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bootster


Registered: 02/22/11 
Posts: 1,531
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Why does this happen to all my jars?
#19328290 - 12/26/13 10:40 AM (10 years, 1 month ago) |
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I don't have a clue.
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: bootster]
#19328295 - 12/26/13 10:43 AM (10 years, 1 month ago) |
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Have you left any jars not inoculated to see whether it's a bunk syringe/sterile technique or your grain prep?
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bootster


Registered: 02/22/11 
Posts: 1,531
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I always do that and they remain clean. I have 18 jars of different strains/species and it always shows up at once and soon they will have a powdery look to them. After a shake, the green comes out and forms a green cloud in the jar. Some of these are directly injected by a syringe from a vendor with a red hot needle into an RTV injection port. 
Most come from clean looking agar cultures.
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Mrdbrewer
Mr



Registered: 06/17/13
Posts: 3,974
Loc: Galafrey.
Last seen: 4 years, 1 month
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Re: Why does this happen to all my jars? [Re: bootster]
#19328319 - 12/26/13 10:53 AM (10 years, 1 month ago) |
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maybe change the substrate supplier????
-------------------- Constantly checking my dick and nips to see if im not dying
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Juicin
Stranger

Registered: 10/27/13
Posts: 897
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Re: Why does this happen to all my jars? [Re: bootster]
#19328326 - 12/26/13 10:55 AM (10 years, 1 month ago) |
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If you're using clean wedges i'd try changing spawn supplier...........
If you can do agar work in your house but can't get grains going that's weird as fuck. And i'd start by getting new grains
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Why does this happen to all my jars? [Re: bootster]
#19328327 - 12/26/13 10:55 AM (10 years, 1 month ago) |
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you sure your agar has mushroom myc and not a similar lookin mold myc growing on it?
are you inoculating your dishes with clone tissue or spores?
I usually only catch bacteria on my petris but I have inoculated a jar with what looked like a wedge of mushroom myc and turned out to be the green. it grew with a ghost or grey looking myc on the grain though... not what yours looks like.
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bootster


Registered: 02/22/11 
Posts: 1,531
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Re: Why does this happen to all my jars? [Re: Mrdbrewer]
#19328332 - 12/26/13 10:56 AM (10 years, 1 month ago) |
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Quote:
Mrdbrewer said: maybe change the substrate supplier????
I grew them out clean on agar. What would changing a supplier accomplish?
No disrespect intended but some of these have been grown out 5 or 6 times.
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: bootster]
#19328342 - 12/26/13 10:58 AM (10 years, 1 month ago) |
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Quote:
bootster said: I always do that and they remain clean. I have 18 jars of different strains/species and it always shows up at once and soon they will have a powdery look to them. After a shake, the green comes out and forms a green cloud in the jar. Some of these are directly injected by a syringe from a vendor with a red hot needle into an RTV injection port. 
Most come from clean looking agar cultures.
I've had vendor syringes that contaminated everything with mold, but if you're getting green also when you inoculate with agar wedges and the jars you leave closed after the PC don't get mold in them, then it's clearly something to do with your sterile technique. Do you use an SAB or a flow hood?
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bootster


Registered: 02/22/11 
Posts: 1,531
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I am looking at clean mycelium.

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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Why does this happen to all my jars? [Re: bootster]
#19328433 - 12/26/13 11:23 AM (10 years, 1 month ago) |
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Maybe it's like these guys are saying...
something in your grain 
Have you tried pc'ing some WBS n using that since you've been having problems?
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: PirateSwazey]
#19328464 - 12/26/13 11:35 AM (10 years, 1 month ago) |
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Quote:
PirateSwazey said: Maybe it's like these guys are saying...
something in your grain 
Have you tried pc'ing some WBS n using that since you've been having problems?
If you can work agar without contams it's not your sterile technique, are you sure you've tried leaving a few sterilized jars that have been PC'd without innoculating them to test to see if they come out properly sterilized? Are you soaking 24 hours, are you adding gypsum? How long do you PC? Switch to a different source for grain, give it a good long soak, don't add any supplements at first and give it a good solid 90 minutes at 15 PSI. I think it's your grain even though you claim to leave test jars. Might be your gypsum as I've had mold like that the few times I left a bit too much residual gypsum on the grains before PC-ing them. 
If it's not your grain then go on to the next step, which is finding out where you're fucking up your sterile technique.
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bootster


Registered: 02/22/11 
Posts: 1,531
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Re: Why does this happen to all my jars? [Re: bootster]
#19328478 - 12/26/13 11:41 AM (10 years, 1 month ago) |
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I just called my supplier and he said that the rye was absolutely not treated.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Why does this happen to all my jars? [Re: PirateSwazey]
#19328479 - 12/26/13 11:41 AM (10 years, 1 month ago) |
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What kind of filter are you using? What are your procedures for sterilization? How confident are you in transferring agar to grain? It's not hard, but it is kinda tricky to learn to do it just right.
I doubt it's your grain source. That just sounds kind of retarded. All grain is grown outside, stored in a silo or barn and is covered in all kinds of contams.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Juicin
Stranger

Registered: 10/27/13
Posts: 897
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Re: Why does this happen to all my jars? [Re: 36fuckin5]
#19328534 - 12/26/13 11:55 AM (10 years, 1 month ago) |
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Grain supply being tainted for whatever reason makes a lot more sense than a 100% failure rate from agar to grain.
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bootster


Registered: 02/22/11 
Posts: 1,531
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Re: Why does this happen to all my jars? [Re: bootster]
#19328538 - 12/26/13 11:56 AM (10 years, 1 month ago) |
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I've never had any problems before and it's been going on for a long time now. I am spending way too much $$$ just to see fruits.
I use a SAB of my own design. I've done many monotubs before. I've grown pounds of Shiitake, Blue Oyster, King Oyster, Lions Mane and many other kinds of edibles using two separate suppliers. I've never had a problem. I'm using Prof P's lid TEK (before he started to demean me) with a 3/8" hole stuffed with poly fill and aluminum caps.
I'm thinking that the grain is too dry. I went on a bizarre run for a while when the grains were too wet and had tons of bacteria issues.
I just don't get it, unless the Tyvek sleeves in my SAB bring contams into it.
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Why does this happen to all my jars? [Re: bootster]
#19328558 - 12/26/13 12:04 PM (10 years, 1 month ago) |
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Your filter is probably your problem. I'm having the same issue. Polyfil just isn't that great unless your environment is fairly clean.
Get some SFDs from Out-Grow or Aloha (the stupidly thick ones, like 1/16"), use them whole as intended and check your house thoroughly for mold.
You also didn't answer my question about your sterilization procedure. I need every detail, too.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Why does this happen to all my jars? [Re: 36fuckin5]
#19328584 - 12/26/13 12:12 PM (10 years, 1 month ago) |
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might be a problem in the sterilization process not enough pressure in the PC?
i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
-------------------- SPREAD THE SPORES
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Why does this happen to all my jars? [Re: Aero]
#19328590 - 12/26/13 12:14 PM (10 years, 1 month ago) |
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Quote:
Aero said: i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
WTF would this teach you about this particular problem?
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 3 months
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Re: Why does this happen to all my jars? [Re: 36fuckin5]
#19328598 - 12/26/13 12:16 PM (10 years, 1 month ago) |
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Quote:
36fuckin5 said:
Quote:
Aero said: i would do a few verm+whole brown rice jars to see whats the problem
2part verm 1 part rice 1part water
WTF would this teach you about this particular problem?
that the culture+syringe+environment is clean my friend, and the problem is the grain
think before u start raging
-------------------- SPREAD THE SPORES
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 6 months
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Re: Why does this happen to all my jars? [Re: Aero]
#19328605 - 12/26/13 12:19 PM (10 years, 1 month ago) |
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Checking the filters is a good idea. What tek are you using for your grain jar lids?
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