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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Do these jars look ready to spawn?
#19345987 - 12/30/13 11:55 AM (10 years, 1 month ago) |
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Hey folks,
I'm in no real rush, but have some spare time today that i could use towards setting up my first tub. I have 4 qts of spawn That looks like it is very close to being ready. There are still a few areas pressing up against the glass that have not colonized.
Pics included. Should I give it a few days? Or should I begin to pasteurize my coir and verm?

-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346000 - 12/30/13 11:59 AM (10 years, 1 month ago) |
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start pasturizing
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346001 - 12/30/13 12:00 PM (10 years, 1 month ago) |
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Get pasteurizing. Those small spots against the glass probably won't fill in.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: Do these jars look ready to spawn? [Re: Pastywhyte]
#19346021 - 12/30/13 12:07 PM (10 years, 1 month ago) |
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Thanks fellas!
Also, I'm having an issue with RGS. Should I start a seperate thread? I don't want to clog up forum space, and I've seen you guys both offering great advice for folks.
Basically, no matter what I do to sterilize the RGS, it is contaminating.
PC at 15psi for 120mins, same "sterile" technique when innoculating the jars as the WBS, but each jar contaminates.
I'm at a loss.... coincidence?
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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DebuteMachine

Registered: 09/29/06
Posts: 6,457
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Re: Do these jars look ready to spawn? [Re: Pastywhyte]
#19346023 - 12/30/13 12:08 PM (10 years, 1 month ago) |
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Those look...
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346033 - 12/30/13 12:13 PM (10 years, 1 month ago) |
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Quote:
MarcusFreeman said: PC at 15psi for 120mins, same "sterile" technique when innoculating the jars as the WBS, but each jar contaminates.
Probably your inoculate if your sterile tek is up to snuff. Are you using ms syringes? What kind of contamination, bacteria, mold? More details of your procedures and some pics would help.
Even now when I use ms solution to inoculate grains (which ain't that often), I expect to loose a jar or two and then G2G the ones that make it. Agar is the only inoculate that can be guaranteed clean.
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Citric


Registered: 03/19/04
Posts: 4,490
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Re: Do these jars look ready to spawn? [Re: Pastywhyte]
#19346053 - 12/30/13 12:21 PM (10 years, 1 month ago) |
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I had issues with Rye as well, no matter how it was prepped or sterilized it always led to bacteria / wet spot. Few others have had this issue and chalked it up as a bad batch of grain. I never went back to Rye, specially seeing how WBS is king for spawning anyhow.
Those jars are ready as well.
-------------------- Self Healing lid tek ** Update 10.17.17 ** Mini casing pictures: Pins to harvest Cup O' Shrooms Magash: I noticed my contams were in the shape of fingers Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger. My final Grow!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Do these jars look ready to spawn? [Re: Citric]
#19346064 - 12/30/13 12:25 PM (10 years, 1 month ago) |
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yeah i tried rgs a couple times and it was a 50/50 for me, i need to read more about it i'm sure i'm going wrong in the hydrating part but i like my wbs
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Do these jars look ready to spawn? [Re: cronicr]
#19346089 - 12/30/13 12:31 PM (10 years, 1 month ago) |
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I have not tried RGS yet (gonna soon tho), but rye berries have been very kind to me If I could find cheap WBS that wasn't full of peanuts, rasins, gum, cracked corn, nails, and other assorted crap, then I would use that. But so far the only WBS that I have seen that fits that bill in my area, costs almost as much as my rye.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: Do these jars look ready to spawn? [Re: Citric]
#19346137 - 12/30/13 12:41 PM (10 years, 1 month ago) |
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I have made like5 seperate batches using the same RGS, prepping like RR reccomends, Quote:
Citric said: I had issues with Rye as well, no matter how it was prepped or sterilized it always led to bacteria / wet spot. Few others have had this issue and chalked it up as a bad batch of grain. I never went back to Rye, specially seeing how WBS is king for spawning anyhow..
^^^^ This. It is primarily bacterial. I think there was maybe one jar that had trich, but I think that one was my error.
I did multispore for my first grow, and since then have been using agar- as I have not gone back to PF cakes, and agar is super easy.
I was starting to wonder if it was the bag of seed or my procedures.
I made 6 jar of substrate this past attempt. 4 wbs, 1 50%wbs/50%rgs, and 1 75%wbs/25%rgs.
The 4 that survived were the 100%wbs. I decided to mix in the RGS with the WBS so that I could have some sort of comparison.
I was beginning to get frustrated at myself.
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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Citric


Registered: 03/19/04
Posts: 4,490
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346162 - 12/30/13 12:47 PM (10 years, 1 month ago) |
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Quote:
MarcusFreeman said: I have made like5 seperate batches using the same RGS, prepping like RR reccomends, Quote:
Citric said: I had issues with Rye as well, no matter how it was prepped or sterilized it always led to bacteria / wet spot. Few others have had this issue and chalked it up as a bad batch of grain. I never went back to Rye, specially seeing how WBS is king for spawning anyhow..
^^^^ This. It is primarily bacterial. I think there was maybe one jar that had trich, but I think that one was my error.
I did multispore for my first grow, and since then have been using agar- as I have not gone back to PF cakes, and agar is super easy.
I was starting to wonder if it was the bag of seed or my procedures.
I made 6 jar of substrate this past attempt. 4 wbs, 1 50%wbs/50%rgs, and 1 75%wbs/25%rgs.
The 4 that survived were the 100%wbs. I decided to mix in the RGS with the WBS so that I could have some sort of comparison.
I was beginning to get frustrated at myself.
Sterilize some jars and just leave them, don't even inoculate. When I tried this, they still contaminated. *shrug*. Go with WBS for spawning
-------------------- Self Healing lid tek ** Update 10.17.17 ** Mini casing pictures: Pins to harvest Cup O' Shrooms Magash: I noticed my contams were in the shape of fingers Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger. My final Grow!
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346171 - 12/30/13 12:49 PM (10 years, 1 month ago) |
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Quote:
MarcusFreeman said: PC at 15psi for 120mins, same "sterile" technique when innoculating the jars as the WBS, but each jar contaminates.
Sounds Perfect.
Now to test your jars versus your culture. Pressure cook as always, and leave one or two jars off to the side uninoculated. This will help you to verify if your problem is with the culture, or your jar.
My opinion on this:
Sounds like your jars are sterilized. How/where do you inoculate. Do you use a still air box or flow hood? Looks like you are using polyfill, which if not tight enough could cause issues. Do you inoculate through the poly? No MSS is totally clean, so problems could be there
These are the variables that I can think of that could cause you issues.
If the jars you set off to the side go bad, then you know you have issues with contaminants getting in somehow....I highly doubt that if a jar of grain that spent 2 hours @ 15 psi could allow for any survivors.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346188 - 12/30/13 12:52 PM (10 years, 1 month ago) |
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Here is the 50/50 after shaking. It was fucked before shaking, but I was curious what would happen. I wish I had taken a pic a few days ago, before the shake.
In the beginning, it liked like out was going well. After about a week, the others were looking nice, but the 2 with RGS had the nasty brown goo mixed in.

I used one disk to inoculate the 6 jars, and also 2 new dishes. The agar looks ok to me, but I'm still a noob. Am I missing anything?

That's a good idea Citric, I will do that. I probably should have done that already. Lol
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Quote:
Whippy said:
Quote:
MarcusFreeman said: PC at 15psi for 120mins, same "sterile" technique when innoculating the jars as the WBS, but each jar contaminates.
Sounds Perfect.
Now to test your jars versus your culture. Pressure cook as always, and leave one or two jars off to the side uninoculated. This will help you to verify if your problem is with the culture, or your jar.
My opinion on this:
Sounds like your jars are sterilized. How/where do you inoculate. Do you use a still air box or flow hood? Looks like you are using polyfill, which if not tight enough could cause issues. Do you inoculate through the poly? No MSS is totally clean, so problems could be there
These are the variables that I can think of that could cause you issues.
If the jars you set off to the side go bad, then you know you have issues with contaminants getting in somehow....I highly doubt that if a jar of grain that spent 2 hours @ 15 psi could allow for any survivors.
For these, I have been using agar transfers in a still air box.
To prep the still air box, I spray with bleach/water, wipe down, let dry, then spray with 90%rubbing alcohol before working in there. I make sure the sides are wet so that air born contams will get stuck in the alcohol if they touch it.
I use gloves and frequently spray with alcohol if I take my hands out of the box. I try to move slowly so the air doesn't move rapidly and kick up contams.
I use medical grade scalpels that I PC for 20 mins before use(wrapped in foil) before working with the agar.
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346240 - 12/30/13 01:06 PM (10 years, 1 month ago) |
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Whippy, I am primarily using polyfill, but have used tyvek. I did use tyvek on one of the contaminated jars(another variable). The other contaminated jar had polyfill.
I was told that polyfill is superior to tyvek, but it was free at the post office, so decided to give it a shot.
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346269 - 12/30/13 01:14 PM (10 years, 1 month ago) |
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Quote:
MarcusFreeman said: Whippy, I am primarily using polyfill, but have used tyvek. I did use tyvek on one of the contaminated jars(another variable). The other contaminated jar had polyfill.
I was told that polyfill is superior to tyvek, but it was free at the post office, so decided to give it a shot.
Sounds either like the culture, or the method of introducing the culture to the grain.
Shut off all types of fans and air movers. This includes computer fans etc.
I lightly spray my sab with 1/10 bleach, than put in my work pieces. Mist the lid, then put a nice mist into the air above the box, then shut the lid for an hour or more. I use tyvex squares to cover the arm holes and I tape them down so that no air can disturb.
I try to give myself about an hour prior to work. I don't use any lysol ever. I only use 70% iso to wash my gloves, and then I flame my scalpel...which usually is floating in 70% iso alcohol.
My "franken-sab" used to be a 106qt monotub, so the holes are still there, just taped over. Its about as ghetto as I have seen around these parts, but works splendid.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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You gotta look close at your cultures before using them to inoculate. Many times the bacteria can be very hard to spot on the agar as they can be really close in color and I lost a few masters this way when I was first starting with agar. Now I color my agar with food coloring to make any contams stand out.
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MarcusFreeman



Registered: 09/16/13
Posts: 376
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Re: Do these jars look ready to spawn? [Re: Pastywhyte]
#19346327 - 12/30/13 01:34 PM (10 years, 1 month ago) |
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Thanks for the responses, guys. I won't give up on RGS just yet(because I have a big ass bag of it).
I'm going to take the blame on this one and assume that I still need to refine my procedures.
I'm still going to test a few contol jars of the RGS, just to know. Im thinking I'll leave one unopened, and the other I will open and close quickly, inside the SAB.
-------------------- "The trick is to use the drugs once to get there, and maybe spend the next ten years trying to get back there without the drug." MJK As one ends, another begins.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Do these jars look ready to spawn? [Re: Pastywhyte]
#19346444 - 12/30/13 02:01 PM (10 years, 1 month ago) |
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Quote:
Pastywhyte said: You gotta look close at your cultures before using them to inoculate. Many times the bacteria can be very hard to spot on the agar as they can be really close in color and I lost a few masters this way when I was first starting with agar. Now I color my agar with food coloring to make any contams stand out.
I checked out your agar tek yesterday. last night I bought a ton of pp5s and some food coloring. I went with a dark purple colore that looks like it will show growth very well. Great idea man.
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blojo02184
Big Red



Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 13 days, 5 hours
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Re: Do these jars look ready to spawn? [Re: MarcusFreeman]
#19346652 - 12/30/13 02:50 PM (10 years, 1 month ago) |
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Quote:
MarcusFreeman said: Whippy, I am primarily using polyfill, but have used tyvek. I did use tyvek on one of the contaminated jars(another variable). The other contaminated jar had polyfill.
I was told that polyfill is superior to tyvek, but it was free at the post office, so decided to give it a shot.
Sfd man... Sfd all the way! Check out sponsors, or search for cellulose acetate filter disk with a micron rating of .2
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