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Offlinebuddhahoodlum
Bodhisattva
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Registered: 04/30/07
Posts: 354
Loc: Buddhahood
Last seen: 3 years, 6 months
Re: Cactus tissue culture. [Re: Entersandman]
    #6990962 - 05/31/07 11:10 AM (16 years, 7 months ago)

where do you get these plant tubes?
http://www.shroomery.org/forums/files/080503-46/72157-pereskiopsis.jpg
...and what are they called?


Doe's anyone know the answer to this question?


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“I believe that water will one day be employed as fuel, that hydrogen and oxygen which constitute it, used singly or together, will furnish an inexhaustible source of heat and light, of an intensity of which coal is not capable.” – Jules Verne, T

“Ere many generations pass, our machinery will be driven by power obtainable at any point in the universe...it is a mere question of time when men will succeed in attaching their machinery to the very wheel-work of nature."
- Nikola Tesla



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Invisiblethallus
Stranger thanyou

Registered: 08/15/06
Posts: 629
Loc: Hiding in Plain Sight
Re: Cactus tissue culture. [Re: buddhahoodlum]
    #6991686 - 05/31/07 02:02 PM (16 years, 7 months ago)

Good read:thumbup:


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OfflineFreeSporePrints
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Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Cactus tissue culture. [Re: Una]
    #8391525 - 05/12/08 02:27 AM (15 years, 8 months ago)

Hi mates,
i hope you can be interested to give a hand with our micropropagation project:

http://www.shroomery.org/forums/showflat.php?Cat=0&Number=8391485&page=0&vc=1&PHPSESSID=#Post8391485

Bye,
Fabio


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Offlineroyer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
Re: Cactus tissue culture. [Re: FreeSporePrints]
    #8395641 - 05/13/08 07:27 AM (15 years, 8 months ago)

please do not dig up old threads to plug a thread you have already started!!


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if you have any questions please feel free to pm me , thx :-)


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Offlineroyer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
Re: Cactus tissue culture. [Re: Una]
    #8395642 - 05/13/08 07:27 AM (15 years, 8 months ago)

i am going to leave this thread open due to good info but if you are going to use it to plug your thread then i will re-lock it.


Edited by royer9864 (05/13/08 07:29 AM)


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OfflineFreeSporePrints
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Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Cactus tissue culture. [Re: royer]
    #8395783 - 05/13/08 08:41 AM (15 years, 8 months ago)

Hi royer9864,
i've added the link to link this thread with the other project. I believe a lot of people here are interested in the micropropagation project, and i don't earn anything doing this..i simply do this for the community..so gently next time think if a thing made can be useful for the users or not and in this case i believe yes :smile:

Nobody against you,
Fabio


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Offlineroyer
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Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
Re: Cactus tissue culture. [Re: royer]
    #8395849 - 05/13/08 09:08 AM (15 years, 8 months ago)

Quote:

royer9864 said:
i am going to leave this thread open due to good info but if you are going to use it to plug your thread then i will re-lock it.




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if you have any questions please feel free to pm me , thx :-)


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OfflineFreeSporePrints
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Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
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Re: Cactus tissue culture. [Re: royer]
    #8437142 - 05/23/08 02:30 PM (15 years, 8 months ago)

Thanks for stopping micropropagation to micropropagate users!

Fabio


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Offlineroyer
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Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
Re: Cactus tissue culture. [Re: FreeSporePrints]
    #8439423 - 05/24/08 04:58 AM (15 years, 8 months ago)

i did not stop anything, have a nice day


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if you have any questions please feel free to pm me , thx :-)


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OfflineFreeSporePrints
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Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
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Re: Cactus tissue culture. [Re: Una]
    #10433281 - 06/01/09 12:11 AM (14 years, 7 months ago)

Quote:

Una said:
I can not reveal the recipe but if you are interested in cactus tissue culture this document will give some very usefull tips.

http://plant-tc.coafes.umn.edu/listserv/2000/log0010/msg00077.html





Clicking on the link of Una doesn't work actually, but this works!

http://plant-tc.cfans.umn.edu/listserv/2000/log0010/msg00077.html

I quote also the text:

Quote:

    * Subject: cacti recepy
    * From: "R. Wellens" <rwellens@ZEELANDNET.NL>
    * Date: Tue, 10 Oct 2000 19:43:42 +0200
    * Reply-To: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>
    * Sender: Plant Tissue Culture <PLANT-TC@TC.UMN.EDU>

Dear Diana,

There is no such thing as a cactus recepy; there is a huge diversity within
the cactaceae and almost each different one asks for a different approach.
In general (very general!) most species will grow on a 1/2 MS or 1/2 B5
medium supplemented with BA in concentrations varying from 0.1 to 10 mg/l.
Some cacti are extremely hard to propagate, for instance the cold-hardy ones
from North-America (Sclerocactus, Pediocactus etc.). So are cacti like
Ariocarpus, Aztekium etc, the slow growers from Mexico.
Relatively easy are most (not all!!) of the Rebutia's, Mammillaria's,
Gymnocalycium's etc.
Normally they are multiplied using areole activation. This can be done on
the medium described above and is sometimes enhanced by culture in the dark
for 2-3 weeks.
You can take pieces of cactus with at least 3-4 areoles and cultivate them
in the dark for 3 weeks on the described medium.
When you cannot start from a sterile (seedling) culture and must start with
an adult plant you will find it hard to desinfest. Normally if that happens
we decide to cut off the biggest spines and sterilize only the top meristem.
We use a 2% bleach for a relatively long time (1 hour or more) and shake the
culture very frequently. Sometimes we add an dropp of dish-washingsoap to
this or you can use a drop of Tween. You still might find fungal infections,
even after a long sterilization. But once you have a single clean cactus you
can easily start the multiplicationprocess with areole activation, although
you the hard-to-do ones mentioned above will ask a lot of time and effort to do so.
Rooting is mostly not a big problem, but again, it is a problem for some species.
Rooting for easy species happens on plain 1/2MS without any auxins, but in
individual cases an auxin like IBA might be helpful.
A main problem in producing cati is hyperhydricity or vitrification.
This can be reduced by using vessels or jars that have air-filtering
capacity, not to much because then they dry out.
So once you know what type of cactus you are going to multiply you will have
to develop its own perfect protocol, which can be based on this information.
However, it is not complete, there are a few articles in which specific
cacti were produced with specific techniques.
Callus culture or somatic embryogenesis is so far not studied by us and is
scarcely found in literature with little success.
I hope this will help you, you can also see some literature on different species.
Success with your productions.

Robert Wellens
Succulent Tissue Culture





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OfflineFreeSporePrints
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Registered: 03/06/05
Posts: 1,139
Loc: Rome, Italy Flag
Last seen: 1 month, 26 days
Re: Cactus tissue culture. [Re: FreeSporePrints]
    #12547466 - 05/12/10 08:22 AM (13 years, 8 months ago)

Nobody tried with tissue culture?

Fabio


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OfflineOzzy_shroomer
Mystery Machine
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Registered: 02/10/08
Posts: 911
Loc: Summer All Year
Last seen: 5 years, 7 months
Re: Cactus tissue culture. [Re: FreeSporePrints]
    #12558609 - 05/13/10 09:46 PM (13 years, 8 months ago)

Sorry i hate doing this to old threads but found this a great resouce also have somthing to add and have the PDF if anyone wants it

Inoculation
Explants were cultured in culture bottles containing MS (Murashige and Skoog, 1962) basal media supplemented
with benzyladenine (BA) (0.5, 1.0, 1.5, 3.0 and 5.0 mg L-1) or Kinetin (Kin) (0.5, 1.0, 1.5, 3.0 and 5.0 mg L-1) alone
or in combination with naphthaleneacetic acid (NAA) (0.5 mg L-1). All media were prepared by standard procedures
and the culture bottles were transferred to growth chamber with a photoperiod of 16 h light and 8 h of dark at 25ºC ±
2 ºC and incubated for 3 months


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InvisibleBig L
In tall buildings
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Registered: 02/11/09
Posts: 3,532
Loc: Luxury
Re: Cactus tissue culture. [Re: Ozzy_shroomer]
    #12558903 - 05/13/10 10:30 PM (13 years, 8 months ago)

Nice bump.

I fully support bumping any of Una's threads.
Una is the bees knees.


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OfflinetheMallacht
Clandestine Hero


Registered: 04/25/09
Posts: 3,428
Last seen: 1 year, 30 days
Re: Cactus tissue culture. [Re: Big L]
    #12558938 - 05/13/10 10:34 PM (13 years, 8 months ago)

The bees knees INDEED!

This thread is great... I clicked and just started reading the entirety of the OP, was SUPER impressed and immediately wanted to post and tell Una how awesome he is and ask about 10 questions, then I realized that it was a 2 yr old thread.


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InvisibleBig L
In tall buildings
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Registered: 02/11/09
Posts: 3,532
Loc: Luxury
Re: Cactus tissue culture. [Re: theMallacht]
    #12558965 - 05/13/10 10:38 PM (13 years, 8 months ago)

I highly highly recommend viewing Una's "Main threads" list.


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OfflinePsychdelicSpore
Funginner
Male

Registered: 08/08/22
Posts: 13
Last seen: 1 month, 4 days
Re: Cactus tissue culture. [Re: Una] * 1
    #28084253 - 12/05/22 05:36 AM (1 year, 1 month ago)

Why post BS and not tell people how to make it? Why bring it up? This is a Forum! That means don't BS people a bring up something that doesn't matter! How about delete this whole post because it doesn't teach anyone 💩! Stop posting crap that has no info on a DIY. You help no one and post crap to make yourself feel better. 😆😂🤣 GTFOH!


Edited by PsychdelicSpore (12/05/22 05:38 AM)


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