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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 7 months
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Just Got a Scope! My First Sections...
#19326824 - 12/25/13 08:39 PM (10 years, 1 month ago) |
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This was the first one I cut. Pleurotus ostreatus
Next, Psilocybe caerulescens var. weilii pleurocystidia. I am going to rehydrate and try and section a piece of a more "var. caerulescens" fruit. We'll see if there are pleurocystidia as well. I say hell yes.
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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Roger Wilco
Rusted Identifier

Registered: 06/08/13
Posts: 970
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19326927 - 12/25/13 09:12 PM (10 years, 1 month ago) |
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Good stuff! I'm excited for you.
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TimmiT


Registered: 03/23/10
Posts: 5,303
Loc: Victoria
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19327072 - 12/25/13 10:07 PM (10 years, 1 month ago) |
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Have you mounted them in anything or are you looking at them dry? If it's the latter try putting a drop of water on your section and then a cover slip over the top.
-------------------- "Reality leaves a lot to the imagination" ~ John Lennon
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Anglerfish
hearing things



Registered: 09/08/10
Posts: 18,644
Loc: Norvegr
Last seen: 2 hours, 57 minutes
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19327615 - 12/26/13 04:12 AM (10 years, 1 month ago) |
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Congratulations! 
Please keep us posted on this.
--------------------
★★★★★
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 7 months
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Re: Just Got a Scope! My First Sections... [Re: TimmiT]
#19327984 - 12/26/13 08:44 AM (10 years, 1 month ago) |
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Quote:
TimmiT said: Have you mounted them in anything or are you looking at them dry? If it's the latter try putting a drop of water on your section and then a cover slip over the top.
Yes I have them on slides. The oyster, I thinly sliced a piece of one gill, so its like a gill cut down the middle kind of on an angle. I "fileted" it. The caerulescens was really difficult because I had to rehydrate the piece of dried cap. I've made a few conclusions... Fresh material is better than dried, and LBM's are fucking difficult as shit to slice thinly. I give mad props to all you guys busting your balls scoping tiny little LBM's. Its hard, but so much fun! Its like another world within this hobby.
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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bloodworm
cube con·nois·seur


Registered: 05/22/10
Posts: 10,926
Loc: 352
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19328677 - 12/26/13 12:40 PM (10 years, 1 month ago) |
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art2312
wanderer



Registered: 07/08/13
Posts: 3,352
Loc: The land, Ohio
Last seen: 1 year, 9 months
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Re: Just Got a Scope! My First Sections... [Re: bloodworm]
#19328979 - 12/26/13 01:50 PM (10 years, 1 month ago) |
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Damn, that's sweet! Congrats bro! Now you can scope all your sic finds!! Can't wait
-------------------- I don't mind being ogled, ridiculed, made to feel minuscule. If you consider the source, it's kinda pitiful The only thing you really know about me is.....That's all you'll ever know!!!!
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 7 months
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Re: Just Got a Scope! My First Sections... [Re: bloodworm]
#19329187 - 12/26/13 02:44 PM (10 years, 1 month ago) |
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-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19329554 - 12/26/13 04:31 PM (10 years, 1 month ago) |
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Welcome to the club man! I'm still not very good at using this thing even though I got it a year ago. Practice makes permanent, so keep it up. I'm happy for you, merry Christmas  I just got a stage micrometer for Christmas this year, so I'll actually can measure the things that I see.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Quote:
maynardjameskeenan said:
Welcome to the club man! I'm still not very good at using this thing even though I got it a year ago. Practice makes permanent, so keep it up. I'm happy for you, merry Christmas  I just got a stage micrometer for Christmas this year, so I'll actually can measure the things that I see.
Nice, I have one too, you can't actually measure anything with it, you only use it to calibrate a reticle that is fitted into one of the eyepieces!
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 7 months
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Re: Just Got a Scope! My First Sections... [Re: inski]
#19330656 - 12/26/13 09:15 PM (10 years, 1 month ago) |
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Quote:
inski said:
Quote:
maynardjameskeenan said:
Welcome to the club man! I'm still not very good at using this thing even though I got it a year ago. Practice makes permanent, so keep it up. I'm happy for you, merry Christmas  I just got a stage micrometer for Christmas this year, so I'll actually can measure the things that I see.
Nice, I have one too, you can't actually measure anything with it, you only use it to calibrate a reticle that is fitted into one of the eyepieces!
So how does one go about measuring spores and such?
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19330765 - 12/26/13 09:46 PM (10 years, 1 month ago) |
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Quote:
Hashfinger said:
Quote:
inski said:
Quote:
maynardjameskeenan said:
Welcome to the club man! I'm still not very good at using this thing even though I got it a year ago. Practice makes permanent, so keep it up. I'm happy for you, merry Christmas  I just got a stage micrometer for Christmas this year, so I'll actually can measure the things that I see.
Nice, I have one too, you can't actually measure anything with it, you only use it to calibrate a reticle that is fitted into one of the eyepieces!
So how does one go about measuring spores and such?
You need a reticle which is fitted into one of the eyepieces, the reticle needs to be calibrated using a stage micrometer.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,276
Last seen: 10 hours, 8 minutes
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19330906 - 12/26/13 10:31 PM (10 years, 1 month ago) |
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Quote:
Hashfinger said: So how does one go about measuring spores and such?
The best way is to use a camera to take a photo of a stage micrometer, and then use that photo to calibrate software like Piximetre. Then you can measure lots of spores quickly and it automatically calculates spore statistics and stuff.
You can also use a reticle, but that costs money and is a lot slower.
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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I used a similar method to create scales to overlay onto my micrographs using Gimp. Some examples here. http://mushroomobserver.org/55302?q=1huQD http://mushroomobserver.org/50379?q=1huQi
I see no problems with your method Alan as long as it is a properly mounted camera, if one is lining a camera up to one of the eyepieces or using any other unconventional or makeshift method whereby it's possible that every time the camera is set up it is not exactly as it was when the calibration photo was taken the measurements made with your software will be out and because we are talking increments often as small as 1µm the slightest difference in how the camera is mounted could affect your measurements drastically.
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Hashfinger
Nippy Wiffle



Registered: 07/10/12
Posts: 4,775
Loc: Georgia
Last seen: 5 years, 7 months
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Re: Just Got a Scope! My First Sections... [Re: inski]
#19332066 - 12/27/13 08:04 AM (10 years, 1 month ago) |
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Quote:
inski said: I used a similar method to create scales to overlay onto my micrographs using Gimp. Some examples here. http://mushroomobserver.org/55302?q=1huQD http://mushroomobserver.org/50379?q=1huQi
I see no problems with your method Alan as long as it is a properly mounted camera, if one is lining a camera up to one of the eyepieces or using any other unconventional or makeshift method whereby it's possible that every time the camera is set up it is not exactly as it was when the calibration photo was taken the measurements made with your software will be out and because we are talking increments often as small as 1µm the slightest difference in how the camera is mounted could affect your measurements drastically.
So yes because I am shooting down the lens with my camera manually, should I just go with the stage micrometer and reticle? Sounds like this is the more time-consuming, yet sure-fire way to get an accurate measurement.
-------------------- Species List (Georgia): Psilocybe caerulescens/weilii, Psilocybe atlantis/galindoi, Psilocybe cubensis, Psilocybe ovoideocystidiata, Psilocybe caerulipes, Psilocybe semilanceata, Psilocybe fagicola, Copelandia cyanescens, Panaeolus cinctulus, Panaeolus fimicola, Panaeolus olivaceus, Gymnopilus luteofolius, Gymnopilus aeruginosus, Gymnopilus junonius, Pluteus salicinus (Ohio): Psilocybe ovoideocystidiata, Psilocybe caerulipes, Pluteus cyanopus, Pluteus salicinus sensu lato..., Panaeolus cinctulus, Gymnopilus luteus, Gymnopilus luteofolius, Gymnopilus junonius, Gymnopilus aeruginosus
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19332268 - 12/27/13 09:29 AM (10 years, 1 month ago) |
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Quote:
Hashfinger said: So yes because I am shooting down the lens with my camera manually, should I just go with the stage micrometer and reticle? Sounds like this is the more time-consuming, yet sure-fire way to get an accurate measurement.
Its not that much slower, you can measure ~20 spores in 10-15 minutes. The time intensive part is doing all the math.
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Just Got a Scope! My First Sections... [Re: Hashfinger]
#19332283 - 12/27/13 09:33 AM (10 years, 1 month ago) |
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You might consider getting a USB microscope camera and never adjust its position once you install it properly.
If you do adjust it, just go thru the recalibration process again. You should do that every now 'n' then to be sure, regardless.
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Just Got a Scope! My First Sections... [Re: The Lightning]
#19332350 - 12/27/13 09:56 AM (10 years, 1 month ago) |
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Have any of you ever tried comparing measurements made by hand to those made by the program? I would think that often you will need to adjust the focus by hand to see the entire cell and measure it accurately. This is especially true with cystidia.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
Alan Rockefeller said:
Quote:
Hashfinger said: So how does one go about measuring spores and such?
The best way is to use a camera to take a photo of a stage micrometer, and then use that photo to calibrate software like Piximetre. Then you can measure lots of spores quickly and it automatically calculates spore statistics and stuff.
You can also use a reticle, but that costs money and is a lot slower.
This^ makes things so much better in the end.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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