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Invisibleelasticaltiger
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This is why you PC your jars immediately - UPDATED - SUCCESS
    #19331442 - 12/27/13 01:43 AM (10 years, 1 month ago)

I got some new strainers the other day. The result was I lost a lot less grain during the drying time so I had enough left over for an extra master jar that wouldn't fit in my pc.

I PC'd the first 7 but then I had to leave the next day for christmas stuff.  So I just figured I'd PC it when I got back after three days.



I am actually holding the jar upside down to illustrate how 'fully colonized' the mold is.  Not a single loose grain.

This is 96 hours of mold growth on rye.  If that's 96 hours I would imagine that even waiting as little as 16 hours would have too much germinated mold growth.

Un sterilized grain jars are only good for a matter of hours I guess.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

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Edited by elasticaltiger (03/15/14 11:06 PM)


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Offlineforrest
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19331456 - 12/27/13 01:51 AM (10 years, 1 month ago)

that's really fast! at what temps did it colonise?

i would think, as long as the mold didn't sporulate, it would be good to pc. mold/germinated spores are easyer to kill with heat than spores, so i can imagine it might even be a plus to first let them germinate (like already happens to some extend when you soak the grain for 12-24 hours).


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: forrest]
    #19331468 - 12/27/13 01:57 AM (10 years, 1 month ago)

They colonized between 75 and 80 degrees. I left the heat on while I was gone cause I are smart and I hate money. :derp:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineOgreLokon
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19331480 - 12/27/13 02:04 AM (10 years, 1 month ago)

PLEASE tell me that wasn't the Zero strain!

:privatejoke:


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OfflinecronicrFacebook
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Re: This is why you PC your jars immediately [Re: OgreLokon]
    #19331487 - 12/27/13 02:10 AM (10 years, 1 month ago)

Quote:

OgreLokon said:
PLEASE tell me that wasn't the Zero strain!

:privatejoke:



no....it was mold


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: OgreLokon]
    #19331505 - 12/27/13 02:26 AM (10 years, 1 month ago)

Quote:

OgreLokon said:
PLEASE tell me that wasn't the Zero strain!

:privatejoke:




:laugh2:  Fortunately no. Nothing inoculated this jar. This was just grain that got soaked and then boiled then dried but never got inoculated.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflinecronicrFacebook
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Re: This is why you PC your jars immediately [Re: elasticaltiger] * 1
    #19331510 - 12/27/13 02:28 AM (10 years, 1 month ago)

crazy how quick that shit is hey, are you gonna g2g that shit?


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: cronicr]
    #19331519 - 12/27/13 02:34 AM (10 years, 1 month ago)

I guess I should, it's a super fast strain and I wouldn't want to lose genetics like that!


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflinecronicrFacebook
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19331522 - 12/27/13 02:35 AM (10 years, 1 month ago)

contam timelapse would be cool


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OfflineStromriderM
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Re: This is why you PC your jars immediately [Re: cronicr]
    #19331544 - 12/27/13 02:53 AM (10 years, 1 month ago)

:lol: once again you suck tiger :tongue:


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OfflineCMOS
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Re: This is why you PC your jars immediately [Re: Stromrider]
    #19331682 - 12/27/13 03:57 AM (10 years, 1 month ago)

How long did you soak the grains before this?


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Offlineshroomer
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Re: This is why you PC your jars immediately [Re: CMOS]
    #19331705 - 12/27/13 04:14 AM (10 years, 1 month ago)

You should PC it again and inoculate and see what happens


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: CMOS]
    #19331961 - 12/27/13 07:17 AM (10 years, 1 month ago)

Quote:

CMOS said:
How long did you soak the grains before this?




Standard overnight soak.  Probably 20 hours.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflinePaulsage
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19332292 - 12/27/13 09:35 AM (10 years, 1 month ago)

it's be interesting to PC it and then noc it up or do a g2g just to see how the jar turns out...  no expectations from it other than experimentation.


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

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OfflineGod Stamet
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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19332402 - 12/27/13 10:13 AM (10 years, 1 month ago)

looks like yeast


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19332834 - 12/27/13 12:35 PM (10 years, 1 month ago)

Quote:

Paulsage said:
it's be interesting to PC it and then noc it up or do a g2g just to see how the jar turns out...  no expectations from it other than experimentation.




That is an AWESOME idea. I will do this. Stay tuned.

EDIT: But there is no fucking way I'm opening this jar. It's going to be multispore injection.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (12/27/13 12:36 PM)


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OfflineStromriderM
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19332916 - 12/27/13 01:01 PM (10 years, 1 month ago)

I look forward to seeing this

I'm pretty sure that's mostly yeast growing in there


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Re: This is why you PC your jars immediately [Re: Stromrider]
    #19332947 - 12/27/13 01:08 PM (10 years, 1 month ago)

as long as you pc it opening it shouldn't be a problem.....i mean the worst that could happen is it contams lol


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Invisibled0urd3n
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Re: This is why you PC your jars immediately [Re: cronicr]
    #19333015 - 12/27/13 01:26 PM (10 years, 1 month ago)

I am very curious what happens. Make sure to update :thumbup:


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Offlinefractaldill
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19333152 - 12/27/13 02:11 PM (10 years, 1 month ago)

i was expecting to see a bunch of rye sprouting.


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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19333215 - 12/27/13 02:35 PM (10 years, 1 month ago)

Quote:

elasticaltiger said:
Quote:

Paulsage said:
it's be interesting to PC it and then noc it up or do a g2g just to see how the jar turns out...  no expectations from it other than experimentation.




That is an AWESOME idea. I will do this. Stay tuned.

EDIT: But there is no fucking way I'm opening this jar. It's going to be multispore injection.




dooooooo eeeeeeeet...

but yah, if you PC the jar it should kill anything in it and that'd be safe to G2G...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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OfflineSynKyd
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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19333227 - 12/27/13 02:37 PM (10 years, 1 month ago)

:popcorn:


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: SynKyd]
    #19339085 - 12/28/13 09:36 PM (10 years, 1 month ago)

The jar is PCing right now. Gonna give it a full two hours.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineCMOS
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19339820 - 12/29/13 12:57 AM (10 years, 1 month ago)

I think you will probably end up with a mushy cake after PCing it, but you should be able to colonize it.  :shrug:  Good luck getting it out of the jar. :thumbup:

Let us know how it goes after PCing.


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Offlinemagickspore
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Re: This is why you PC your jars immediately [Re: CMOS]
    #19339938 - 12/29/13 01:38 AM (10 years, 1 month ago)

Well was it a lifeless mass of mushy mycelium or what?


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: magickspore]
    #19340064 - 12/29/13 02:43 AM (10 years, 1 month ago)

So the PCing is done.  Here is the jar before it was shaken:

 


It was definitely all stuck together like a cake.  So I shook it up.

 


After the shake it actually looks pretty normal with the exception of some dead 'yeast' bits stuck to the glass.  I will inoculate tomorrow.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineCMOS
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19340094 - 12/29/13 03:02 AM (10 years, 1 month ago)

Last pic actually doesn't look that bad...


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OfflinePaulsage
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19384768 - 01/07/14 08:02 PM (10 years, 23 days ago)

Quote:

After the shake it actually looks pretty normal with the exception of some dead 'yeast' bits stuck to the glass.  I will inoculate tomorrow.




so...?


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19384972 - 01/07/14 08:45 PM (10 years, 23 days ago)

NIce, curious to see if that survived.

I've put extra grain jars in the fridge for a few days before pc'ing them and they've been fine... room temps are bad mmmkay.


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: MisterCoOo]
    #19385128 - 01/07/14 09:14 PM (10 years, 23 days ago)

I inoculated with a multispore syringe, Ecuador, 1 cc on the 30th and I have yet to see any growth. Not a good sign but I've been surprised before.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinebowserk
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Re: This is why you PC your jars immediately [Re: fractaldill]
    #19385169 - 01/07/14 09:23 PM (10 years, 23 days ago)

Quote:

fractaldill said:
i was expecting to see a bunch of rye sprouting.




Same here I soaked some grain overnight. stranded them over night, the next morning i had some sprouts.


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Re: This is why you PC your jars immediately [Re: bowserk]
    #19385201 - 01/07/14 09:28 PM (10 years, 23 days ago)

Quote:

bowserk said:
Quote:

fractaldill said:
i was expecting to see a bunch of rye sprouting.




Same here I soaked some grain overnight. stranded them over night, the next morning i had some sprouts.



simmer and start with scolding water:thumbup:


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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19385206 - 01/07/14 09:29 PM (10 years, 23 days ago)

Quote:

elasticaltiger said:
I inoculated with a multispore syringe, Ecuador, 1 cc on the 30th and I have yet to see any growth. Not a good sign but I've been surprised before.




it's only been 8 days, be patient...  i once had a jar that took 2 weeks just to start showing...


--------------------
"The brain is a reducing valve that restricts consciousness" - A Huxley

"Do not go where the path may lead, go instead where there is no path and leave a trail" – Ralph Waldo Emerson…

"Whatever you study you also change" - Heisenberg Uncertainty principle


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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19385213 - 01/07/14 09:30 PM (10 years, 23 days ago)

i don't think he was complaining, just answering lol


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: Paulsage]
    #19385222 - 01/07/14 09:32 PM (10 years, 23 days ago)

Quote:

Paulsage said:
Quote:

elasticaltiger said:
I inoculated with a multispore syringe, Ecuador, 1 cc on the 30th and I have yet to see any growth. Not a good sign but I've been surprised before.




it's only been 8 days, be patient...  i once had a jar that took 2 weeks just to start showing...




Rofl. Thank you. I will be more patient :wink:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19702038 - 03/15/14 11:05 PM (9 years, 10 months ago)

Well Well Well...

Look who finally decided to germinate...



--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisibleelasticaltiger
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Re: This is why you PC your jars immediately [Re: elasticaltiger]
    #19702186 - 03/15/14 11:56 PM (9 years, 10 months ago)

In case you can't tell from the picture the mycelium is incredibly WHITE / THICK. Any ideas for why this is?  I have other jars that also recently germinated but they're much more straggly  / whispy.


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Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

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