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OfflineMosey3012
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Re: Ps. Galindoi Spore germination on agar [Re: Gorlami]
    #19446154 - 01/20/14 05:49 AM (10 years, 11 days ago)

I had the same issue. While making spawn for another project I just threw in a couple hardly filled 1/2 pint jars of rye berries and scrapped some spores directly onto them. For what ever reason they had no issues germinating onto that... you'd think that it would be thriving on the agar. Been missing with different amount of agar/nutrients now so maybe that will solve the issue. Anyway, I let that grow out a bit then simply just scooped a grain up and threw that on agar...

Id still like to get it to germinate on agar tho, thinkin I may try some PDA or check around and see what others are having success on. MEA has worked well for me but its always spores that give me a hit or miss on germination. Best of luck!


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Offlinegornyhuy
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Registered: 08/04/09
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Re: Ps. Galindoi Spore germination on agar [Re: Gorlami]
    #19446699 - 01/20/14 09:01 AM (10 years, 11 days ago)

Quote:

whalechunks said:
Quote:

blueconfusion said:
It does but remember honey is a preservative, I have never used it myself in agar, but I have seen it used in other recipes.  I've used it in LCs but like I said it is a preservative so use sparingly.





Thanks, that may have been the problem in the end.  That or crappy technique.

I made another agar batch but with Dextrose this time, spores germinated in 2 days. Wowzers! :laugh:

Thanks to everyone who chimed in, couldn't ask for more! :smile:




Thanks for the update!  I have just gotten a big bag of LME and am about to make my first dextrose based agar and probably LC while I'm at it.  Very interested to see how it performs versus the old potato + honey recipe.


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