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Offlinemotts
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Registered: 02/26/03
Posts: 31
Last seen: 10 years, 28 days
b+ colonization problems using Pf tek
    #19320353 - 12/24/13 09:56 AM (10 years, 1 month ago)

Ok so I'm on my 3rd grow attempt, still with not much luck. My 1st was with the PE strain, which didn't do too well on cakes. They produced a pathetically small amount, even tho I achieved a seemingly healthy mycelium growth on all the jars.
Round 2 was with a b+ syringe which was apparently damaged by heat during shipping and produced no mycelium at all. The seller confirmed that he had several problems with that particular batch and sent out replacements.
This brings us to attempt to 3, which is the replacement for the damaged b+ syringe. All 3 times I followed the Pf tek pretty much to a tee. Half pint wide mouth Mason jars, 2:1:1 ratio of verm to water to brf, steam Sterilized for 1.5 hrs, topped with a little dry verm, and used a syringe from a very legit and reputable source to inoculate about 1cc per each of 4 holes in top of jars.
So far it's been just approx 16-18 days, and I am experiencing some growth in one jar, minimal in another, and nothing at all in the rest. I have 9 jars total. They are stored at room temp in a dark closet, ranging from 70 - 73 degrees. The jar with the most growth has a quarter size spot on one side, and one the opposite side that's about 1/4 the size. The other with minimal growth has a spot slightly smaller than a pencil eraser head. Since I've noticed the growth (about 1 week ago) , it has shown only a very minimal change.  No signs of contam in any of the jars.  I will post pics soon.

I am concerned that something is wrong since most of my jars aren't doing anything, and the ones that are aren't doing much. I thought I'd try this strand bc I read it was an easier grow, but this far into the attempt I'm  starting  to worry! Am I doing something wrong that I'm not seeing? I would really appreciate any advice or pointers from you well seasoned pros out there. Thanks so much and Merry Christmas!!


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OfflineNOTFALL3N
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Registered: 11/18/13
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19320370 - 12/24/13 10:00 AM (10 years, 1 month ago)

Do u still have the tinfoil/tape on the holes? (Probably no the reason) sounds like ur doing everything right and they don't have to sit in the dark light is ok!


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Offlinemotts
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Registered: 02/26/03
Posts: 31
Last seen: 10 years, 28 days
Re: b+ colonization problems using Pf tek [Re: NOTFALL3N]
    #19321264 - 12/24/13 01:43 PM (10 years, 1 month ago)

Yes, I did take the foil off but the tape is still on. It's obviously punctured from the syringe tho, so I assumed it could be left on. Should I remove it you think? Also, is light Ok or do you recommend exposing it to light regularly to help with growth?


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OfflineNOTFALL3N
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Registered: 11/18/13
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19322270 - 12/24/13 05:22 PM (10 years, 1 month ago)

U can just take the tape off and light is not a problem. It will actually help and takeing the tape off will to!


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Offlinemotts
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Registered: 02/26/03
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Re: b+ colonization problems using Pf tek [Re: NOTFALL3N]
    #19322294 - 12/24/13 05:27 PM (10 years, 1 month ago)

Thanks for the insight! I will try that out. Thanks a bunch


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OfflinePussyFart
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19322305 - 12/24/13 05:30 PM (10 years, 1 month ago)

Quote:

motts said:
They are stored at room temp in a dark closet, ranging from 70 - 73 degrees.



Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


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OfflineNOTFALL3N
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Re: b+ colonization problems using Pf tek [Re: PussyFart]
    #19322610 - 12/24/13 07:05 PM (10 years, 1 month ago)

:whatshesaid:


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Offlinemotts
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Registered: 02/26/03
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Re: b+ colonization problems using Pf tek [Re: NOTFALL3N]
    #19323902 - 12/25/13 12:23 AM (10 years, 1 month ago)

Thanks for taking the time to respond in such detail!  I guess my dark closet may be the problem! I can fix that. Now here's my next question... Since its been so long since inoculation, could it be too late for the spores to recover and grow well?  Would they be able to survive approximately 2.5 weeks in the given conditions and start producing healthy mycelium once exposed to a circadian rhythm?


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Offlinenewera
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19323995 - 12/25/13 01:09 AM (10 years, 1 month ago)

I doubt no light causes no germination, but if your cake isn't contammed just wait it out. Remember to shake your syringe well before u inoculate too, spores need to bump eachother or find eachother to germinate, not sure if that's even a reason for the shake but both stand true


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Offlinemotts
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Re: b+ colonization problems using Pf tek [Re: newera]
    #19328623 - 12/26/13 12:24 PM (10 years, 1 month ago)

Yes I shook the syringe pretty good. There were a bunch of black chunks in it whichiI assume were spores. I wouldn't be so worried if there was at least some growth on all or even most of the jars. Not to be pessimistic, but how long should I wait to see some kind of action before I should start losing hope and consider starting over?

Pic 1: jar with a tiny little spot.
Pic 2 & 3: both sides of the jar really doing anything



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OfflinePussyFart
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19328733 - 12/26/13 12:51 PM (10 years, 1 month ago)

Spores can take 2+ weeks in some cases to germinate and show growth.

If there is nothing by day 21 I would start over.


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Offlinemotts
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Registered: 02/26/03
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Re: b+ colonization problems using Pf tek [Re: motts]
    #19341725 - 12/29/13 02:06 PM (10 years, 1 month ago)

Now I'm right around the 3 weeks mark with little to no changes. I'm baffled at the fact that there's no growth! Even the jars that had some growth seem to have stopped progressing. My 1st time around with the PE strain had phenomenal mycelium growth, and I only did one thing different... I used organic whole grain rice and ground it down myself.

Does anyone have any suggestions on the brf to use? I used a quality name  brand  brown rice flour for the past 2 batches that failed. What do most ppl usually use? Could it really be that the fresh ground home made brf made all the difference, or is it just coincidence??


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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Re: b+ colonization problems using Pf tek [Re: motts]
    #19341761 - 12/29/13 02:15 PM (10 years, 1 month ago)

I don't know what the problem is but a couple of things you could think about trying is next time do half organic four half non-organic and see if that makes a difference.  You could try a different spore syringe from a different vendor and use 2 syringes and see what happens there too if you can afford the extra $.  The next step from there might be agar, depending on how far you want to go.


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Offlinecrackacola
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Registered: 12/14/13
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Last seen: 8 years, 9 months
Re: b+ colonization problems using Pf tek [Re: invitro]
    #19344490 - 12/30/13 01:22 AM (10 years, 1 month ago)

You have mycelium... Ur substrate looks dark for BRF.  Not sure what that's about.  Are you leaving your stove on high setting while pressure cooking?  I'd say, if they're stalled, they probably need more GE.  You may have a contam in there, but it doesn't look like it.  Maybe your temp got too high?


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