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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,525
Loc: Maine
Last seen: 10 months, 3 days
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sterile container
#19350247 - 12/31/13 08:38 AM (10 years, 1 month ago) |
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So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.
I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.
If I use a half pint jar, the tall one, and put a sfd with a 1/4" hole for ge, and a ship out of gasket maker,
How long do I PC it to make it sterile? 90 seems too long... Just figured id ask.
Thanks guys :-)
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NOTFALL3N
Epic

Registered: 11/18/13
Posts: 371
Last seen: 3 years, 10 months
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Cover the jar and 90 mins is fine.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Quote:
blojo02184 said: So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.
I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.
Just use the spores as they are to knock up a jar.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: sterile container [Re: PussyFart]
#19350321 - 12/31/13 08:59 AM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
blojo02184 said: So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.
I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.
Just use the spores as they are to knock up a jar.
 Unless you're trying to stretch them out into more syringes.
I PC my agar for 30M so to sterilize water 30M at pressure is sufficient.
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,525
Loc: Maine
Last seen: 10 months, 3 days
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I'm trying to expand the 5cc deep freeze syringe into 20cc syringe so as to stretch it. 30 minutes huh?
Container going in empty, then once sterile injecting the deep freeze, then sterile water. Shake, suck it up and knock some rye up.
Thanks guys
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Quote:
blojo02184 said: I'm trying to expand the 5cc deep freeze syringe into 20cc syringe so as to stretch it. 30 minutes huh?
Container going in empty, then once sterile injecting the deep freeze, then sterile water. Shake, suck it up and knock some rye up.
Thanks guys
Sounds like a good time to experiment with agar.
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