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InvisibleMudaFuka
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can i store tissue for cloning
    #19338824 - 12/28/13 08:46 PM (10 years, 1 month ago)

I kick puppies


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AMU
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Edited by cronicr (01/02/16 04:59 PM)


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OfflineMr.Sir
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Re: can i store tissue for cloning [Re: MudaFuka]
    #19338844 - 12/28/13 08:50 PM (10 years, 1 month ago)

I believe it is do-able with fresh-ish fruits, but would certainly be best the fresher you can get it. Pins/aborts will do great on agar as their growth rate is exceptional and can often outrun contams. good luck


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InvisibleMudaFuka
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Re: can i store tissue for cloning [Re: Mr.Sir]
    #19338927 - 12/28/13 09:06 PM (10 years, 1 month ago)

http://
iv still got a number of interesting fruits growing on my sub, not quite as big as my first two candidates but still not bad. should I just wait and use one of them.


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OfflinecronicrFacebook
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Re: can i store tissue for cloning [Re: MudaFuka]
    #19338941 - 12/28/13 09:08 PM (10 years, 1 month ago)

it will be fine till it cools, try a couple or more have fun with it:thumbup:


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InvisibleMudaFuka
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Re: can i store tissue for cloning [Re: cronicr]
    #19338981 - 12/28/13 09:16 PM (10 years, 1 month ago)

id love to try more but iv only got 8 pp5s available at the moment. my bacterial contam rate has been insane lately so I want to take a few clones from each fruit.


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Invisibleuncle_rico
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Re: can i store tissue for cloning [Re: MudaFuka]
    #19339120 - 12/28/13 09:45 PM (10 years, 1 month ago)

Cloneufc had a clone tek where the tissue sample was placed in a zip-lock baggie for a few days to recover before it was placed on agar.  perhaps this will buy you the time you need.

http://www.shroomery.org/forums/showflat.php/Number/12032286#12032286


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InvisibleMudaFuka
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Re: can i store tissue for cloning [Re: uncle_rico]
    #19339172 - 12/28/13 10:01 PM (10 years, 1 month ago)

it's fine my agar cooled faster than I thought it would. iv already taken 2 clones from each fruit. in the morning I will take 4 more from different fruits.

I hope my extra long PC time (75mins) will help with my contam rate.


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