Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2107117 - 11/14/03 04:47 PM (20 years, 2 months ago)

Ok here's a good pic of the san pedro. Most all of the areoles are swelling and sprouting new shoots. It's more than a week faster than the initial explants reacted. The plants are adapting to their new environment :smile:



And some lophophora williamsii.




 


--------------------
www.911blogger.com


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2135231 - 11/25/03 02:40 PM (20 years, 2 months ago)

just a little update:



--------------------
www.911blogger.com


Extras: Filter Print Post Top
OfflinestonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666 666 Posts!
Last seen: 13 years, 10 months
Re: Cactus tissue culture. [Re: Una]
    #2135247 - 11/25/03 02:50 PM (20 years, 2 months ago)

Keep em comming Una..this is sooo cool.
Looking forward to more updates..

peace..


Extras: Filter Print Post Top
Invisiblemedicinebag
Hunting
Registered: 11/15/03
Posts: 344
Loc: The land of The People
Re: Cactus tissue culture. [Re: stonErollEr1]
    #2136493 - 11/25/03 11:23 PM (20 years, 2 months ago)

What kind of Base is the culture setting in?


Extras: Filter Print Post Top
Offlineneuro
Phytophiliac
 User Gallery

Registered: 08/10/99
Posts: 6,633
Loc: Rigel 7
Last seen: 2 months, 15 days
Re: Cactus tissue culture. [Re: medicinebag]
    #2136592 - 11/26/03 12:09 AM (20 years, 2 months ago)

the explants are on agar with nutrition and hormones


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2227778 - 01/08/04 08:53 AM (20 years, 25 days ago)

These pic were taken a few weeks ago. The pachanoi is growing very nice in tissue culture.

Group photo



Close-up



Loph. williamsii callus cutlure with spontaneous button formation.



Their habitat :wink:



--------------------
www.911blogger.com


Extras: Filter Print Post Top
InvisibleDazedSol
old hand

Registered: 08/01/01
Posts: 1,230
Re: Cactus tissue culture. [Re: Una]
    #2229031 - 01/08/04 05:14 PM (20 years, 24 days ago)

Very very nice!!!

Wish i had time to experiment more.........ever try tissue culture of cannabis??


--------------------
Peace,
Adam


Extras: Filter Print Post Top
OfflineOpenminded
Dicotyledon

Registered: 08/28/03
Posts: 657
Loc: England.
Last seen: 6 years, 29 days
Re: Cactus tissue culture. [Re: DazedSol]
    #2230450 - 01/09/04 05:46 AM (20 years, 24 days ago)

For anyone that's interested in tissue culture, this page:

http://www.oup.co.uk/pdf/bt/slater/ch02.pdf

Might be a good starting point. It gives the composition of a typical culture medium, briefly describes the roles of the different growth regulators, gives examples of them, etc.

Edit: the ratios etc that they give surely won't be optimised for cacti, but I still think it's a page that is worth reading...


Edited by Openminded (01/09/04 05:50 AM)


Extras: Filter Print Post Top
Offlineangryjslice
now with 20%more anger
Male

Folding@home Statistics
Registered: 10/29/03
Posts: 916
Last seen: 4 years, 23 days
Re: Cactus tissue culture. [Re: Una]
    #2267795 - 01/24/04 07:56 AM (20 years, 9 days ago)

bump/question
this thread is amazing

una, do you forsee any problems in transfering these back out of the liquid medium there in into soil?

~JSlice~


--------------------


Extras: Filter Print Post Top
Offlineneuro
Phytophiliac
 User Gallery

Registered: 08/10/99
Posts: 6,633
Loc: Rigel 7
Last seen: 2 months, 15 days
Re: Cactus tissue culture. [Re: angryjslice]
    #2268288 - 01/24/04 01:09 PM (20 years, 9 days ago)

There should be no problems what so ever. In a class i took some time ago, we got these african violets with chimera's on them. We isolated the tissue that was exhibiting the chimera and grew out african violets on the culture then transfered back to soil. No problems. Unfortunately the chimera's didn't last and the violets turned green again after some time.


Extras: Filter Print Post Top
OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 6 months
Re: Cactus tissue culture. [Re: neuro]
    #2935660 - 07/28/04 08:10 AM (19 years, 6 months ago)

Is it possible to grow Salvia the same way?
What kind of media should I use then?

YSr


Extras: Filter Print Post Top
Offlineneuro
Phytophiliac
 User Gallery

Registered: 08/10/99
Posts: 6,633
Loc: Rigel 7
Last seen: 2 months, 15 days
Re: Cactus tissue culture. [Re: YSr]
    #2936577 - 07/28/04 02:15 PM (19 years, 6 months ago)

>>Is it possible to grow Salvia the same way?

Yes.

>>What kind of media should I use then?

Agar as the mediu, and proper phytonutrients and most important hormones. Plant Tissue culture is quite a complex thing compared average horticulture so much so that most colleges devote whole classes usually 101/102 and upper level tissue culture 300.

I suggest getting a book and startting from there and also a good supplier of IAA, GA, and the like. Because it all depends on the ratio of plant hormones as to whether you get roots, shoots, or both, or a callus .


Extras: Filter Print Post Top
InvisibleStonehenge
Alt Center
Male User Gallery
Registered: 06/20/04
Posts: 14,850
Loc: S.E.
Re: Cactus tissue culture. [Re: neuro]
    #2936801 - 07/28/04 03:02 PM (19 years, 6 months ago)

I think I'd like to take one of those classes. Another one I'd like to take is photoshop. The photoshop tutorials are just about worthless. I'll check to see if any tissue culture classes are offered in my area but I doubt it.


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: YSr]
    #2936954 - 07/28/04 03:39 PM (19 years, 6 months ago)

Salvia can be grown in tissue culture quite easily. But.....my medium recipe is a secret, that's the way things are in tissue culture land...No one shares their recipes and it's often a long trial and error process to get some decent growth.

Here are two pics...

This plantlet has just started to root...


These guys are ready to be transplanted to soil.


--------------------
www.911blogger.com


Extras: Filter Print Post Top
OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 6 months
Re: Cactus tissue culture. [Re: Una]
    #2939467 - 07/29/04 05:30 AM (19 years, 6 months ago)

But could you please give a light introduction of some ingredients
to start experimenting with?

And please give a short description of what IAA and GA is....

YSr


Edited by YSr (07/29/04 05:32 AM)


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: YSr]
    #2939491 - 07/29/04 05:56 AM (19 years, 6 months ago)

IAA and GA3 are plant growth regulators. They signal the plant to development in a certain way; shoots, roots, callus etc.

A small introduction to plant tissue culture can be found here.


--------------------
www.911blogger.com


Extras: Filter Print Post Top
OfflineYSr
Stranger

Registered: 04/13/04
Posts: 81
Last seen: 17 years, 6 months
Re: Cactus tissue culture. [Re: Una]
    #2939507 - 07/29/04 06:18 AM (19 years, 6 months ago)

Do you have some more links to pages where I can read about diffrent
mediums for plant tissue cultures?

I'm very interested and don't know where to start. Love working with
shroom-cultures om agar and liquid karo.

YSr


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: YSr]
    #2939550 - 07/29/04 06:56 AM (19 years, 6 months ago)



--------------------
www.911blogger.com


Extras: Filter Print Post Top
Offlinefaslimy
Dead Man
 User Gallery
Registered: 04/04/04
Posts: 3,436
Last seen: 8 years, 1 month
Re: Cactus tissue culture. [Re: Una]
    #2946673 - 07/30/04 10:28 PM (19 years, 5 months ago)

There is another group of people who decided to keep their findings to themselves, they enslaved humanity for world domination. Why keep everyone in the dark on the best methods for things?


Extras: Filter Print Post Top
Invisibleeric_the_redS
Trans-male User Gallery

Registered: 02/28/03
Posts: 13,742
Loc: happy land
Re: Cactus tissue culture. [Re: faslimy]
    #2946717 - 07/30/04 10:40 PM (19 years, 5 months ago)

i don't see how una's secret formula compares to enslaving humanity in any way. una has a lot of money, time, research, and hard work invested in his business. he is not obligated to give away what he has worked so hard to find. there is nothing wrong with reaping the benefits of your hard work.

una has pointed you in the right direction. if you are really interested, do some research and follow the path that you have been shown.


--------------------
Anno cock? is that some kind of Greek liqueur? -Geo's All Knowing Sex Slave


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]

Shop: PhytoExtractum Buy Bali Kratom Powder   Bridgetown Botanicals Bridgetown Botanicals   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Cacti tissue culture question llamabox 1,616 7 04/22/06 11:37 AM
by whatever123
* Tissue culture tek ?? the man 1,752 2 10/07/04 09:20 PM
by neuro
* Plant Tissue Culture mattymonkey 1,233 1 02/23/07 10:33 AM
by Schwip
* Tissue culture cloning jellyfish 4,463 7 08/25/06 06:24 PM
by karode13
* tissue culture cricket 1,230 4 02/19/05 01:01 PM
by Legoulash
* EZ and Cheap Cloning from Tissue Culture runnerup 3,387 6 12/24/04 01:00 PM
by pod3
* what is the procedure to make salvia tissue cultures? FGL 2,313 12 09/13/06 03:24 PM
by g0pher
* Tissue Culture / Microponics Baby_Hitler 3,270 11 05/19/03 08:32 PM
by neuro

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Mostly_Harmless, A.k.a
51,889 topic views. 2 members, 13 guests and 9 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.007 seconds on 13 queries.