Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineQuexl
Male
Registered: 12/17/13
Posts: 1,443
Loc: Flag
Last seen: 8 years, 10 months
Curing jars and colonization time.
    #19333024 - 12/27/13 01:28 PM (10 years, 1 month ago)

Two jars from a batch were forgotten about 2 months ago, then inoculated with the left overs from the same syringe as the original batch. They were always left sealed in an incubator @ 80f. The syringe was stored at 70f in a drawer. The mycelium in two cured jars is developing far faster than the other jars:

Not 'cured' for 30d(n:12)
Mean % cover @ 30d: 56.00%

cured for 30d(2)
Mean % covered @ 15d(*2): 70%

~14% increase?

Has anyone noticed that brf cakes that are left to 'cure' do better than other ones?


--------------------
grain spawn bacteria info


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 1 hour, 43 minutes
Trusted Cultivator
Re: Curing jars and colonization time. [Re: Quexl]
    #19333045 - 12/27/13 01:35 PM (10 years, 1 month ago)

Cure :wtf:

Doubt it has anything to do with the jar "curing"
Probably just a faster growing culture


Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Curing jars and colonization time. [Re: Stromrider]
    #19333188 - 12/27/13 02:24 PM (10 years, 1 month ago)

weird.  only thing i could think of is the jars were initially too wet and these that were left for 2 months dried out a bit reaching perfect moisture content.  or its not cube myc and ur seeing white growth of a mold  or contam.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


Extras: Filter Print Post Top
OfflineQuexl
Male
Registered: 12/17/13
Posts: 1,443
Loc: Flag
Last seen: 8 years, 10 months
Re: Curing jars and colonization time. [Re: blindingleaf]
    #19333968 - 12/27/13 06:20 PM (10 years, 1 month ago)

The ingredients for all jars were mixed individually in a beaker to precise measurements and washed out between containers (g. cylinder measured all, de-ionized water, mixed dry and wetted in 3 poured additions of water). The growth is rhizomorphic and not a fast-growing contaminant.. But that would be my first guess too.

I agree it's most likely a faster growing culture, but I'm going to stagger my inoculation 3 days per jar in the next batch and see if there's a correlation ("curing time" vs. colonization time). The CFU on the jars that were left 30d was way higher than the original jars also, for example, 6-7 CFU opposed to 1-4 CFU on the regular batch.

Thanks everyone for your input, my results will appear in this thread. :smile:

(CFU = colony forming units; ie: successful inoculation points.)


--------------------
grain spawn bacteria info


Extras: Filter Print Post Top
Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: Curing jars and colonization time. [Re: Quexl]
    #19334228 - 12/27/13 07:44 PM (10 years, 1 month ago)

30 days to 56% colonization sounds really slow.  maybe the internal temps are too high in the jar?  Somethings not right IMO.


Extras: Filter Print Post Top
OfflineQuexl
Male
Registered: 12/17/13
Posts: 1,443
Loc: Flag
Last seen: 8 years, 10 months
Re: Curing jars and colonization time. [Re: invitro]
    #19334335 - 12/27/13 08:14 PM (10 years, 1 month ago)

It is slow! :smile: I also think maybe the spores were not hydrated at inoculation.

But remember 56% is the mean(u). And in all honesty a totally high standard deviation, ie some where at 40% some where at 90% of n=12.

I'll correlate it eventually, thanks for the reply. :smile:


--------------------
grain spawn bacteria info


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Quart jar colonization - when complete? farmer_maggot 5,634 1 09/12/01 02:48 AM
by NDK
* Help: top of jar colonizing sloow FungusFarmer 1,863 3 09/06/01 10:15 AM
by puscle
* 1/30 jars colonized in 12 days :o( shroomatomic 1,881 3 03/12/16 02:14 PM
by Leftfield420
* Jar colonize question? Anonymous 1,871 2 03/25/02 03:54 PM
by Anonymous
* Time for jar colonization AbeZard 1,679 5 07/25/01 07:14 PM
by AbeZard
* Ever have your jars colonize from bottom to top? straightbangin 1,653 2 01/10/02 12:44 PM
by MAIA
* 2 jars colonized without PC... Hell Yeah Raven_420 1,112 2 04/13/02 02:47 PM
by Raven_420
* jars colonizing MycoGlowFlow 976 4 09/07/01 05:33 PM
by MycoGlowFlow

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
345 topic views. 36 members, 230 guests and 27 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.009 seconds on 14 queries.