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Offlinemr.freeze
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Registered: 12/24/13
Posts: 13
Last seen: 9 years, 9 months
My mycelium syringes are frozen
    #19320596 - 12/24/13 11:11 AM (10 years, 1 month ago)

Bought some mycelium spores and when they arrived they were frozen.  They are liquid form in syringes.  How long they were frozen I don't know, I've been leaving them out in room temp.  I got them out of mail box 20 minutes ago, right now its hovering around 10 degress.  Are they still good?


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: My mycelium syringes are frozen [Re: mr.freeze]
    #19320598 - 12/24/13 11:12 AM (10 years, 1 month ago)

Probly not:frown:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineAero
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Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
Re: My mycelium syringes are frozen [Re: cronicr]
    #19320694 - 12/24/13 11:36 AM (10 years, 1 month ago)

shoot a few test jars, u might get lucky


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SPREAD THE SPORES



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OfflineNOTFALL3N
Epic

Registered: 11/18/13
Posts: 371
Last seen: 3 years, 10 months
Re: My mycelium syringes are frozen [Re: cronicr]
    #19320696 - 12/24/13 11:36 AM (10 years, 1 month ago)

D***  that sucks dude there proably bad! If it's from a sponser just let them know what happen should replace it. GL merry x-mad lol


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Offlinetwistedty
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Registered: 07/01/12
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Re: My mycelium syringes are frozen [Re: NOTFALL3N]
    #19320750 - 12/24/13 11:47 AM (10 years, 1 month ago)

betcha they are fine, use em


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Offlinekaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 9 years, 11 months
Re: My mycelium syringes are frozen [Re: twistedty]
    #19320921 - 12/24/13 12:20 PM (10 years, 1 month ago)

If its anything like viruses, every freeze thaw cycle will reduce the number of viable particles by about a log, but if there were a lot of spores there to begin with, you should still be able to grow them. The danger is if there were many freeze thaw events during shipping...


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What's a signature?


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: My mycelium syringes are frozen [Re: kaptanoblivious]
    #19320942 - 12/24/13 12:25 PM (10 years, 1 month ago)

I can say from experience they are most likely dead. The crystallization of the water in cell walls has destroyed your spores.


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineNOTFALL3N
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Registered: 11/18/13
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Re: My mycelium syringes are frozen [Re: maddchef]
    #19320959 - 12/24/13 12:29 PM (10 years, 1 month ago)

Let your vendor know the problem!!!!


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OfflinecronicrFacebook
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Re: My mycelium syringes are frozen [Re: maddchef]
    #19320962 - 12/24/13 12:29 PM (10 years, 1 month ago)

Yep i bet there fucked, never worked for me even with woodlo ers


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 9 years, 11 months
Re: My mycelium syringes are frozen [Re: maddchef]
    #19320967 - 12/24/13 12:31 PM (10 years, 1 month ago)

Ah well experience trumps speculation any day. There are cryoprotectants that can be added when freezing cell s to keep them more intact. I wonder if vendors could utilize something like this in the winter..


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What's a signature?


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OfflinecronicrFacebook
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Re: My mycelium syringes are frozen [Re: kaptanoblivious]
    #19321012 - 12/24/13 12:41 PM (10 years, 1 month ago)

Mine were frozen for god knows how long....fridge setting was to cold but it had to have been a month at least


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineBigGreenMat
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Registered: 11/04/13
Posts: 161
Last seen: 3 years, 3 months
Re: My mycelium syringes are frozen [Re: cronicr]
    #19321041 - 12/24/13 12:48 PM (10 years, 1 month ago)

My knowledge of this particular strain is limited. Cubes are tropical so the odds are against you.  Spores are generally much more hearty than mature fungus so just shoot a bunch into a culture and hope for the best. You might be surprised.

As for vendor cryoprotection. They should be able to put some glycerol in to make them freeze resistant.


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Offlinetwistedty
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Re: My mycelium syringes are frozen [Re: BigGreenMat]
    #19321119 - 12/24/13 01:06 PM (10 years, 1 month ago)

just shoot up some jars and let us know


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OfflinecronicrFacebook
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Re: My mycelium syringes are frozen [Re: twistedty]
    #19321130 - 12/24/13 01:08 PM (10 years, 1 month ago)

Your title is wrong...there spore syringes


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinetwistedty
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Registered: 07/01/12
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Re: My mycelium syringes are frozen [Re: cronicr]
    #19321153 - 12/24/13 01:14 PM (10 years, 1 month ago)

Quote:

cronicr said:
Your title is wrong...there spore syringes




:rofl:


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Offlinenn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
Re: My mycelium syringes are frozen [Re: twistedty]
    #19321180 - 12/24/13 01:20 PM (10 years, 1 month ago)

They are toasted... probably burst their cell walls from the expansion in the syringe due to the ice crystallization.. bummer man. Might as well try though! And email your vendor, let them know the situation.


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Offlinemr.freeze
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Registered: 12/24/13
Posts: 13
Last seen: 9 years, 9 months
Re: My mycelium syringes are frozen [Re: mr.freeze] * 1
    #19545910 - 02/09/14 10:20 PM (9 years, 11 months ago)

To everyone I went through with it and all four of them worked fine.


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Offlinenourished
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Registered: 05/17/13
Posts: 56
Last seen: 9 years, 2 months
Re: My mycelium syringes are frozen [Re: mr.freeze]
    #19546435 - 02/10/14 02:14 AM (9 years, 11 months ago)

How long could a syringe last frozen.?


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