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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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How long do you let your needle cool for after flame sterilizing?
#19338622 - 12/28/13 08:08 PM (10 years, 1 month ago) |
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I'm curious because on the one hand I don't want to scorch the spores especially if I'm just doing one drop for agar work. On the other hand the longer I wait the more likely it is that contams will land on it. I suppose the only way to really know is to take a laser thermometer and point it at the needle. Has anyone done this?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: How long do you let your needle cool for after flame sterilizing? [Re: invitro]
#19338627 - 12/28/13 08:10 PM (10 years, 1 month ago) |
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just squirt a couple drops in your sab or dip it in some agar or wait
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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EastBayRay

Registered: 06/06/13
Posts: 746
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Re: How long do you let your needle cool for after flame sterilizing? [Re: cronicr]
#19338657 - 12/28/13 08:15 PM (10 years, 1 month ago) |
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I apply red rtv to the 'bottom side' (the part inside the jar) of red gaskets and use those as my inoculation ports. When the rtv has cured after application (up to 24 hours) you can inoculate.
I flame sterilize, and while still red hot, I press the needle through the port and then let it cool by squirting liquid through the needle. Easy peasie.
After some time you will need to apply new rtv but it takes a while if you did it correctly the first time you applied it.
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: How long do you let your needle cool for after flame sterilizing? [Re: cronicr]
#19338908 - 12/28/13 09:02 PM (10 years, 1 month ago) |
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Quote:
cronicr said: just squirt a couple drops in your sab or dip it in some agar or wait
I agree I squirt a few drops then take the last drip left and touch the agar. If you have problems with that then cool in the receiving agar put a drop. Sterilize repeat. Try them all pick what works best for you.
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ringzero
Stranger

Registered: 03/04/13
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Re: How long do you let your needle cool for after flame sterilizing? *DELETED* [Re: magickspore] 1
#19339246 - 12/28/13 10:19 PM (10 years, 1 month ago) |
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Post deleted by ringzeroReason for deletion: v
Edited by ringzero (12/28/13 10:21 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: How long do you let your needle cool for after flame sterilizing? [Re: ringzero]
#19340441 - 12/29/13 06:39 AM (10 years, 1 month ago) |
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Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower.
RR
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bchighway


Registered: 12/03/13
Posts: 60
Loc: Canada
Last seen: 9 years, 5 months
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Re: How long do you let your needle cool for after flame sterilizing? [Re: PussyFart]
#19340500 - 12/29/13 07:03 AM (10 years, 1 month ago) |
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Quote:
Notahacker420 said: Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower.
RR
o'come on...
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: How long do you let your needle cool for after flame sterilizing? [Re: PussyFart]
#19340871 - 12/29/13 10:06 AM (10 years, 1 month ago) |
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Well most people use alcohol to wipe a ship before inoculation. Should I be using iodine instead? What kinds of contams can live in 70% alcohol, do you have any links to references?
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retaardvark
Stranger

Registered: 12/22/12
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Re: How long do you let your needle cool for after flame sterilizing? [Re: invitro]
#19340889 - 12/29/13 10:13 AM (10 years, 1 month ago) |
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Quote:
invitro said: Well most people use alcohol to wipe a ship before inoculation. Should I be using iodine instead? What kinds of contams can live in 70% alcohol, do you have any links to references?
He was only speaking of tools you can flame sterilize. Alcohol on a clean paper towel is fine to wipe off an injection port
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: How long do you let your needle cool for after flame sterilizing? [Re: invitro]
#19340901 - 12/29/13 10:16 AM (10 years, 1 month ago) |
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We wipe the SHIP with alcohol because we cannot flame sterilize it.
Alcohol does not kill mold spores on contact.....fire does.....
If we kill everything on the needle with fire and then wipe it with alcohol it then becomes unsterile.
Wiping the tools that cannot be flame sterilized with 70% alcohol is common practice, as it does sanitize.....but you could possible be wiping mold spores over everything.
Just expunge a few drops of spore solution to cool the needle after flaming, then the rest will flow contam free.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Gr13nMushDude
Stranger


Registered: 05/25/13
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Re: How long do you let your needle cool for after flame sterilizing? [Re: PussyFart]
#19340932 - 12/29/13 10:26 AM (10 years, 1 month ago) |
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Good to know I've been doing it right not that I plan on using syringes much more. Agar boat here I am.
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