Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleAnand
AutisticShroomer
I'm a teapot User Gallery

Registered: 11/05/02
Posts: 366
Loc: The netherlands
Re: Cactus tissue culture. [Re: Openminded]
    #1943811 - 09/23/03 09:30 AM (20 years, 4 months ago)

Una: will MushMush sell plant Tissue Culture supply's in the future? would be nice.... don't know where to get the MS and other ingredients


Extras: Filter Print Post Top
Invisibletheshiftingwalls
Divine state

Registered: 06/18/03
Posts: 4,128
Loc: Residing in thee Universa...
Re: Cactus tissue culture. [Re: Anand]
    #1946529 - 09/23/03 11:44 PM (20 years, 4 months ago)

He would sell the ingredients to make it possible to culture, get it?
Its all marketing :wink:.



Una you better ship to Canada :wink:! You better!


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: theshiftingwalls]
    #1950405 - 09/25/03 04:39 AM (20 years, 4 months ago)

Embryogenesis from callus is possible with many species so i don't see why it's not possible with cacti. Of course you would need a good protocol (which i don't have yet).

The shoots of the SP took 3 weeks to develop.

We will not be selling plant TC supplies since most of the growth regulators that are needed are toxic (carry the skull icon) and these would require special (expensive) transport.


Anand if you are really serious about this is might help you out with some stuff. Or you might contact :info@labassociates.com at http://www.labassociates.com/



--------------------
www.911blogger.com


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #1957498 - 09/27/03 03:05 PM (20 years, 4 months ago)

I set up some pieces yesterday of this cristate/monstrosus pachanoi.

Here's my flow cabinet. Jars wiith medium and sterilised water on the right. Glass bead heater and more sterilised water on the right:



Surface sterilisation with bleach (toilet cleaner :smile:)



Rinsing with sterile water.



Notice the bleach damage (white tissue)



The damaged tissue is cut off. And the remaining piece is divided.



These pieces are put on the multiplication/initiation medium.



No i cross my fingers and hope some of the areoles develop new buds.


 


--------------------
www.911blogger.com


Extras: Filter Print Post Top
Anonymous

Re: Cactus tissue culture. [Re: Una]
    #1958488 - 09/27/03 10:31 PM (20 years, 4 months ago)

Very interesting stuff. I was talking to my grandmother the other day about growing mushrooms on agar. Then she starts talking about how my greatgrandfather when he was youger got into orchids. He had a full lab and spent all this money on growing thousands of rare orchids. No hybrids. Just pure strains. Then when Hawai came into the picture the market got flooded with orchids. So since he considered himself an artist he took all his plants and equipment and threw them in a pile and burnet them. I wish I had that stuff. Crazy


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #1990544 - 10/08/03 03:50 PM (20 years, 3 months ago)

Here's an update on the San Pedro experiment.

I transferred the pieces to fresh medium and cut away some of the callus. The new shoots are developing steadily:



The Trichocereus bridgesii is now also showing some activity:



--------------------
www.911blogger.com


Extras: Filter Print Post Top
Anonymous

Re: Cactus tissue culture. [Re: neuro]
    #2027654 - 10/21/03 01:33 AM (20 years, 3 months ago)

Callus yields genetic variation.  Bad idea for maintianing cultivars. Good idea for discovering new ones though. 

Stick with the axillary shoot multiplication for maintaining cultivars, while multiplying them. 

Those look nice Una.

Research is very limited in cacti microprop. What is out there is usually not reproducible, important info isconvieniently left out of the methodology. :wink:

I have some journal studies on the subject of cacti microprop. if you are interested.  Explant selection, callus induction, axillary shoot multiplication, somatic embryogenesis, nutritional studies, some specific species studies.  There really wasn't much out there as of 1996.
 


Extras: Filter Print Post Top
Offlineneuro
Phytophiliac
 User Gallery

Registered: 08/10/99
Posts: 6,633
Loc: Rigel 7
Last seen: 2 months, 15 days
Re: Cactus tissue culture. [Re: ]
    #2028100 - 10/21/03 08:11 AM (20 years, 3 months ago)

>>I have some journal studies on the subject of cacti microprop. if you are interested. Explant selection, callus induction, axillary shoot multiplication, somatic embryogenesis, nutritional studies, some specific species studies. There really wasn't much out there as of 1996.

I'd be interested...


Extras: Filter Print Post Top
Anonymous

Re: Cactus tissue culture. [Re: neuro]
    #2029409 - 10/21/03 04:25 PM (20 years, 3 months ago)

PM me an addy. Which studies are you interested in?


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2044680 - 10/26/03 12:37 PM (20 years, 3 months ago)

Lophophora williamsii v. jourdaniana



Trichocereus pachanoi



--------------------
www.911blogger.com


Extras: Filter Print Post Top
InvisibleDazedSol
old hand

Registered: 08/01/01
Posts: 1,230
Re: Cactus tissue culture. [Re: Una]
    #2044693 - 10/26/03 12:42 PM (20 years, 3 months ago)

Hey Una, What kind of lighting do you have those jars under while they grow?


--------------------
Peace,
Adam


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: DazedSol]
    #2046503 - 10/27/03 02:17 AM (20 years, 3 months ago)

They grow under simple fluorescent tubes (33/84 cool white)


--------------------
www.911blogger.com


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2056126 - 10/30/03 02:28 AM (20 years, 3 months ago)

Yesterday i cut up the newly formed shoots. They had been growing for 2 months. Each shoot yielded between 2-4 new sections that were set up on fresh medium. This gave a mean multiplication factor of 7.

If further multiplication goes with that same number in one year 7^5 plants could be obtained. Not 7^6 because the last cycle the plants can not be cut up but should be rooted in a different medium. Still this would mean 16807 plants in one year starting with one explant. Now THAT is rapid multiplication :thumbup:

Cytokinin concentration seems to influence number of shoots and number of ribs per shoot (higher -> more)

Here's two pics:







 


--------------------
www.911blogger.com


Extras: Filter Print Post Top
OfflinestonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666 666 Posts!
Last seen: 13 years, 10 months
Re: Cactus tissue culture. [Re: Una]
    #2056426 - 10/30/03 06:28 AM (20 years, 3 months ago)

And growth would probably be faster if after first initial growth, change lightning into something more powerfull light HPS/MH?[right?]

Peace..


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: stonErollEr1]
    #2056552 - 10/30/03 08:09 AM (20 years, 3 months ago)

Probably not, the atmosphere within the jars does not allow for much assimilation. The medium contains sucrose to supply the plantlets with energy.

The shoots will also develop in the dark but they will remain white/yellow.


--------------------
www.911blogger.com


Extras: Filter Print Post Top
Offlineneuro
Phytophiliac
 User Gallery

Registered: 08/10/99
Posts: 6,633
Loc: Rigel 7
Last seen: 2 months, 15 days
Re: Cactus tissue culture. [Re: Una]
    #2056583 - 10/30/03 08:27 AM (20 years, 3 months ago)

>>If further multiplication goes with that same number in one year 7^5 plants could be obtained. Not 7^6 because the last cycle the plants can not be cut up but should be rooted in a different medium. Still this would mean 16807 plants in one year starting with one explant. Now THAT is rapid multiplication

WOW!  :oogle:


>>Cytokinin concentration seems to influence number of shoots and number of ribs per shoot (higher -> more)

Rather interesting that it affected number of ribs.



Extras: Filter Print Post Top
Offlinefelixhigh
Scientist
Male User Gallery

Registered: 06/24/01
Posts: 7,557
Loc: Ly
Last seen: 1 month, 8 days
Re: Cactus tissue culture. [Re: neuro]
    #2056798 - 10/30/03 10:30 AM (20 years, 3 months ago)

una, thats it, you've crossed the barrier between the healthy and the insane, please do society a favour and go to intern yourself on a mental hospital.

you leave me without words. it's a little boring to repeat but:
AMAZING!!!


FH


Extras: Filter Print Post Top
OfflinestonErollEr1
The Psilocybinsolution
Registered: 05/23/01
Posts: 666 666 Posts!
Last seen: 13 years, 10 months
Re: Cactus tissue culture. [Re: felixhigh]
    #2059435 - 10/31/03 12:17 AM (20 years, 3 months ago)

Yep..this work is just AMAZING..and very very interesting to follow..
great work UNA..

peace..


Extras: Filter Print Post Top
InvisibleUna
controlleddemolition

Registered: 03/01/01
Posts: 970
Re: Cactus tissue culture. [Re: Una]
    #2102072 - 11/13/03 02:14 PM (20 years, 2 months ago)

I just received a beautiful variegated Tr. peruvianus and i couldn't resist setting up some new explants. We'll see what comes out, variegated, yellow or green shoots...

Here's the tip of the plant:



Surface sterilisation of the tip in household bleach on a magnetic stirrer plate. This is followed by three rinses with sterile water.



The tip is cut into sections.



And these are placed on the medium.



Once finished the jars are taped with breathable bandage tape. And yes, those are urine sample jars :laugh:



Here's some Lophophora jourdaniana



And some Lophphora williamsii seedlings



My pictures of the san pedro failed but they are developing new shoots on almost all areoles. Multiplication can be even faster than those 16,000 per year!


 


--------------------
www.911blogger.com


Extras: Filter Print Post Top
OfflineEkstaza
stranger than most
 User Gallery

Registered: 04/10/03
Posts: 4,324
Loc: Around the corner
Last seen: 9 months, 23 days
Re: Cactus tissue culture. [Re: Una]
    #2105249 - 11/14/03 07:23 AM (20 years, 2 months ago)

Stupendous. I love to see people with great intellect do great things. Una, you are a credit to the human race.
Wonderful
Amazing
Terrific
Outstanding


Yea, I guess that's enough


--------------------
YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]

Shop: Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Cacti tissue culture question llamabox 1,616 7 04/22/06 11:37 AM
by whatever123
* Tissue culture tek ?? the man 1,752 2 10/07/04 09:20 PM
by neuro
* Plant Tissue Culture mattymonkey 1,233 1 02/23/07 10:33 AM
by Schwip
* Tissue culture cloning jellyfish 4,463 7 08/25/06 06:24 PM
by karode13
* tissue culture cricket 1,230 4 02/19/05 01:01 PM
by Legoulash
* EZ and Cheap Cloning from Tissue Culture runnerup 3,387 6 12/24/04 01:00 PM
by pod3
* what is the procedure to make salvia tissue cultures? FGL 2,313 12 09/13/06 03:24 PM
by g0pher
* Tissue Culture / Microponics Baby_Hitler 3,270 11 05/19/03 08:32 PM
by neuro

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Mostly_Harmless, A.k.a
51,889 topic views. 2 members, 13 guests and 9 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.005 seconds on 13 queries.