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BigGreenMat
Stranger

Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Your v tek containers went awfully slow. Mine went full colonization from spores in 2 weeks but I used the recommended amount of brown rice. Not scientific I know. Just an observation. I am growing in low temps as well. I am really interested in your results. I am planning a similar test as soon as I have a good isolate instead of MS. As a city boy I want to see vtek do well because it is more space flexible.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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I have my bets on bulk
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Quote:
BigGreenMat said: Your v tek containers went awfully slow. Mine went full colonization from spores in 2 weeks but I used the recommended amount of brown rice. Not scientific I know. Just an observation. I am growing in low temps as well. I am really interested in your results. I am planning a similar test as soon as I have a good isolate instead of MS. As a city boy I want to see vtek do well because it is more space flexible.
Temps man. See how fast your cultures colonize in 65F then you will know why. It was fucking cold that week -13F. Still in identical temps the quarts still went faster. Temps are now solid at 70-72F.
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BigGreenMat
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Registered: 11/04/13
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Last seen: 3 years, 3 months
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I have been working at 65 as well (64-67 to be exact). Mine went quite fast as I said, but not really comparable. I am away right now and were 100% when I left. Hoping I come home after Christmas to some fruit to print and clone.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Quote:
BigGreenMat said: Mine went full colonization from spores in 2 weeks
Quote:
BigGreenMat said: I have been working at 65 as well (64-67 to be exact).

Sorry but assuming you were working with cubes I have a hard time believing this.
Unless. . .
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BigGreenMat said: I used the recommended amount of brown rice.
. . .you are referring to the "culture tek". In which case I have plates done from spores that germinated and achieved full colonization in a week and a half. Not really a valid comparison to what we are doing here
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 29 days
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Quote:
Pastywhyte said:
Quote:
BigGreenMat said: Mine went full colonization from spores in 2 weeks
Quote:
BigGreenMat said: I have been working at 65 as well (64-67 to be exact).

Sorry but assuming you were working with cubes I have a hard time believing this.
Unless. . .
Quote:
BigGreenMat said: I used the recommended amount of brown rice.
. . .you are referring to the "culture tek". In which case I have plates done from spores that germinated and achieved full colonization in a week and a half. Not really a valid comparison to what we are doing here 
I love seeing ppl post stuff like that. Hey if you're telling the truth good job dude and get a clone from it. If not... why don't you post your miracle cubes to some noobs that will believe you. I guarantee no one in this thread will believe w/o pics and even then idk.
Again tho I don't wanna come off as too much of dick so if it really did happen congrats and get a few clones goin from it.
Pasty keep up the good work im watching and GL!
Edited by magickspore (12/24/13 02:13 PM)
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BigGreenMat
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Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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Pasty I just went back to double check my calendar. I nocced my vtek invitros on December 4th. I used a ms APE syringe. I reached 100% on December 16th. This was with my 24 hour thermometer showing consistent temps of 64-67. It might have been lucky growth and that I used the amount violet specified in her tel. Also I am using brown rice. So lots of variables are different. I was very pleasantly surprised with my growth from ms at my temps.
Oh I used the glad quart container and filled it about a quarter full so about a half pint which is the recommended amount by violet for the containers you are using. So i also had a bit more initial gas because i wanted to get as clean a print as possible.
Edited by BigGreenMat (12/24/13 02:25 PM)
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Quote:
magickspore said:
I love seeing ppl post stuff like that. Hey if you're telling the truth good job dude and get a clone from it. If not... why don't you post your miracle cubes to some noobs that will believe you. I guarantee no one in this thread will believe w/o pics and even then idk.
a good innoc, done well and generously, will do it.
its beyond your frame of reference magick ,but not mine.
ill take him at his word.
i watch over the v tek crowd closely, i havent seen any distortions yet. we dont need to. wich is sorta our point.
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Edited by anne halonium (12/24/13 02:58 PM)
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BigGreenMat
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Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
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I never thought Anne would be defending me. I am not making any claims, just mentioning my very limited experience. I currently don't have the cultures or experience necessary to do the test that Pasty is, but I look forward to having that capability sometime soon in the future. I was not in any way criticizing Pasty either, just surprised how long the containers took.
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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it can vary depending. your results are plausable under some circumstances. im most known for growing in hot climates. but, over 35 yrs, i have grown in some COLD places. just part of the depth of experience, new age growers count on annie for.........
annie always defends the truth , justice , and the new age grow way, along with good bio safety and quality work! i really NEED a bright green cape with an "A" on it.
in the new age, ya gotta stand for more than BS......... but, dont mind me, back to the grow show.
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Edited by anne halonium (12/24/13 03:36 PM)
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Violet



Registered: 12/06/11
Posts: 4,205
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I believe BigGreenMat and agree. Mine are almost always colonized by 7 days. Usually 4-6 if 71-74°F. I'd expect them to be done before 14 days even in 66°F. But then again I inoc thoroughly, don't grow out multi-spore, and have no patience for unnecessarily slow culture but rather a pickiness for speed.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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anne halonium
jaguarette



Registered: 05/07/13
Posts: 1,908
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Re: V-Tek meets Bulk [Re: Violet]
#19321856 - 12/24/13 03:43 PM (10 years, 2 months ago) |
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^ and violet needs purple cape with a big "V" on it..........
to the new age!
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Edited by anne halonium (12/24/13 03:44 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: V-Tek meets Bulk [Re: Violet]
#19321951 - 12/24/13 04:03 PM (10 years, 2 months ago) |
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Quote:
Violet said: I'd expect them to be done before 14 days even in 66°F.
My quart jars were. Temps were lower than 66F 
Quote:
Violet said: But then again I inoc thoroughly, don't grow out multi-spore, and have no patience for unnecessarily slow culture but rather a pickiness for speed.
G2G with the bulk of the inoculate in the pp5, my culture is not ms, and it is also fairly quick for me in regular temps. My spawn run is nearly finished (if I had spawned when it was 100% it would be done and fruiting).
Regardless of the temp it is obvious that the reason the pp5 got so far behind was the need for more GE. I'm thinking the lid should have had the 1/4" turn as soon as the grains had recovered or very shortly after. This may have allowed it to keep pace with quarts.
Frankly I see a lot of people (not just in this thread) talk about quick colonization in cool temps. I have yet to see it in a real time grow log. I have run through about 50 cultures in the last year, and no matter how fast they did respectively in the summer months here, they all without exception slow right down when winter hits. I laugh inside when I see people in Colorado complain its cold, or people from Arizona complain its dry. When temps of -53F and RH is 1% are common in the winter here, then you can tell me you grow in inhospitable conditions. Shit the other day at work I had a guy fresh off the boat from Poland crying about how bad the cold was, he had never seen anything like it
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Violet



Registered: 12/06/11
Posts: 4,205
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Clearly they wouldn't go slower in plastics than they would in jars because of the temps. I'm just surprised that you may be experiencing GE stalls. It literally never happens to me. I stretch to recall it having happened to me even a few times back in the day... except for over-loaded containers. I load them up to half every time now. For whatever reasons, whether they're inoc'd with colonized rgs or agar, they almost always colonize in under 10 days, never get GE stalls, and are Never opened until time for fruiting.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: V-Tek meets Bulk [Re: Violet]
#19322125 - 12/24/13 04:36 PM (10 years, 2 months ago) |
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I guess it was the overloading that did it then. Next run at this will be with all agar inoculations for all spawn recepticles. I guess part of this particular crack at this is to see how well if fits my current practices. I always do wedge to masters then G2G as my primary method of expansion.
I do think that temps had a role to play as well. Just as your pp5 may need less of a sterilization time due to the thinner plastic providing less of an insulator, that same thing might have allowed the jars to retain their heat better.
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BigGreenMat
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Registered: 11/04/13
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Last seen: 3 years, 3 months
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Hmm is pp5 less insulating? I know polypropylene is more thermally insulating than glass... But given the differences in mass between the 2 containers I am not sure. Also no gas exchange in the pp5 should make it more resistant to exterior cold air.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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The whole reason for shorter sterilization times with the pp5 according to the v tek was the lower insulating value of the pp5 containers due to the thinner walls, most of the time spent with glass was due to the heat needing to penetrate the thick glass. Insulation values work both ways.
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BigGreenMat
Stranger

Registered: 11/04/13
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Last seen: 3 years, 3 months
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Ah yeah. Good point. Guess the mass is the bigger factor in this case.
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 29 days
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Quote:
anne halonium said:
Quote:
magickspore said:
I love seeing ppl post stuff like that. Hey if you're telling the truth good job dude and get a clone from it. If not... why don't you post your miracle cubes to some noobs that will believe you. I guarantee no one in this thread will believe w/o pics and even then idk.
a good innoc, done well and generously, will do it.
its beyond your frame of reference magick ,but not mine.
ill take him at his word.
i watch over the v tek crowd closely, i havent seen any distortions yet. we dont need to. wich is sorta our point.
Please do not take my words out of context like that I say what I mean and mean what I say... everytime and every word.
I believe this Again tho I don't wanna come off as too much of dick so if it really did happen congrats and get a few clones goin from it. was also in that post.
Also I only say that bec as pasty stated above I have yet to see very many grow logs where colonization occurred from spore to 100%. In 14 days or less. Usually its 14+ days.
I do not doubt it could happen I just have yet to experience it enough to consider it more than a fluke but as you stated Anne, my experiences are limited and that point of view will gladly change with some consistent proof.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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don't mind her and her standards your fine by the rest of us
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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