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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Why does this happen to all my jars? [Re: Aero]
#19328607 - 12/26/13 12:19 PM (10 years, 1 month ago) |
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Quote:
Aero said:
Quote:
36fuckin5 said:
Quote:
Aero said: i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
WTF would this teach you about this particular problem?
that the culture+syringe+environment is clean my friend, and the problem is the grain
think before u start raging
Agar would be the right suggestion, cakes can be steamed so the answer wouldn't help much, be sure to vent your steam for a few minutes before putting your weight on
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Why does this happen to all my jars? [Re: cronicr]
#19328618 - 12/26/13 12:23 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
Aero said:
Quote:
36fuckin5 said:
Quote:
Aero said: i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
WTF would this teach you about this particular problem?
that the culture+syringe+environment is clean my friend, and the problem is the grain
think before u start raging
Agar would be the right suggestion, cakes can be steamed so the answer wouldn't help much, be sure to vent your steam for a few minutes before putting your weight on
he is using agar
-------------------- SPREAD THE SPORES
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bootster


Registered: 02/22/11
Posts: 1,531
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Re: Why does this happen to all my jars? [Re: Aero]
#19328628 - 12/26/13 12:26 PM (10 years, 1 month ago) |
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I started out with monotubs and never have had an interest in cakes. I have had nothing but good luck with monotubs and it is frustrating to have everything go by the wayside.
Sterile techniques are the same as they ever were (without going into detail ).
I am thinking about going with plastic lids and SFD patches siliconed on.
I am lazy and when a jar sits with green in it, it actually stains the SFD green. I don't know if I'm comfortable with reusing them. The plastic idea may not work either.
Thank you guys for taking time to answer my questions and try to help me out.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Why does this happen to all my jars? [Re: Aero]
#19328641 - 12/26/13 12:32 PM (10 years, 1 month ago) |
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Quote:
Aero said: that the culture+syringe+environment is clean my friend, and the problem is the grain
think before u start raging
The problem is obviously either inoculant, technique or bad filters.
If you're venting your PC for a few minutes and running a full 15 PSI for 90 minutes, the grain is fine.
The agar plates look clean, so it's not inoculant, because it happens with vendor syringes and agar wedges. He can apparently get clean plates, so I'd imagine he can probably toss one in a grain jar without contaminating it, since it's the same motions.
Therefore, I'm blaming the filters. Polyfil isn't the best thing in the world to use. It can get jostled and open holes, and it also just isn't the best pore size for filtration without many, many layers, and just how many you need hasn't really been established.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: bootster]
#19328651 - 12/26/13 12:33 PM (10 years, 1 month ago) |
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Quote:
bootster said: I started out with monotubs and never have had an interest in cakes. I have had nothing but good luck with monotubs and it is frustrating to have everything go by the wayside.
Sterile techniques are the same as they ever were (without going into detail ).
I am thinking about going with plastic lids and SFD patches siliconed on.
I am lazy and when a jar sits with green in it, it actually stains the SFD green. I don't know if I'm comfortable with reusing them. The plastic idea may not work either.
Thank you guys for taking time to answer my questions and try to help me out.
Lids with SFD glued on are the way to go, hopefully you can just avoid getting the green in your jars in the first place and you won't have to worry about the mold ruining your filters for future use.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Just don't get those thin-ass cheap (they're not even inexpensive, just cheap) SFDs. Get the super thick ones from Out-Grow. And I wouldn't try cutting them, just use them hole. If you have to use a syringe one day, just throw it in your SAB, pop the lid and squirt some in.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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bootster


Registered: 02/22/11
Posts: 1,531
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Quote:
RiparianZoneJunky said:
Quote:
bootster said: I started out with monotubs and never have had an interest in cakes. I have had nothing but good luck with monotubs and it is frustrating to have everything go by the wayside.
Sterile techniques are the same as they ever were (without going into detail ).
I am thinking about going with plastic lids and SFD patches siliconed on.
I am lazy and when a jar sits with green in it, it actually stains the SFD green. I don't know if I'm comfortable with reusing them. The plastic idea may not work either.
Thank you guys for taking time to answer my questions and try to help me out.
Lids with SFD glued on are the way to go, hopefully you can just avoid getting the green in your jars in the first place and you won't have to worry about the mold ruining your filters for future use.
I'm not laughing but you have made a good point. I think I'll pursue that avenue for failure.
Thanks guys.
Here is a load of jars that I did last night.

Edited by bootster (12/26/13 01:20 PM)
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 23 hours
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Re: Why does this happen to all my jars? [Re: 36fuckin5]
#19328829 - 12/26/13 01:08 PM (10 years, 1 month ago) |
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A culture can be contaminated without showing any signs. That scenario usually happens though as a result of the culture becoming contaminated after it is already at least partially colonized, so it'd still most likely be related to your sterile technique.
I could see it possibly being the filter if you treated your control jars differently than the incoulated jar, like if you didn't shake them but shook the incoulated ones or stored them in a different location.
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bootster


Registered: 02/22/11
Posts: 1,531
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Re: Why does this happen to all my jars? [Re: Kizzle]
#19328864 - 12/26/13 01:23 PM (10 years, 1 month ago) |
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You may have a point Kizzle but I usually transfer up to 7 or 8 times (I'm anal about transfers) before I'm confident that the culture "looks" right.
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: bootster]
#19329129 - 12/26/13 02:27 PM (10 years, 1 month ago) |
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Quote:
bootster said: You may have a point Kizzle but I usually transfer up to 7 or 8 times (I'm anal about transfers) before I'm confident that the culture "looks" right.
When I've had cultures get mold from neglect the contam shows up on the next plate after a transfer, so if you're doing multiple transfers and don't see contams on agar it's definitely not your agar wedges. The fact that you work with agar and aren't getting contams on your plates shows that you have good sterile technique in general and making transfers is hardly different in terms of technique than throwing a wedge in a grain jar. It's gotta be your polyfil. I just cut small discs of SFD and silicone glue them over your holes, you don't even need an injection port if you're going to be exclusively noccing with agar. I use this tek and have zero problems. Good luck
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 1 day, 23 hours
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There's nothing wrong with polyfill in general but if it's getting wet that could be a problem.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Why does this happen to all my jars? [Re: Kizzle]
#19329536 - 12/26/13 04:26 PM (10 years, 1 month ago) |
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yea i too use polyfil with zero contam rate..
-------------------- SPREAD THE SPORES
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jellyfish


Registered: 10/02/05
Posts: 7,457
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Re: Why does this happen to all my jars? [Re: Aero]
#19329556 - 12/26/13 04:31 PM (10 years, 1 month ago) |
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I know it's hard to tell but those grains don't look hydrated to me. I'd make sure your pressure cooker is maintaining the correct temperature. Remember, agar will take a lot less time to sterilize then grain.
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Why does this happen to all my jars? [Re: jellyfish]
#19329574 - 12/26/13 04:35 PM (10 years, 1 month ago) |
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Quote:
jellyfish said: I know it's hard to tell but those grains don't look hydrated to me. I'd make sure your pressure cooker is maintaining the correct temperature. Remember, agar will take a lot less time to sterilize then grain.
That is a good point. I don't think anyone else has offered the PC being an issue here
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Why does this happen to all my jars? [Re: PirateSwazey]
#19329687 - 12/26/13 05:02 PM (10 years, 1 month ago) |
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Quote:
PirateSwazey said:
Quote:
jellyfish said: I know it's hard to tell but those grains don't look hydrated to me. I'd make sure your pressure cooker is maintaining the correct temperature. Remember, agar will take a lot less time to sterilize then grain.
That is a good point. I don't think anyone else has offered the PC being an issue here 
i was actually
Quote:
Aero said: might be a problem in the sterilization process not enough pressure in the PC?
i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
-------------------- SPREAD THE SPORES
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Why does this happen to all my jars? [Re: Aero]
#19329706 - 12/26/13 05:07 PM (10 years, 1 month ago) |
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so did i, but i do believe your right about the grains, they look like they didn't get to soak enough
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Why does this happen to all my jars? [Re: cronicr]
#19329731 - 12/26/13 05:13 PM (10 years, 1 month ago) |
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^ Agreed.
I think OP said he was trying to keep them from soaking too long to prevent bacteria. Could be possible that by not soaking long enough he isn't germinating enough endospores 
Sorry I must have missed all the other input concerning the PC...
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Why does this happen to all my jars? [Re: PirateSwazey]
#19329733 - 12/26/13 05:14 PM (10 years, 1 month ago) |
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i think it would be more along the lines of it being harder for the pressure to penetrate but i could be wrong
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Quote:
PirateSwazey said: ^ Agreed.
I think OP said he was trying to keep them from soaking too long to prevent bacteria. Could be possible that by not soaking long enough he isn't germinating enough endospores 
Sorry I must have missed all the other input concerning the PC...
Quote:
RiparianZoneJunky said:
Quote:
PirateSwazey said: Maybe it's like these guys are saying...
something in your grain 
Have you tried pc'ing some WBS n using that since you've been having problems?
Switch to a different source for grain, give it a good long soak, don't add any supplements at first and give it a good solid 90 minutes at 15 PSI. I think it's your grain even though you claim to leave test jars.
Edited by RiparianZoneJunky (12/26/13 05:24 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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as long as he hydrates right the grain choice won't matter When the grains are hydrated, the hard shell of the endospore is also hydrated and will be killed in the pc weather or not it germinated
oops that was a rr qoute i just forgot to qoute instead i just copied
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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