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Offlineblojo02184
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Registered: 05/15/13
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sterile container
    #19350247 - 12/31/13 08:38 AM (10 years, 1 month ago)

So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.

I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.

If I use a half pint jar, the tall one, and put a sfd with a 1/4" hole for ge, and a ship out of gasket maker,

How long do I PC it to make it sterile? 90 seems too long... Just figured id ask.

Thanks guys  :-)


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OfflineNOTFALL3N
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Registered: 11/18/13
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Re: sterile container [Re: blojo02184]
    #19350304 - 12/31/13 08:54 AM (10 years, 1 month ago)

Cover the jar and 90 mins is fine.


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OfflinePussyFart
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Re: sterile container [Re: blojo02184]
    #19350317 - 12/31/13 08:58 AM (10 years, 1 month ago)

Quote:

blojo02184 said:
So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.

I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.



Just use the spores as they are to knock up a jar.


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InvisiblebodhisattaMDiscordReddit
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Re: sterile container [Re: PussyFart]
    #19350321 - 12/31/13 08:59 AM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

blojo02184 said:
So, I got a "deep freeze" syringe from a non-sponsor. Supposedly this syringe is freezable. I believe that that spores are held in a glycol solution so as not to freeze.

I'm making a sterile container to shoot out glycol spores, mix with sterile water, suck up and knock up a jar.



Just use the spores as they are to knock up a jar.



:whathesaid:
Unless you're trying to stretch them out into more syringes.

I PC my agar for 30M so to sterilize water 30M at pressure is sufficient.


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Offlineblojo02184
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Re: sterile container [Re: bodhisatta]
    #19350488 - 12/31/13 10:04 AM (10 years, 1 month ago)

I'm trying to expand the 5cc deep freeze syringe into 20cc syringe so as to stretch it.
30 minutes huh?

Container going in empty, then once sterile injecting the deep freeze, then sterile water. Shake, suck it up and knock some rye up.

Thanks guys


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InvisiblebodhisattaMDiscordReddit
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Re: sterile container [Re: blojo02184]
    #19350594 - 12/31/13 10:36 AM (10 years, 1 month ago)

Quote:

blojo02184 said:
I'm trying to expand the 5cc deep freeze syringe into 20cc syringe so as to stretch it.
30 minutes huh?

Container going in empty, then once sterile injecting the deep freeze, then sterile water. Shake, suck it up and knock some rye up.

Thanks guys




Sounds like a good time to experiment with agar.


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