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Offlinelonelyjew
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Registered: 05/02/13
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grain jars colonizing slowly... but still colonizing
    #19120408 - 11/11/13 12:45 PM (10 years, 3 months ago)

Post deleted by lonelyjew

Reason for deletion: Just causesad:


Edited by lonelyjew (08/09/16 05:43 AM)


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OfflinePussyFart
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19120437 - 11/11/13 12:50 PM (10 years, 3 months ago)

How many jars did you inoculate with the 2 syringes?

Might just be too much moisture.

Pics?


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: PussyFart]
    #19120464 - 11/11/13 12:55 PM (10 years, 3 months ago)

Post deleted by lonelyjew

Reason for deletion: Just cause


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Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


Edited by lonelyjew (08/09/16 06:19 AM)


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OfflineSgt. Pepper
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19120561 - 11/11/13 01:13 PM (10 years, 3 months ago)

I have colonization start on the bottom all the time and I'm very conservative with my spores/lc. You may have a bacterial contamination.


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: Sgt. Pepper]
    #19120580 - 11/11/13 01:17 PM (10 years, 3 months ago)

damn that sucks, think i should just let them sit for another few weeks? couldn't really hurt could it?


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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OfflinePussyFart
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19120589 - 11/11/13 01:19 PM (10 years, 3 months ago)

They should have been fully colonized, or close to it, by 3 weeks in.

I would start over, using less spore solution(only a few drops per hole).

You could try shaking the grain jars you got, and see if in a week they look better.


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: PussyFart]
    #19120653 - 11/11/13 01:35 PM (10 years, 3 months ago)

alright yeah i guess i'll shake them     



i only have 1 cc of spore solution left so hopefully i get a good grain jar and can make a grain lc out of it!!


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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OfflinePussyFart
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19120694 - 11/11/13 01:44 PM (10 years, 3 months ago)

Quote:

lonelyjew said:
i only have 1 cc of spore solution left so hopefully i get a good grain jar and can make a grain lc out of it!!



I would just G2G the jar out....

Don't even think about making a LC unless you plan on starting it from an agar wedge.

Just trying to save you from failure.

After messing with agar and G2G transfers, it is quite possible that you will never touch a needle again.


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: PussyFart]
    #19120703 - 11/11/13 01:47 PM (10 years, 3 months ago)

hmmm i guess it couldn't hurt to try grain to grain for some reason it just makes me nervous.


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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OfflinePussyFart
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19120724 - 11/11/13 01:52 PM (10 years, 3 months ago)

Do it in a SAB or in front of a flow hood and there is nothing to be nervous about.

The jars will be open for less than 3 seconds in a very clean environment.


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: PussyFart]
    #19120733 - 11/11/13 01:55 PM (10 years, 3 months ago)

yeah i guess your right well i got a SAB maybe i could try it on my oyster jars


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19127334 - 11/12/13 04:39 PM (10 years, 3 months ago)

alright well they are recovering from the shake just really slow


so does that mean i should just leave them alone?


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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OfflinePussyFart
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19127486 - 11/12/13 05:04 PM (10 years, 3 months ago)

Yes.


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Offlinemthawkins
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Re: grain jars colonizing slowly... but still colonizing [Re: PussyFart]
    #19130790 - 11/13/13 11:18 AM (10 years, 3 months ago)

Leave them alone. However if it's bacteria contam from the moisture then there's a chance that will overgrow the myc.. talking from experience lol.
Keep eye on them and update us!


--------------------
“Never laugh at live dragons.”
- J.R.R Tolkien


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing [Re: mthawkins]
    #19132043 - 11/13/13 04:29 PM (10 years, 3 months ago)

well the myc is still definitely growing but at like half a grain a day maybe it will speed up as it drys out?(or just contam) if that LC i made (i know i know) works then i will just start over and inoculate with that.





Edit: LC was made a week or two prior wanted to make that clear


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


Edited by lonelyjew (11/13/13 05:43 PM)


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OfflineBittrBuffalo
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19137376 - 11/14/13 05:59 PM (10 years, 3 months ago)

There's probably just too much water in your jars.:frown: I had that problem recently. I had previously used a the Doc's tek for WBS, a hybrid of that and RR's tek for ORB substituting WBS, then I tried FrankHorrigan's (:heart: you FrankHorrigan). The most successful for me was with the RR blend (:heart: you again, FrankHorrigan). The secret, I've found, to keep WBS from going too sticky after you simmer it is to change the water when you soak at least once, then rinse it in hot water, mixing it around with your hands to remove the extra starch when you strain it. You have to make sure it's nice and dry (do the TP test).

So this time around I didn't fully simmer the WBS because I didn't want the exploded kernel issue. I did let Mr. Buffalo (who has very little interest in everything involved prior to harvest time) drain and pack the jars, PC, and inoculate for me. He said it passed the TP test, but maybe there was too much liquid from the syringes that wasn't absorbed by the grain. I dunno. Anyway. The point is, there was too much liquid in there and it's been over 4 weeks and we're only at about 60-70%. It's GT, which is slow to colonize anyway, and it's getting cold in the mountains. So this is what I've done to speed things along: put the jars in a warmer room, gently shaken the jars to keep the water from pooling (without breaking up the mycelium), then set it up under the fan from the dehydrator to incubate it. This seems to be making a significant difference. It should start to speed up if you keep it warm. It could be the water evaporating off the grain and condensing on the glass, or the mycelium liking the heat. Whatever it is, it's working. If yours stalls out completely, then you probably have bacterial contam. You can tell because it'll smell like barf.

ANYWAY...if it's any consolation, PrimalSoup is doing a potency experiment in which he postulates that slower colonization/longer consolidation times directly correlate to greater potency. Here ya go:

The Potency Project

Happy growing! :mushroom2:


--------------------
Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.:mushroom2:


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Offlinelonelyjew
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Registered: 05/02/13
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Re: grain jars colonizing slowly... but still colonizing [Re: BittrBuffalo]
    #19137947 - 11/14/13 07:46 PM (10 years, 3 months ago)

hmm alright i will definitely put these ones in a warmer area and i know they definitely sped up after i shook them.


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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Offlinelonelyjew
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Re: grain jars colonizing slowly... but still colonizing *DELETED* [Re: lonelyjew]
    #19338446 - 12/28/13 07:38 PM (10 years, 1 month ago)

Post deleted by lonelyjew

Reason for deletion: Just cause


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


Edited by lonelyjew (08/09/16 06:20 AM)


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OfflineMaJiK_420
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Re: grain jars colonizing slowly... but still colonizing [Re: lonelyjew]
    #19338628 - 12/28/13 08:10 PM (10 years, 1 month ago)

Quote:

lonelyjew said:
update/what the hell is up with my grain jars?

so its been 68 days total since i inoculated them and they are right around 97%-98% still colonizing but still slow


what the hell is wrong with the jars?


i had oyster colonize WBS jars made the exact same way in under ten days


is there any grain that is easier i tried winter wheat berries but its the same thing i guess i could try millet but at this point i don't think it will really be that much better.



is the fact that they are pint jars instead of quart making all the difference?


im assuming that it was just the moisture content but soaking them longer didn't seem to make a difference neither did loading them up longer

it took 2 weeks to see growth from a water LC!!!(made from a brf cake still in jar with SHIP and filter so it was clean)


any ideas?

been wanting to do a monotub but at this point it would seem the only way i could do it is to make a lot of brf jars but that seems pretty time consuming.


(didn't know whether to make a new thread i figured i would just resurrect my old one since its about the same jars)





I have run into similar problems a couple times now.

I think its a combination of a few different factors/variables.

First, not all myc is equal. Some grows fast, some grows slow. since I started doing some agar work this becomes really obvious. Some strains boom, while others just kinda ho hum and smell the roses and shit. Genetics is the first factor I think.

Second I think moisture content/moisture retention. What kind of lids are you using my friend? if polyfill, then maybe try adjusting how tightly packed it is. I think one of my SUPER slow jars (about 3 months to get 50%) the poly was a little loose and I think the seed dried out some in that time. And then birdseed being kinda tricky to properly hydrate in the first place.

So I wouldn't give up hope, but I might just try again fresh if possible. I just found a jar that I shot up a month or two ago, waiting for contam or good stuff.... Nothing! Until yesterday I turned it around and found a nice cookie sized myc patch. Looks like some slow stuff, but if you can transfer to agar or g2g it might get a second wind and take off if the conditions change slightly to favor those individual genetics.

Just a bunch of trial and error to find the good stuff. Thats another reason good to learn agar cuz then you should be able to isolate the good fast strains and have more consistent results.


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