to put those to agar too.

Was wondering if I could put some spore solution ina shot glass or something and then take a qtip and soak some of that solution, then inoculating a petri with agar? Sounds to me like it'd work, I just wanted to do it so I didnt have to deal with too much liquid solution on my agar...think it'd work? :smile:



That is entirely possible, but I would certainly do one or 2 with the smallest drop straight from the needle.  After 15 months, anything is possible with syringes.  Most syringes are not 100% clean, and waiting months/years can accelerate the growth of what was minimal when it was created.

Shake them syringes good, and give it a try.



:whathesaid:
definitely would do a drop or two onto the agar with a needle, and be sure to shake the shit outta those old needles.  good luck! 
also pasty and i did a quick little trial with tetracycline agar (you can find tetracycline at local pet store) i had put some bacteria onto the tetra agar and the bacteria never spread i waited two weeks (i think)...  it won't stop other fungus's or molds but it is a good way to print wild prints, or possibly questionable syringes or prints.


--------------------


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19350664 - 12/31/13 10:56 AM (10 years, 1 month ago)

:headbang3:
just got some aus spores in the mail this morning:rockon:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19350724 - 12/31/13 11:11 AM (10 years, 1 month ago)

Quote:

cronicr said:
:headbang3:
just got some aus spores in the mail this morning:rockon:



Tazmanian?


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: HypnotoadCroaked]
    #19350726 - 12/31/13 11:12 AM (10 years, 1 month ago)

:shrug:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisiblePastywhyteMDiscord
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19350762 - 12/31/13 11:24 AM (10 years, 1 month ago)

Quote:

blueconfusion said:
also pasty and i did a quick little trial with tetracycline agar (you can find tetracycline at local pet store) i had put some bacteria onto the tetra agar and the bacteria never spread i waited two weeks (i think)...  it won't stop other fungus's or molds but it is a good way to print wild prints, or possibly questionable syringes or prints.




Yeah I used it for a few wilds already :rockon: One was super dirty and so far so good :super

Quote:

cronicr said:
:headbang3:
just got some aus spores in the mail this morning:rockon:




From the Olive giveaway?


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OfflinecronicrFacebook
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: Pastywhyte]
    #19350769 - 12/31/13 11:26 AM (10 years, 1 month ago)

nope from strom:thumbup: so probly lol


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: cronicr]
    #19351150 - 12/31/13 01:20 PM (10 years, 1 month ago)

so curiosity and boredom got the better of me today, and i decided to go look at both health food shops to compare prices on agar and rye.  the one closest to my house rye was almost $2.00/ lbs. and a bottle of agar was almost $10.00!!!  the other one that is kinda on my way home from work was a little over a dollar for a pound of rye and agar was $2.00 cheaper!  at least i know where i'm going in a couple weeks for supplies after everything blows over! :super: i can't wait to get back to it!


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Offlineblueconfusion
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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19351160 - 12/31/13 01:23 PM (10 years, 1 month ago)

i have some texas, crs and aussie's gonna be going to agar along with some p. galindoi!  thanx to strom and blackdove!


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Re: Wanna Step Up Your Game? Come Grow Along With Blue! Spore Print to Spore Print [Re: blueconfusion]
    #19351168 - 12/31/13 01:25 PM (10 years, 1 month ago)

john allen strain before the g2g and thats columbian rust spore on the right


psilocybe hispanica


rust spore grains on agar



ksss germinated
loving this thread blue!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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