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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 5 months
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Re: Why does this happen to all my jars? [Re: cronicr]
#19329769 - 12/26/13 05:26 PM (10 years, 1 month ago) |
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Quote:
cronicr said: as long as he hydrates right the grain choice won't matter When the grains are hydrated, the hard shell of the endospore is also hydrated and will be killed in the pc weather or not it germinated
All I'm saying is the first thing I told OP was to make sure his grain prep is solid.
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
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Re: Why does this happen to all my jars? [Re: cronicr]
#19329849 - 12/26/13 05:50 PM (10 years, 1 month ago) |
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Quote:
cronicr said: as long as he hydrates right the grain choice won't matter When the grains are hydrated, the hard shell of the endospore is also hydrated and will be killed in the pc weather or not it germinated
oops that was a rr qoute i just forgot to qoute instead i just copied
-------------------- SPREAD THE SPORES
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 9 hours, 51 minutes
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Re: Why does this happen to all my jars? [Re: PirateSwazey]
#19330275 - 12/26/13 07:42 PM (10 years, 1 month ago) |
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Quote:
PirateSwazey said:
Quote:
jellyfish said: I know it's hard to tell but those grains don't look hydrated to me. I'd make sure your pressure cooker is maintaining the correct temperature. Remember, agar will take a lot less time to sterilize then grain.
That is a good point. I don't think anyone else has offered the PC being an issue here 
If it was bacteria perhaps since some are very heat resistant but it's not realistic for sterilization to be the cause of mold in a spawn jar.
It wouldn't be the grain prep either. Granted some molds can only grow on damaged grains in any case it would still mean mold is getting into the jar somehow after sterilization.
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Why does this happen to all my jars? [Re: Kizzle]
#19330634 - 12/26/13 09:09 PM (10 years, 1 month ago) |
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you're the contam major kizzle not me 
I thought it was bacterial
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ChaostoOrder
Lakota Peji Wicasa,



Registered: 08/01/09
Posts: 553
Last seen: 1 year, 1 month
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Re: Why does this happen to all my jars? [Re: 36fuckin5]
#19330659 - 12/26/13 09:15 PM (10 years, 1 month ago) |
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For one your rye looks wayyy to dry. Boiling them after a 12 hour soak is a good way to add water to the rye. Its not necessarily a way to boil out bacteria, because we all know you will never fully rid them of that...but yeah you should boil your rye after your soak. That mycellium looks like its roughin it bro... is that some trich I see above your mycellium????
-------------------- Too weird to live, to rare to die....-Hunter S. Thompson

: [url=https://files.shroomery.org/files/10-21/47399604
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Why does this happen to all my jars? [Re: ChaostoOrder]
#19330685 - 12/26/13 09:21 PM (10 years, 1 month ago) |
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Contamination need to be introduced to sterile media. If a "test" jar is left uninoculated and does not contaminate, than it must be the method of introducing a culture OR the culture itself.
The water density of sterile media has no impact on mold growing. If mold is growing, I would deduce that water content is close to correct.
If this is happening EVERY time, it is time to clean house. Get new prints and start from square one. It can happen.
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RiparianZoneJunky
hunter/gatherer



Registered: 10/30/11
Posts: 3,055
Loc: Oregon
Last seen: 3 years, 5 months
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Let us know what you end up doing and how it works out OP, seems to me you have a wealth of options to try. I still think it's either your grain prep or your filter, I do too much agar to buy the idea that there are hidden contams on your cultures that only grow when you put them on grain, you clearly have enough sense to make a transfer without growing green on agar and that's way harder than noccing jars.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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try a better filter and report back after using your same cultures
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