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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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Re: Think I might have sterilized my sub [Re: cronicr]
#19279881 - 12/15/13 03:28 PM (10 years, 2 months ago) |
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Good stuff, SpitballJedi!
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   The Corbett Report Open Source Intelligence News
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: Pestile]
#19312020 - 12/22/13 02:01 PM (10 years, 2 months ago) |
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Quote:
SpitballJedi said: I just put my sterilized CVG WBS sub in to fruiting.
13 days of colonizing. I could have put it in to fruiting a couple of days ago, but life held me back.

is life done holding you back? hows this coming along?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Think I might have sterilized my sub [Re: cronicr]
#19316313 - 12/23/13 01:58 PM (10 years, 2 months ago) |
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I started seeing pins after 7 days. I got pneumonia and let it go. I'm just now starting to come back to reality.
The sterilizing seems to be working. results aren't impressive, but there's no contams still, which was really the point of the experiment.
I know it's only one experiment, but it seems to be a wash for me. I'll probably stick with proper pasteurization because it's just a good habit. But, if someone finds sterilizing CVG to be easier than pasteurization, then I say go for it.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: SpitballJedi]
#19316331 - 12/23/13 02:02 PM (10 years, 2 months ago) |
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holy fuck, well glad your getting better man thats not something to fuck around with
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Think I might have sterilized my sub [Re: SpitballJedi]
#19316347 - 12/23/13 02:06 PM (10 years, 2 months ago) |
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Quote:
SpitballJedi said: I started seeing pins after 7 days. I got pneumonia and let it go. I'm just now starting to come back to reality.
That's horrible man, I had pneumonia once, terrible shit. Hope your feeling better soon.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Think I might have sterilized my sub [Re: Pastywhyte]
#19335143 - 12/28/13 12:29 AM (10 years, 1 month ago) |
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Well this little experiment is now over. Tub #3 put out a fourth flush then contamed, tub #4 put out its fourth flush, no contams, but I pitched it anyway, need the space. Since starting these tubs I have done a couple of trays with sterilized coir verm and just got my second flush from them. I now have several tubs going with sterilized coir and verm, I think they will do well.
Might still post some results here for some of my other tubs, but I think its safe to say that this practice has some merit and may present a simpler means of substrate prep than stovetop pasteurization. If anyone has sterilization results to share, please feel free to do so here.
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bluecap
mychanical



Registered: 11/10/13
Posts: 286
Last seen: 8 years, 5 months
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Re: Think I might have sterilized my sub [Re: Pastywhyte]
#19335862 - 12/28/13 07:30 AM (10 years, 1 month ago) |
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good experiment man. I,ve had good results, using sterilized coir/verm. been doing this for a few months now. subs fruit great, and low contam rates, until 4th or fifth flush. which I think would occur anyway, even if using pasteurization method. I sterilize my bulk substrates, in mycobags with the filter patch for 60 minutes. no probs here...I love using coir!...
-------------------- I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma... 
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: bluecap]
#19336907 - 12/28/13 12:34 PM (10 years, 1 month ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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sololas
Feel like a Stranger



Registered: 12/26/13
Posts: 476
Loc: Atlantis
Last seen: 1 year, 11 months
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Re: Think I might have sterilized my sub [Re: cronicr]
#19338463 - 12/28/13 07:41 PM (10 years, 1 month ago) |
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Seeing this just made my month, was debating what sub and if I was going to sterilize or pasterize. Well I have both coir and verm so this thread caught my eye being its fresh on here. Seems coir is a great tool in mycology if prepared properly for the task. Next time im gonna throw in some dried hpoo from a friends stable.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: sololas]
#19338473 - 12/28/13 07:43 PM (10 years, 1 month ago) |
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NO! you can get away with cvg but sterile poo is asking for trouble
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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sololas
Feel like a Stranger



Registered: 12/26/13
Posts: 476
Loc: Atlantis
Last seen: 1 year, 11 months
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Re: Think I might have sterilized my sub [Re: cronicr]
#19338558 - 12/28/13 07:59 PM (10 years, 1 month ago) |
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Its fully dried and I would try and pasterize the hpoo not sterilize. And I would isolate the tub in a different area. Just something I've wanted to tackle the hpoo thing cause its free and close. Im just impressed with the results they got from cvg I didnt think it would put out 4 strong flushes.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: sololas]
#19338565 - 12/28/13 08:00 PM (10 years, 1 month ago) |
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gotcha! just making sure h-poo is awesome!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Think I might have sterilized my sub [Re: cronicr]
#19338570 - 12/28/13 08:01 PM (10 years, 1 month ago) |
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2 weeks ago I spawned to bulk 5 qt of a clone to sterilized coir/verm.
My method:
Gallon+pint of water @ 212f Added to brick of coir and 2 qt verm double trash bagged inside of a small igloo cooler. After 4 hours temps were still over 180f, and I pulled the mass out and let it cool.
I did not report back because frankly I was superstitious. The clone that I had gotten has shown to be the slowest thing I have ever worked with. PE MS moves faster on grain than this clone.
It has been 15 days since I spawned, and I would say that today I hit the 100% mark (close enough for what I care).
I got to the point with this culture that I simply don't care if it fails.....But back OT, seems that sterilized coir/verm works splendid! Would be nice to have more try out this method, because it is simple to sterilize.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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i would like to see a monoculture comparison from pasturized to sterilized to see the diffrence in colonizing times and performance
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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sololas
Feel like a Stranger



Registered: 12/26/13
Posts: 476
Loc: Atlantis
Last seen: 1 year, 11 months
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Re: Think I might have sterilized my sub [Re: cronicr]
#19345677 - 12/30/13 10:08 AM (10 years, 1 month ago) |
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Quote:
cronicr said: i would like to see a monoculture comparison from pasturized to sterilized to see the diffrence in colonizing times and performance
Im thinking thats what ill do after my one tub finishes, I will be sure to startup a post and try one with poo, other sterile cvg got Two rooms for colonizing only one FC. Its so i can try different methods in grains, subs, etc. Makes me feel better so that what counts.... About a month and ill start the expeiment of faster colonizing times between the 2....
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Think I might have sterilized my sub [Re: sololas]
#19345883 - 12/30/13 11:22 AM (10 years, 1 month ago) |
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I might give a shot at a side by side with pasteurized CV vs sterilized CV. Would be interesting for sure to see if there are any + or -
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sololas
Feel like a Stranger



Registered: 12/26/13
Posts: 476
Loc: Atlantis
Last seen: 1 year, 11 months
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Re: Think I might have sterilized my sub [Re: Pastywhyte]
#19346092 - 12/30/13 12:32 PM (10 years, 1 month ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: sololas]
#19472496 - 01/25/14 02:51 PM (10 years, 30 days ago) |
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well i dunked my grains and spawned to strait sterilized coir, no verm in town but it was colonized in 5 days, just a tray with 2 qt's of JAS to 3 qt's coir. not sure if i wanna fruit it or case it first since it's only been 6 days
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
Edited by cronicr (01/31/14 02:37 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Think I might have sterilized my sub [Re: cronicr]
#19473129 - 01/25/14 05:35 PM (10 years, 30 days ago) |
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You should late case it with some sterilized coir as well
Edited by Pastywhyte (01/25/14 06:30 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Think I might have sterilized my sub [Re: Pastywhyte]
#19474162 - 01/25/14 09:41 PM (10 years, 30 days ago) |
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Quote:
Pastywhyte said:

You should late case it with some sterilized coir as well 
genius! i have some sterilized already, can't believe i didn't think about that lol
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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