|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
Only thing I really did different than most was use a spray bottle of 70% iso in place of Lysol thought we had some it was foaming bathroom cleaner. Eh
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
hooked! another win for g2g!
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19430750 - 01/16/14 10:09 PM (10 years, 14 days ago) |
|
|
Yea I think for now on my procedures for acquiring new specimens should go as follows  > Inoculate four small 1/3 full containers of WBS , > Each with slightly less than 1cc of spores per container.  > After the best container fully colonizes > This is more how it look , > Then I'd strip the mycelium for a full GLC syringe. Btw this is the syringe I revived! > After that recovers inoculate two master grain jars with the stripped the mycelium. > after all of the containers are fully colonized again/finally I'd G2G. > After they reach their 100% mark I would then spawn tem to bulk.  > Sound legit?
All these picture in this are my own. So ya I been there done that!
Edited by mister brown 420 (01/16/14 11:21 PM)
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
you could probably use the one small container to g2g to your 2 masters and skip the glc all together
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19430784 - 01/16/14 10:14 PM (10 years, 14 days ago) |
|
|
The point of the GLC was to put it in the fridge for when I'm out of spawn because its faster than MS.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
awesome give'r
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19431095 - 01/16/14 11:18 PM (10 years, 14 days ago) |
|
|
I added picturess to the procedure list for the visual learners out there... I'll admit I like threads with pictures too.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
I KNOW RIGHT! fucking frank won't add pics to his bacon tek, he's such a noob sometimes i was gonna do it for him today but i forgot got too excited and just pigged out
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19431125 - 01/16/14 11:23 PM (10 years, 14 days ago) |
|
|
I miss costa Rica's now I forgot how crazy those grew.. We shall meet again!
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19431142 - 01/16/14 11:26 PM (10 years, 14 days ago) |
|
|
Quote:
cronicr said: I KNOW RIGHT! fucking frank won't add pics to his bacon tek, he's such a noob sometimes i was gonna do it for him today but i forgot got too excited and just pigged out
Yea I just started taking pictures of All Most the steps rather than end results.. I'm trying to be interesting!
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
Shit gets you excited there is no stopping 
It's a bitch when you try to explain things with out pictures.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
|
i like what u did there with the small PP5's for a G2g. ALL of my bacteria now comes from G2G's, with the donor jar having clean growth from a clean agar wedge. i do 1 donor to 6-7 receivers, and the last 2 seem to get bacteria every time. my best guess it my SAB too small and to get the last bit of grain out, i have to maneuver so much in the SAB tilting both jars that the air moves too quick or something unwiped with iso is allowed to briefly pass above the donor jar. with what u have here, its easier because ur donor jar is smaller, thus easier to manipulate in the SAB.
i've actually considered doing this before that involved something like that (smaller donor jars essentially). my next experiment will be to use a spoon to transfer the grains because i can't find a clear tote tall enough for 2 qt jars to stand on top of one another. if that does not work, i will try what u have done here.
looking good MB 420!
p.s. I still use LC's hehehe... but only if i started from an agar wedge. it was another attempt to get around the G2G bacteria problem.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
Thanks leaf. I am glad I only had to do those 4 quart sized jars not much more space in my sab, how ever I have two SAB... lol
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
The G2G is recovering nicely going to wait another day or two to put pictures up of it! The white myc has fully re-covered the popcorn again and is starting to branch off into the rest of the WBS.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
 all my g2g is almost done and i'm getting ready for the next round, not gonna spawn anything till all my jars are done and i got a few more masters ready
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19440846 - 01/19/14 01:56 AM (10 years, 12 days ago) |
|
|
Your ballin'!! So that bob's red mill Worked out great all the glad mini rounds with straight mix or half that and half popcorn are growing great. I'm pretty excited.. Can't wait. I got two more g2g coming up in a few days!
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
|
i really need bags this jar shit gets old fast when your constantly doing them lol
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
I think its time to bring back my MVP A++ I got to revive a print of mine but.. I need some color variation and falbino the syringe I got has yet to provide me with myc.. I used the whole thing up, still no signs.. It's okay though I just won't ever buy that kind again. I would be willing to trade a albino a + print for Costa Rica if anyone has some
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
Re: liquid culture advice [Re: cronicr]
#19440861 - 01/19/14 02:02 AM (10 years, 12 days ago) |
|
|
Quote:
cronicr said: i really need bags this jar shit gets old fast when your constantly doing them lol
I was never interested in glass jars. I wouldn't mind having some bags to pasteurize my coir in but I've seen good results from just using gallon zip-lock bags on franks pasteurization thread.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
mister brown 420
perdido



Registered: 04/26/11
Posts: 800
Last seen: 9 years, 6 months
|
|
Well I know I said I wasn't going to take pics tonight but here is one.
-------------------- Estoy perdido y no me importa' deje que se vaya y que sea. hay amor. Last on 7/7/2014 See you space cowboy!
|
|