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OfflineKtulu369
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Safeguards Against Contaminations
    #19334072 - 12/27/13 06:46 PM (10 years, 1 month ago)

Hi all -

I hope this isn't too vague of a question, but I thought about asking this since I found out that changing the acidity of the substrate can decrease the likelihood of Trichoderma. Are there any other steps you can take in the beginning (besides being extra careful in sterilizing, obviously) to create a substrate which is harder for certain bacteria to grow on, but still okay for mycelium growth?


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Offlinemonoculture
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Re: Safeguards Against Contaminations [Re: Ktulu369]
    #19334130 - 12/27/13 07:07 PM (10 years, 1 month ago)

Quote:

since I found out that changing the acidity of the substrate can decrease the likelihood of Trichoderma



Do you have a link to that information?


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OfflineKtulu369
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Re: Safeguards Against Contaminations [Re: monoculture]
    #19355029 - 01/01/14 02:57 PM (10 years, 29 days ago)

Quote:

monoculture said:
Quote:

since I found out that changing the acidity of the substrate can decrease the likelihood of Trichoderma



Do you have a link to that information?




Not specifically, no. Since starting this thread (and before) I've been trying to relocate where I saw that. I'm starting to think now that I misread something and came away with some misinformation.


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OfflineMAIA
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Re: Safeguards Against Contaminations [Re: Ktulu369]
    #19358471 - 01/02/14 01:13 PM (10 years, 28 days ago)

I don't know if has already been tested by someone over here at the shroomery but used coffee grounds are used to grow mushrooms. It has a higher, but safe, acidity level and it really helps to keep contams away.

I haven't grown for some time but I am myself very curious and I'll give it a try when I have some more time. Meanwhile, please test it yourself. I would use 1/2 brf and 1/2 used coffee grounds in the ratio of the nutritive element. You can also try using just coffee grounds or even use different ratios and understand what works better.

Some interesting stuff:

http://faq.gardenweb.com/faq/lists/soil/2002015354019975.html





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Edited by MAIA (01/02/14 01:16 PM)


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OfflineKtulu369
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Re: Safeguards Against Contaminations [Re: MAIA]
    #19362506 - 01/03/14 09:05 AM (10 years, 28 days ago)

Quote:

MAIA said:
I don't know if has already been tested by someone over here at the shroomery but used coffee grounds are used to grow mushrooms. It has a higher, but safe, acidity level and it really helps to keep contams away.




I've just started dabbling in using coffee grounds in substrate, but not quite to the extent of 1:1 because I think I recall RR saying coffee grounds are in fact more prone to contamination than substrate without it, but it can really help the colonization. I'll have to do some more research on that, and see if increasing the ratio helps at all.


I also dug those videos (haven't gotten around to reading the article yet :wink:) but I'm curious about something I heard in there. It said that the substrate is a mixture of coffee grounds and espresso grounds, with mushroom root mixed in. Are they actually just talking about the stem of the mushroom? Their process seemed pretty simple, yet bountiful. :blush: Thanks for sharing!


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OfflineMAIA
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Re: Safeguards Against Contaminations [Re: Ktulu369]
    #19363164 - 01/03/14 12:19 PM (10 years, 27 days ago)

Quote:

Ktulu369 said:
It said that the substrate is a mixture of coffee grounds and espresso grounds, with mushroom root mixed in. Are they actually just talking about the stem of the mushroom? Their process seemed pretty simple, yet bountiful.




The "mushroom root" they refer to is colonized grain. They are using it as spawn. I believe a more accessible wording is used to explain the process.


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InvisibleGanzig
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Re: Safeguards Against Contaminations [Re: MAIA]
    #19363703 - 01/03/14 02:39 PM (10 years, 27 days ago)

Your question can be answered in multiple ways depending on what species you
are attempting to grow.

Avoid coffee grounds. They are a nightmare and have a low success rate. I know from
experience and many will say the same thing.

What are you growing?


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OfflineCrumist
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Re: Safeguards Against Contaminations [Re: Ktulu369]
    #19363791 - 01/03/14 03:02 PM (10 years, 27 days ago)

On the other hand, I believe that having a slightly acidic environment enables the growth of a broad spectrum of other bacteria (fear Clostridium botulinum and C. tetani!). The high pH might be preferable, just a point for consideration.

Or you could crank the pH down real low to below 4.5 and avoid most of the nasty soil bacteria. I wonder how well cubes do in that situation though.


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OfflineKtulu369
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Re: Safeguards Against Contaminations [Re: Crumist]
    #19363875 - 01/03/14 03:19 PM (10 years, 27 days ago)

Quote:

Ganzig said:
Your question can be answered in multiple ways depending on what species you
are attempting to grow.

What are you growing?




P. Cubensis.

Quote:

Crumist said:
On the other hand, I believe that having a slightly acidic environment enables the growth of a broad spectrum of other bacteria (fear Clostridium botulinum and C. tetani!). The high pH might be preferable, just a point for consideration.




Is there a general pH that is considered the "standard" for growing mushrooms, and is there any reason the optimal pH would be different being substrate and casing? I have a printout which states "The preferred pH for a casing is 6.5 to 8 - the optimal pH is 7.5."


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InvisibleGanzig
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Re: Safeguards Against Contaminations [Re: Ktulu369]
    #19363889 - 01/03/14 03:22 PM (10 years, 27 days ago)

If you are using jars in a pf tek then no. There is nothing to do other than
follow sterile techniques.

If you are spawning to straw or poo and straw you can pasteurize the substrate.

This is optimal and that will kill bad bacteria.


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I must keep reminding myself of this. I must keep reminding myself of this.
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OfflineKizzle
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Re: Safeguards Against Contaminations [Re: Ganzig]
    #19364695 - 01/03/14 05:46 PM (10 years, 27 days ago)

Are you talking about spawn or bulk material? Both are considered substrates. Ph seems to be most important when working with casing layers. Most fungi grow better on slightly acidic substrate, but it's more favorable to molds than to the mushroom mycelium.


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