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Us.Two
The Innovator


Registered: 01/29/12 
Posts: 106
Last seen: 3 years, 4 months
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Popcorn+Coffee+Honey Experiment
#19331184 - 12/26/13 11:43 PM (10 years, 1 month ago) |
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Well, I'm out of just about everything except for popcorn that I could use as a substrate and I wanted to try something new. I'll keep everyone updated as to how everything's goin as new ideas come to me. So without further ado here's what I've done so far:
1. Wash popcorn with warm water and Dr. B's Soap (Tea Tree). Rinse 4-5 times.
2. Put steam basket into pot. Pour popcorn in.
3. Fill 1-2 inches above popcorn with filtered or bottled water.
4. Boil for ~ 45 minutes.
5. Fill till water is ~1-2 inches above popcorn again due to evap.
6. Lift basket up, pour 1 tbsp. of coffee grounds under basket, put it back down.
7. Pour ~1.5 tbsp. honey into the water/popcorn/coffee mixture.
8. Cover, boil for another 1.5 hours.
8-1. At this point I took three of my clean syringes and pulled the boiling "broth" into them to use for later adventures.
9. Remove from heat, lift steam basket and dump popcorn onto paper towels to dry.
9-1. After popcorn was removed from broth I put a coffee filter over 2 pint jars and filtered 300ml of broth per jar into them. **More experimentation will be done with this stuff to see how well it may work as a liquid nutrient or LC, most likely diluted**
That is where I'm at so far and as always, questions/comments/concerns are quite welcome.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Popcorn+Coffee+Honey Experiment [Re: Us.Two]
#19331189 - 12/26/13 11:46 PM (10 years, 1 month ago) |
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Make sure you inoculate those LC's with agar
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Us.Two
The Innovator


Registered: 01/29/12 
Posts: 106
Last seen: 3 years, 4 months
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Re: Popcorn+Coffee+Honey Experiment [Re: Pastywhyte]
#19331317 - 12/27/13 12:35 AM (10 years, 1 month ago) |
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I've tried agar one time and all I grew was damn fields of green. I will have to get more and try it again some time soon. What is the second best thing to inoculate LC with?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Popcorn+Coffee+Honey Experiment [Re: Us.Two]
#19331327 - 12/27/13 12:41 AM (10 years, 1 month ago) |
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Quote:
Us.Two said: I've tried agar one time and all I grew was damn fields of green. I will have to get more and try it again some time soon. What is the second best thing to inoculate LC with?
If you can't get proper sterile tek down with agar, then LC is probably a disaster waiting to happen. You could get lucky inoculating an LC with spores or tissue from a fruit but, your chances for contam are very high. Also because LC can contam at anytime, and you can't see it, LC has precautions that should be taken when using it. You want higher capacity syringes, whatman filters for the lids, proper SHIP would be a good idea. IMO agar is simpler and easier all around 
You could half ass an LC and get lucky, but you could also very easily grow a butload of mold. Just to be safe, I would inoculate my LC with agar, and I would test the LC on agar.
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CMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
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Re: Popcorn+Coffee+Honey Experiment [Re: Pastywhyte]
#19331696 - 12/27/13 04:11 AM (10 years, 1 month ago) |
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Quote:
If you can't get proper sterile tek down with agar, then LC is probably a disaster waiting to happen.
If you have the LC already made and spores to spare, go for it. I would rather go spores to grain then spores to an LC though. Even thats risky though, but at least you could visibly identify some contaminants.
I would go for experimenting once you have a baseline proven procedure down, then start changing variables from there.
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Us.Two
The Innovator


Registered: 01/29/12 
Posts: 106
Last seen: 3 years, 4 months
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Re: Popcorn+Coffee+Honey Experiment [Re: CMOS]
#19351958 - 12/31/13 05:15 PM (10 years, 30 days ago) |
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UPDATE:
This stuff is pretty amazing. I inoculated it at 1/4 strength (100ml of the popcorn-coffee-honey mixture and 300ml water combined) with my blender-tek Chocolate Oyster culture and it just took off.

I would assume that it would work REALLY well with other types of mushrooms also.
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