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Quexl

Registered: 12/17/13
Posts: 1,443
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Last seen: 8 years, 10 months
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Curing jars and colonization time.
#19333024 - 12/27/13 01:28 PM (10 years, 1 month ago) |
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Two jars from a batch were forgotten about 2 months ago, then inoculated with the left overs from the same syringe as the original batch. They were always left sealed in an incubator @ 80f. The syringe was stored at 70f in a drawer. The mycelium in two cured jars is developing far faster than the other jars:
Not 'cured' for 30d(n:12) Mean % cover @ 30d: 56.00%
cured for 30d(2) Mean % covered @ 15d(*2): 70%
~14% increase?
Has anyone noticed that brf cakes that are left to 'cure' do better than other ones?
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 1 hour, 43 minutes
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Re: Curing jars and colonization time. [Re: Quexl]
#19333045 - 12/27/13 01:35 PM (10 years, 1 month ago) |
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Cure 
Doubt it has anything to do with the jar "curing" Probably just a faster growing culture
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Curing jars and colonization time. [Re: Stromrider]
#19333188 - 12/27/13 02:24 PM (10 years, 1 month ago) |
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weird. only thing i could think of is the jars were initially too wet and these that were left for 2 months dried out a bit reaching perfect moisture content. or its not cube myc and ur seeing white growth of a mold or contam.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Quexl

Registered: 12/17/13
Posts: 1,443
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Last seen: 8 years, 10 months
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Re: Curing jars and colonization time. [Re: blindingleaf]
#19333968 - 12/27/13 06:20 PM (10 years, 1 month ago) |
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The ingredients for all jars were mixed individually in a beaker to precise measurements and washed out between containers (g. cylinder measured all, de-ionized water, mixed dry and wetted in 3 poured additions of water). The growth is rhizomorphic and not a fast-growing contaminant.. But that would be my first guess too.
I agree it's most likely a faster growing culture, but I'm going to stagger my inoculation 3 days per jar in the next batch and see if there's a correlation ("curing time" vs. colonization time). The CFU on the jars that were left 30d was way higher than the original jars also, for example, 6-7 CFU opposed to 1-4 CFU on the regular batch.
Thanks everyone for your input, my results will appear in this thread. 
(CFU = colony forming units; ie: successful inoculation points.)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Curing jars and colonization time. [Re: Quexl]
#19334228 - 12/27/13 07:44 PM (10 years, 1 month ago) |
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30 days to 56% colonization sounds really slow. maybe the internal temps are too high in the jar? Somethings not right IMO.
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Quexl

Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 10 months
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Re: Curing jars and colonization time. [Re: invitro]
#19334335 - 12/27/13 08:14 PM (10 years, 1 month ago) |
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It is slow! I also think maybe the spores were not hydrated at inoculation.
But remember 56% is the mean(u). And in all honesty a totally high standard deviation, ie some where at 40% some where at 90% of n=12.
I'll correlate it eventually, thanks for the reply.
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