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OfflineTheMustardTiger
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Registered: 08/19/13
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Last seen: 6 years, 6 months
Myc or Mold?
    #19333823 - 12/27/13 05:45 PM (10 years, 1 month ago)



These are my first grain jars and I have a few concerns that a more seasoned veteran might be able to help me with. I inoculated these 2 jars with P. Cubensis, Ecuador and after about 6 days I noticed signs of germination. My concern is that the supposed mycelium seems to be much fuzzier than the myc in my previous BRF jars. To my knowledge, Cubensis mycelium should be exclusively rhizomorphic. Here are some pictures; They aren't the best but I think they should help a little. Could this be some other sort of white mold or am I just overreacting? Thanks in advanced, everyone.

Cory


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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 2 months, 14 days
Re: Myc or Mold? [Re: TheMustardTiger]
    #19333843 - 12/27/13 05:48 PM (10 years, 1 month ago)

In my opinion, the one on the left looks good, the one on the right looks like you didn't hydrate the grains enough and the myc is stressed.  I could easily be wrong though about the second jar.


Edited by invitro (12/27/13 05:50 PM)


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19333862 - 12/27/13 05:52 PM (10 years, 1 month ago)

Quote:

invitro said:
the one on the right looks like you didn't hydrate the grains enough and the myc is stressed.  I could easily be wrong though about the second jar.




These 2 grain jars were from the same batch of grains that I soaked so this is highly improbable. The one on the right has been shaken twice and recently. This may explain the stressed look.


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333870 - 12/27/13 05:54 PM (10 years, 1 month ago)

Ok, there is never a reason IMO to shake more than once.  You can shake between 10 and 30% IMO, others will say 20-30%.  Why did you shake twice?


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InvisibleWilly Wonka
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Re: Myc or Mold? [Re: invitro]
    #19333882 - 12/27/13 05:56 PM (10 years, 1 month ago)

They both look good to me:thumbup:


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AMU



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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19333889 - 12/27/13 05:58 PM (10 years, 1 month ago)

Well I was concerned about whether or not it was mold so I shook it the second time to see how it grew back. I just remember my BRF jars being extra ropey and this stuff just looks like fuzz. I've seen other grain jars with cubensis myc that look much ropier and thick. The stuff in these jars just seemed too fuzzy for comfort.


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333901 - 12/27/13 06:01 PM (10 years, 1 month ago)

That is a good reason to shake a second time.  Different genetic will give you different looks, super-fluffy may not be a good producer.  I would not use that for a master jar if I were you.


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19333915 - 12/27/13 06:07 PM (10 years, 1 month ago)

I may just fruit these 2 jars separately with a little bit of coir/verm and see what happens. I just want some decent tissue to clone so I can get some quality product. A generous member did send me a Penis Envy print with which I made about 11 syringes. I' going to knock up 5 or so grain jars next week with that strain and see how it fares. I'm working without a flow hood at the moment so agar isn't exactly practical at this point.


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Offlinemagickspore
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333928 - 12/27/13 06:10 PM (10 years, 1 month ago)

You can do agar in a SAB just fine.


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Offlineinvitro


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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333929 - 12/27/13 06:10 PM (10 years, 1 month ago)

How about reading a no-pour agar tek?  No flow hood needed let's see if I can find a good one, I'm trying to learn that myself.


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19333940 - 12/27/13 06:12 PM (10 years, 1 month ago)

I guess I'm just under the impression that the flow hood is god. Plus I've been having bad luck with this stuff lately and I guess I've attributed my failures to the lack of equipment and sterile environment. The last 2 times I knocked up brf jars I got zero germination with 12 jars.


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333958 - 12/27/13 06:18 PM (10 years, 1 month ago)

Lack of good equipment would only lead to contams, you should have had some kind of growth, which might point towards something wrong with your syringe (spores no longer viable).  How long did you wait to see growth in the brf jars before calling it quits?


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19333974 - 12/27/13 06:22 PM (10 years, 1 month ago)

The last jars I let set for about 2 1/2 weeks before I tossed them. My very first set of jars all colonized just fine, but didn't fruit very well. I narrowed that down to bad spores from a not so reputable vendor. The last 2 sets of brf jars were inoculated with spores from a trusted sponsor of this website. My only guess could be that something went wrong during the sterilization process. Oddly enough, I have about 9 newer brf jars that I knocked up about a week ago and I've got nothing on those either. Very bad luck with MS.


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19333980 - 12/27/13 06:24 PM (10 years, 1 month ago)

It can take time sometimes, what's your room temperature?  I would give it 4 weeks before quitting.


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19334025 - 12/27/13 06:35 PM (10 years, 1 month ago)

Room always stays above 70F at the very least. Ambient temperature is usually 73. 4 weeks seems like a long time. wouldn't the sub start drying out by then?


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19334041 - 12/27/13 06:39 PM (10 years, 1 month ago)

Yes it would, that's one reason why experienced people use agar wedges or liquid culture.  Have you seen www.mushroomvideos.com ? $9 to download priceless informative videos.


Edited by invitro (12/27/13 06:41 PM)


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19334052 - 12/27/13 06:41 PM (10 years, 1 month ago)

I have all of the stuff for agar, maybe i'll give it a whirl in the SAB with some of the Penis envy MS.


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Offlineinvitro


Registered: 05/03/13
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19334057 - 12/27/13 06:43 PM (10 years, 1 month ago)

You might read this first:

a no pour agar tek

http://www.shroomery.org/forums/showflat.php/Number/14788118

Also PE is said to be slow in general by many cultivators.


Edited by invitro (12/27/13 06:44 PM)


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: invitro]
    #19334074 - 12/27/13 06:47 PM (10 years, 1 month ago)

Very nice. I'll definitely consider this.


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OfflineTheMustardTiger
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Re: Myc or Mold? [Re: TheMustardTiger]
    #19338027 - 12/28/13 05:56 PM (10 years, 1 month ago)

Here are some updated pictures of the 2 jars



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