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jbeeebz
Stranger

Registered: 12/09/13
Posts: 24
Loc: New Jersey
Last seen: 9 years, 10 months
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8 days since innoc. no sign of germination :(
#19328001 - 12/26/13 08:51 AM (10 years, 1 month ago) |
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Hello. I inoculated my jars following the pf tek to the t. They are in an area that is constantly 73 degrees with maybe one degree above or below in fluctuation. Humidity in the room is 40%. They are the colom rust spore strain. It has been 8 days since inoculation and I still see no signs of germination. As of now they are not receiving any light. Will more light help trigger germination? I have aluminum foil covering the bin they are in.I had left the foil loosely covering the top to allow fae but I read somewhere that I might need a co2 buildup, so should I cover the bin tighter?
Edit: brf cakes in half pint jars
Edited by jbeeebz (12/26/13 10:27 AM)
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indocult
Dr


Registered: 07/12/09
Posts: 1,395
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Re: 8 days since innoc. no sign of germination :( [Re: jbeeebz]
#19328345 - 12/26/13 10:58 AM (10 years, 1 month ago) |
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Did you shake the syringe before each jar ? Syringe from vendor? Did you allow the jars to cool long enough before inoculation?
Still 8 days is not too bad sometimes it takes a while.
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jbeeebz
Stranger

Registered: 12/09/13
Posts: 24
Loc: New Jersey
Last seen: 9 years, 10 months
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Re: 8 days since innoc. no sign of germination :( [Re: indocult]
#19328348 - 12/26/13 11:00 AM (10 years, 1 month ago) |
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yes i shook very well. they are from sporeworks. i allowed the jars to cool for almost 24 hours. I am probably just being paranoid.. i just want to make sure I did not do anything wrong. should i be fanning co2 out or just leave it be?
I forgot to mention that i have 4 points of inoculation per jar. 1/4 cc per hole. I heard multiple point injection can take longer?
Edited by jbeeebz (12/26/13 11:03 AM)
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lighthouse09
Stranger thats mr. stranger



Registered: 03/16/13
Posts: 699
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Re: 8 days since innoc. no sign of germination :( [Re: indocult]
#19328356 - 12/26/13 11:02 AM (10 years, 1 month ago) |
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not weird at all we get a post like this several times a week it can take up to two weeeks sometimes more still be patient and chck em every few days .Picking up and shaking and looking every day will slow it down evenmore. just let it be they got air holes right?
--------------------
<--This fuckin guy
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jbeeebz
Stranger

Registered: 12/09/13
Posts: 24
Loc: New Jersey
Last seen: 9 years, 10 months
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Re: 8 days since innoc. no sign of germination :( [Re: lighthouse09]
#19328367 - 12/26/13 11:05 AM (10 years, 1 month ago) |
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i am making sure not to actually touch the chamber. all i am doing is shining a flashlight through the side to inspect. the only air holes are the injection points. is that enough? or do i need holes in the chamber as well?
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: 8 days since innoc. no sign of germination :( [Re: jbeeebz]
#19328371 - 12/26/13 11:05 AM (10 years, 1 month ago) |
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just leave it and be patient. Like Indocult said 8 days isn't that long.
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jbeeebz
Stranger


Registered: 12/09/13
Posts: 24
Loc: New Jersey
Last seen: 9 years, 10 months
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Re: 8 days since innoc. no sign of germination :( [Re: MudaFuka]
#19328570 - 12/26/13 12:08 PM (10 years, 1 month ago) |
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well now i feel stupid because i just checked it for the first time today and I now see that each jar (i have 7) have small germination signs at each point of innoculaton. i needed a super close look without a glare from my flashlight and its obviuos i have germination. YAY!
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: 8 days since innoc. no sign of germination :( [Re: jbeeebz]
#19329691 - 12/26/13 05:02 PM (10 years, 1 month ago) |
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right on glad to hear it.
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indocult
Dr


Registered: 07/12/09
Posts: 1,395
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Re: 8 days since innoc. no sign of germination :( [Re: MudaFuka]
#19329976 - 12/26/13 06:22 PM (10 years, 1 month ago) |
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Lol well better to feel stupid than have contams or some other issue and not ask ?'sI
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