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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Just Got a Scope! My First Sections... [Re: Byrain]
#19332392 - 12/27/13 10:10 AM (10 years, 1 month ago) |
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I have compared, briefly, manual measurements with another program (Macnification), and the manual software measurements I took were very close to the reticle measurements.
When you look through a reticle for the measurements, there is still a bit of mental judgment used to determine the amount of microns to the .5 or to the 1/10th micron. It's more precise if calibration and slide preparation are performed perfectly and software is used.
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Just Got a Scope! My First Sections... [Re: The Lightning]
#19332407 - 12/27/13 10:14 AM (10 years, 1 month ago) |
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Measurements don't need to be that accurate, off by .5 would still give you the same results. Remember you're comparing with people who did all the measurements by hand using these same mental judgments. 
These were spores you compared? How big were they? How about cystidia? Smaller spores often appear in their entirety without changing the focus.
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Just Got a Scope! My First Sections... [Re: Byrain]
#19332577 - 12/27/13 11:06 AM (10 years, 1 month ago) |
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Quote:
Byrain said: These were spores you compared? How big were they? How about cystidia? Smaller spores often appear in their entirety without changing the focus.
I can't recall but I believe I compared both cystidia and spores, and I'm unsure of the measurement sizes.
Super thin sections (and super thin spore slides with a very small amount of water) should improve the plain-level of sight and reduce the need for constantly re-focusing.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,312
Last seen: 3 days, 6 hours
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19332813 - 12/27/13 12:27 PM (10 years, 1 month ago) |
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Quote:
Hashfinger said: So yes because I am shooting down the lens with my camera manually, should I just go with the stage micrometer and reticle? Sounds like this is the more time-consuming, yet sure-fire way to get an accurate measurement.
As long as the camera lens is always the same distance from the eyepiece, I think you should measure digitally. Also helps to use the manual focus set at the same distance, as the focus can change the scale a bit. I usually set manual focus to about a meter.
Quote:
The Lightning said: Super thin sections (and super thin spore slides with a very small amount of water) should improve the plain-level of sight and reduce the need for constantly re-focusing.
Also pressing down firmly on the cover slip with some lens paper or paper towel helps force all the cells into the same focal plane.
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Lepkaun
Super Stooper



Registered: 10/07/10
Posts: 2,093
Loc: Close and Far
Last seen: 8 years, 4 months
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That's fucking awesome Hash!! I bet you were like when you got that bitch.
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Leppy say's: Seek and you will find!Ask and they will guide you!Listen and be taught!!
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Just Got a Scope! My First Sections... [Re: inski]
#19333409 - 12/27/13 03:33 PM (10 years, 1 month ago) |
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Quote:
inski said:
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maynardjameskeenan said:
Welcome to the club man! I'm still not very good at using this thing even though I got it a year ago. Practice makes permanent, so keep it up. I'm happy for you, merry Christmas  I just got a stage micrometer for Christmas this year, so I'll actually can measure the things that I see.
Nice, I have one too, you can't actually measure anything with it, you only use it to calibrate a reticle that is fitted into one of the eyepieces!
So either way I have to buy something else to get measurements? Either an eyepiece reticle or USB microscope camera?
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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RuralAnomaly
Sporadic


Registered: 10/05/13
Posts: 2,153
Loc: Spitzenkörper Ohio
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no, if you make a 'jig' to hold your camera up to the eyepiece, so long as you can make it consistent each time you place the camera, you can use piximeter to do the actual measurements after you get your stage micrometer.
1) get piximeter installed 2) get stage micrometer 3) get comfortable shooting down the barrel of the scope 4) take pictures of the stage micrometer with each of the lenses 5) use the pictures from 4. to calibrate piximeter 6) prepare slides, take photos using same method as 4 7) use piximeter to tell you how big the object you photo'd is.
i just went through it recently so the process is fresh.
a reticule is not necessary unless you don't have a computer and a camera, in which case, i'm pretty sure it is. they're only thirty bucks, while a camera and a computer could run you a bit more.  a usb cam would no doubt make everything much more accurate, probably more precise, and easier. Its a pain in the ass trying to get a cam phone to line up from my experience.
congratulations hashfinger! may your coverslips never crack
-------------------- bite my basidiocarp
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: Just Got a Scope! My First Sections... [Re: RuralAnomaly]
#19334959 - 12/27/13 11:29 PM (10 years, 1 month ago) |
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Quote:
RuralAnomaly said:
congratulations hashfinger! may your coverslips never crack

I have only used my phone. It is a pain. ugh. 
Alan is always saying one can use blood cells instead of a micrometer. They are on average 8 nanometers. I think.
Maynard, you could probably get a micrometer at the library as OSU. Or even borrow one from a lab for a bit.
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Just Got a Scope! My First Sections... [Re: RuralAnomaly]
#19335038 - 12/27/13 11:59 PM (10 years, 1 month ago) |
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Quote:
RuralAnomaly said: no, if you make a 'jig' to hold your camera up to the eyepiece, so long as you can make it consistent each time you place the camera, you can use piximeter to do the actual measurements after you get your stage micrometer.
1) get piximeter installed 2) get stage micrometer 3) get comfortable shooting down the barrel of the scope 4) take pictures of the stage micrometer with each of the lenses 5) use the pictures from 4. to calibrate piximeter 6) prepare slides, take photos using same method as 4 7) use piximeter to tell you how big the object you photo'd is.
i just went through it recently so the process is fresh.
a reticule is not necessary unless you don't have a computer and a camera, in which case, i'm pretty sure it is. they're only thirty bucks, while a camera and a computer could run you a bit more.  a usb cam would no doubt make everything much more accurate, probably more precise, and easier. Its a pain in the ass trying to get a cam phone to line up from my experience.
congratulations hashfinger! may your coverslips never crack
 Maynard has a usb cam, do you (hashfinger) need one?
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Byrain

Registered: 01/07/10
Posts: 9,664
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Quote:
Alan Rockefeller said: As long as the camera lens is always the same distance from the eyepiece, I think you should measure digitally.
So no zoom? Some of my better micro images were with zoom...
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 8 months
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Re: Just Got a Scope! My First Sections... [Re: Ganzig]
#19336097 - 12/28/13 08:56 AM (10 years, 1 month ago) |
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Quote:
Ganzig said:
Quote:
RuralAnomaly said:
congratulations hashfinger! may your coverslips never crack

I have only used my phone. It is a pain. ugh. 
Alan is always saying one can use blood cells instead of a micrometer. They are on average 8 nanometers. I think.
Maynard, you could probably get a micrometer at the library as OSU. Or even borrow one from a lab for a bit.
Ha! That's a good one RA... Blood cells? Is Alan really that hardcore? "Hold on lemme measure these P. yungensis spores... Okay now just drip some of that blood on'ere..."
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 8 months
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Re: Just Got a Scope! My First Sections... [Re: Joust]
#19336106 - 12/28/13 08:59 AM (10 years, 1 month ago) |
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Quote:
Joust said:
Quote:
RuralAnomaly said: no, if you make a 'jig' to hold your camera up to the eyepiece, so long as you can make it consistent each time you place the camera, you can use piximeter to do the actual measurements after you get your stage micrometer.
1) get piximeter installed 2) get stage micrometer 3) get comfortable shooting down the barrel of the scope 4) take pictures of the stage micrometer with each of the lenses 5) use the pictures from 4. to calibrate piximeter 6) prepare slides, take photos using same method as 4 7) use piximeter to tell you how big the object you photo'd is.
i just went through it recently so the process is fresh.
a reticule is not necessary unless you don't have a computer and a camera, in which case, i'm pretty sure it is. they're only thirty bucks, while a camera and a computer could run you a bit more.  a usb cam would no doubt make everything much more accurate, probably more precise, and easier. Its a pain in the ass trying to get a cam phone to line up from my experience.
congratulations hashfinger! may your coverslips never crack
 Maynard has a usb cam, do you (hashfinger) need one?
I have a Nikon digital camera. I think if I do like someone said and make a fixture/mounting device so the camera is always the same distance, I'll get away with murder. First things first I need to order a stage micrometer and download some software. You tell me... Do I need a usb cam?
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19336265 - 12/28/13 09:48 AM (10 years, 1 month ago) |
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Quote:
Hashfinger said:
Quote:
Joust said:
Quote:
RuralAnomaly said: no, if you make a 'jig' to hold your camera up to the eyepiece, so long as you can make it consistent each time you place the camera, you can use piximeter to do the actual measurements after you get your stage micrometer.
1) get piximeter installed 2) get stage micrometer 3) get comfortable shooting down the barrel of the scope 4) take pictures of the stage micrometer with each of the lenses 5) use the pictures from 4. to calibrate piximeter 6) prepare slides, take photos using same method as 4 7) use piximeter to tell you how big the object you photo'd is.
i just went through it recently so the process is fresh.
a reticule is not necessary unless you don't have a computer and a camera, in which case, i'm pretty sure it is. they're only thirty bucks, while a camera and a computer could run you a bit more.  a usb cam would no doubt make everything much more accurate, probably more precise, and easier. Its a pain in the ass trying to get a cam phone to line up from my experience.
congratulations hashfinger! may your coverslips never crack
 Maynard has a usb cam, do you (hashfinger) need one?
I have a Nikon digital camera. I think if I do like someone said and make a fixture/mounting device so the camera is always the same distance, I'll get away with murder. First things first I need to order a stage micrometer and download some software. You tell me... Do I need a usb cam?
nope not if you are using a cmaera, get a microscope adapter for your body if you can.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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