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OfflineLyghtsout
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Registered: 10/18/13
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Is it really necessary to put foil back on substrate after inoculation?
    #19335013 - 12/27/13 11:47 PM (10 years, 1 month ago)

The way I see it, the vermiculite would prevent any bacteria from getting in and putting foil on it after inoculation is stupid. I could be wrong tho. The way I'm doing it is after inoculation not covering it with foil or tape just placing it in a sterlite tub and closing it. That way I'd get maximum gas exchange and it would be better protected from dust and bacteria coming from the room.

I am a noob tho so only time will tell if this is the way to do it lol, input from experienced growers is always needed


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Offlineevileye001
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: Lyghtsout]
    #19335019 - 12/27/13 11:51 PM (10 years, 1 month ago)

dont really need much gas exchange during inoculation, just fruiting. just adds extra layer of protection. not saying it wont work without it just saying its a tried and true method for a reason.


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Offlineinvitro


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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: Lyghtsout]
    #19335023 - 12/27/13 11:53 PM (10 years, 1 month ago)

Your right about not keeping the foil on, keeping the foil on would restrict GE and slow down the growth, I know there are many growers who will back me up on this.  You could also wrap the lid up in a coffee filter with a rubber band.
You might do well to go to www.mushroomvideos.com if you want to learn even better ways of doing things.


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Offlinenksfo5
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: evileye001]
    #19335026 - 12/27/13 11:55 PM (10 years, 1 month ago)

Leaving it off you should be fine I've successfully colonized brf cakes with and without the tinfoil bring aced back on after inoculation the reason for the tin foil is to keep water from the pressure cooker gitting inside of the dry Vern layer which acts as a protective layer against contaminations


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Offlinenksfo5
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: nksfo5]
    #19335027 - 12/27/13 11:56 PM (10 years, 1 month ago)

There's no real primal reason for the tin foil other than that


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OfflineLyghtsout
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: nksfo5]
    #19335049 - 12/28/13 12:02 AM (10 years, 1 month ago)

That's what I was thinking, you'd only need the syringe to be sterile and still clean air because after the spores are past vermiculite layer it's pretty well protected. I'm also using a closed sterlite tub as added protection, I see foil directly over the holes serving no purpose other then restricting GE which would slow colonization.


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Invisiblejboredone
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: Lyghtsout]
    #19335067 - 12/28/13 12:05 AM (10 years, 1 month ago)

not foil....:tinfoil:


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InvisiblebodhisattaMDiscordReddit
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: jboredone]
    #19335085 - 12/28/13 12:10 AM (10 years, 1 month ago)

Everything in every BRF tek worth it's salt I have ever read already says to take the foil off after sterilization anyway when using a dry verm barrier. :shrug:


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OfflinePussyFart
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: Lyghtsout]
    #19335441 - 12/28/13 03:13 AM (10 years, 1 month ago)

Quote:

Lyghtsout said:
Is it really necessary to put foil back on substrate after inoculation?



No......you are supposed to remove the foil after sterilization and keep it off.

It is only there to stop water from dripping on your lids...


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OfflinePussyFart
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: invitro]
    #19335442 - 12/28/13 03:14 AM (10 years, 1 month ago)

Quote:

invitro said:
Your right about not keeping the foil on, keeping the foil on would restrict GE and slow down the growth



No, it wouldn't.............the foil is not sealing the jar air tight.


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Offlinebluecap
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: PussyFart]
    #19335901 - 12/28/13 07:45 AM (10 years, 1 month ago)

take the foil off for sure. if left on, it just pools evaporated water onto your filter, or polyfill. and can easily wick bacteria into your jar.:thumbdown: :mushroom2:


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Offlineinvitro


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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: PussyFart]
    #19336068 - 12/28/13 08:50 AM (10 years, 1 month ago)

I agree on the condensation bit, and it doesn't have to be air tight to restrict flow.

If you recall, tall 1/2 pint jars for example can stall before full colonization and sometimes the remedy is to turn them upside down to help the air flow through the cake.  Thus pointing to the importance of not restricting air flow.

Another example, being a wide might quart lid the RR way, with 1/8 or 1/16th inch holes in 3-4 spots, instead of using individual sfd patches you are supposed to use the whole disk in the scenario because not doing so would restrict the way air flows through the holes, so I'm told.  It seems to me that foil would interfere.


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OfflinePussyFart
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: invitro]
    #19336119 - 12/28/13 09:03 AM (10 years, 1 month ago)

Quote:

invitro said:
I agree on the condensation bit, and it doesn't have to be air tight to restrict flow.



Well air passes through the path of least resistance, so if there is an opening of any kind it will flow.

Quote:

invitro said:
If you recall, tall 1/2 pint jars for example can stall before full colonization and sometimes the remedy is to turn them upside down to help the air flow through the cake.  Thus pointing to the importance of not restricting air flow.



The tall jars stall because the substrate gets compacted from gravity, not from lack of GE, hence why the short half pints are recommended.

The hindrance of GE in the tall jars vs short jars is negligible.


Edited by PussyFart (12/28/13 09:05 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Is it really necessary to put foil back on substrate after inoculation? [Re: PussyFart]
    #19336448 - 12/28/13 10:43 AM (10 years, 1 month ago)

Latex balloons will significantly deflate over 24 hours. Gas builds up the resulting little bit of pressure ill make it find its way out through foil or not, its not as restricted as a latex film and even that has gas exchange.  This is why we cant pasteurize in an oven even with a foil lid sealed up


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