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OfflinePNW UW
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Registered: 11/05/13
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Should i birth? 90% colonization at 4 weeks
    #19319680 - 12/24/13 04:23 AM (10 years, 1 month ago)

I believe my jars may be stalled, these are cubensis being kept in a TiT at 77-80 degrees. They are on an timed fan, supplying fresh air for 20 minutes every 2 hours.
Edit: meant 77-80 not 87-80  :blazed:
also the jars have open holes left from inoculation, i had micropore on until they got to about 50% colonization and i figured contams were less of a risk

worthy to note that out of this 8 jar batch, 2 got trich and one got cobweb inside the jar. (its really not a surprise, i spotted so many sterility problems that i have worked on with my second batch). those 3 jars were disposed of.

This is my first attempt at cultivation, so i know my main mistake is that my BRF/verm mix was lumpy and uneven (loaded with large spatula type spoon)
I believe my jars may be stalled,  that or the uncolonized areas i see at the top is just the dry verm layer that fell down.

Tried to get some good pictures here, do these look ready to birth? im not sure if these are 90% or 100%, because the top might be dry verm, or uncolonized substrate.

Thoughts would be greatly appreciated :smile:

Jar #1




Jar #2, might be contam since its stalled pretty badly.



Edited by PNW UW (12/25/13 01:57 AM)


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Offlinecrackacola
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Registered: 12/14/13
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Re: Should i birth? 90% colonization at 4 weeks [Re: PNW UW]
    #19319706 - 12/24/13 04:45 AM (10 years, 1 month ago)

Best to just wait it out until they start pinning in jar. Top two look like they'd be ok. What do you have for GE?  4 holes on top?  Any tape covering them?


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Edited by crackacola (12/24/13 02:14 PM)


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Offline1down5up
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Re: Should i birth? 90% colonization at 4 weeks [Re: crackacola]
    #19319718 - 12/24/13 04:51 AM (10 years, 1 month ago)

i would get the temps a little lower, and there's no need for the fan, they don't need that, they just need some holes on top for GE.  Do they have holes on the top?  4 weeks seems ok man, i would just wait it out.  They still have to consolidate yet, so give'm another weeks or so...

.02


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Offlinesuperkush89
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Re: Should i birth? 90% colonization at 4 weeks [Re: 1down5up]
    #19320007 - 12/24/13 07:57 AM (10 years, 1 month ago)

I would wait too. I also just started growing but I regret not leaving them in the jars for an extra week even tho the were at 100% cause my fruiting took an extra week to start anyway


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OfflineDetectiveLefty
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Re: Should i birth? 90% colonization at 4 weeks [Re: PNW UW]
    #19320113 - 12/24/13 08:40 AM (10 years, 1 month ago)

The top 2 jars look pretty close, the bottom one is not ready. Recommend waiting to birth until the cakes start to pin. This is assuming you're colonizing at regular light levels, and that you meant a temp range of 77-80 degrees. Otherwise, 87 is much too hot, and they won't pin in the dark.

By the way unless it's really cold in your house (under 65 degrees), you probably don't really need the TiT to incubate- you should be okay just setting them on a well-lit shelf. Definitely don't need the fan :smile:

Full colonization for me usually takes about 4 weeks at 75-80 degrees, but I've had it take longer if it's colder or when the cakes are colonizing unevenly, up to 6 weeks or longer. They do seem to slow down after 4 weeks probably because of high co2 levels, but they eventually colonize anyway.

Since the cakes shrink as they colonize, some of the dry verm often falls down- you can colonize the jars upside down to prevent this, but it really doesn't seem to matter since the cakes are resistant to contams at that point.

I like crackacola's idea of fruiting the 3rd cake in a separate FC- call it a quarantine FC maybe?


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OfflineNOTFALL3N
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Re: Should i birth? 90% colonization at 4 weeks [Re: DetectiveLefty]
    #19320356 - 12/24/13 09:57 AM (10 years, 1 month ago)

No wait growing shrooms takes patience just wait man be safe!


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Offlinecrackacola
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Re: Should i birth? 90% colonization at 4 weeks [Re: NOTFALL3N]
    #19321418 - 12/24/13 02:16 PM (10 years, 1 month ago)

Top two are 100% colonized, at least on the sides shown.  What you're seeing is his dry verm layer falling due to his substrate shrinking.  Just wait until you see pins if you're not sure how long they've been consolidating.  Or another week at least.  Answer questions about GE.


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InvisiblePirateSwazey
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Re: Should i birth? 90% colonization at 4 weeks [Re: crackacola]
    #19321594 - 12/24/13 02:49 PM (10 years, 1 month ago)

:whathesaid:

2nd jar lookin like the bacterial juju's goin on in there.


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Offlinecrackacola
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Re: Should i birth? 90% colonization at 4 weeks [Re: PirateSwazey]
    #19321674 - 12/24/13 03:05 PM (10 years, 1 month ago)

I think P-Sway meant the third jar.  I don't see any evidence of that with top two, just condensation, which is normal.  Definitely do not birth that 3rd jar anywhere near your other jars.


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OfflineOgreLokon
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Re: Should i birth? 90% colonization at 4 weeks [Re: DetectiveLefty]
    #19321917 - 12/24/13 03:57 PM (10 years, 1 month ago)

Quote:

DetectiveLefty said:
you can colonize the jars upside down




Ignore that part. Flipping jars just invites contams and invitro pinning. This is a game of patience.

Just ignore the jars for a week, and you'll be happier than if you look at them constantly.


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Offlineaomoriakuma
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Re: Should i birth? 90% colonization at 4 weeks [Re: OgreLokon]
    #19322224 - 12/24/13 05:08 PM (10 years, 1 month ago)

having dealt with more bacterial contams than id like to admit.. that third jar definitely looks like some bacterial contam is stalling mycelial growth. You can let it sit for a week and check on it again then, but if im right, i doubt anything will have changed.

Good luck!


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OfflinePNW UW
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Re: Should i birth? 90% colonization at 4 weeks [Re: PirateSwazey]
    #19324166 - 12/25/13 02:14 AM (10 years, 1 month ago)

Thanks for all the great resopnses guys :laugh:
ill keep this updated and post more pictures in a few days. the other 3 jars seem to be doing excelent, fully colonized, i now see that the verm on them is probably just the dry verm layer. since im new im going to wait until i see pinning. i can be patient now anyways, next quarter starts soon and wont have time to adventure :P.


Quote:

1down5up said:
i would get the temps a little lower, and there's no need for the fan, they don't need that, they just need some holes on top for GE.  Do they have holes on the top?  4 weeks seems ok man, i would just wait it out.  They still have to consolidate yet, so give'm another weeks or so...

.02



I had micropore for first 2 weeks, but at 50% colonization removed them for better GE. i think i might be doign overkill with keeping the holes open and using the fan, im guessing 5 jars doesnt need that extreme ammoutn of GE. XD


Quote:

DetectiveLefty said:
The top 2 jars look pretty close, the bottom one is not ready. Recommend waiting to birth until the cakes start to pin. This is assuming you're colonizing at regular light levels, and that you meant a temp range of 77-80 degrees. Otherwise, 87 is much too hot, and they won't pin in the dark.

By the way unless it's really cold in your house (under 65 degrees), you probably don't really need the TiT to incubate- you should be okay just setting them on a well-lit shelf. Definitely don't need the fan :smile:

Full colonization for me usually takes about 4 weeks at 75-80 degrees, but I've had it take longer if it's colder or when the cakes are colonizing unevenly, up to 6 weeks or longer. They do seem to slow down after 4 weeks probably because of high co2 levels, but they eventually colonize anyway.

Since the cakes shrink as they colonize, some of the dry verm often falls down- you can colonize the jars upside down to prevent this, but it really doesn't seem to matter since the cakes are resistant to contams at that point.

I like crackacola's idea of fruiting the 3rd cake in a separate FC- call it a quarantine FC maybe?



Forgot to mention i have a led strip on 12/12 with white tape over it (figured when it comes time to fruit, removing the tape will release a brighter spectrum that will trigger pinning)

I think as you mentioned it might be just that they are growing unevenly, i did a few things wrong. one was mixing the verm with the brf, and then adding water. now that i see the consistency of verm+water THEN brf, i see how soggy and lumpy these jars are ><. some even have crevices as if they were ice cream scooped. (my next jars  are much prettier XD)
ill see if i can whip up a fruting chamber with what i have lying around, is the bucket tek still respected?


Quote:

crackacola said:
Top two are 100% colonized, at least on the sides shown.  What you're seeing is his dry verm layer falling due to his substrate shrinking.  Just wait until you see pins if you're not sure how long they've been consolidating.  Or another week at least.  Answer questions about GE.



Awesome :laugh: ill just keep them in there until i see some change. looking at them now it makes a lot more sense to be the dry verm :laugh:


Quote:

crackacola said:
I think P-Sway meant the third jar.  I don't see any evidence of that with top two, just condensation, which is normal.  Definitely do not birth that 3rd jar anywhere near your other jars.



Quote:

aomoriakuma said:
having dealt with more bacterial contams than id like to admit.. that third jar definitely looks like some bacterial contam is stalling mycelial growth. You can let it sit for a week and check on it again then, but if im right, i doubt anything will have changed.

Good luck!



Quote:

PirateSwazey said:
:whathesaid:

2nd jar lookin like the bacterial juju's goin on in there.



Yesss, i couldnt get a clear picture of it, but the uncolonized areas in the #3rd picture look kind of white and 'misty'. and the mycelium around it lost its sharp ryzomorphic(sp) features, it stopped growing and now seems to be clouding up. Just admiting this now i think i just realized its definitely  contam XD. it(im just now realizing that its nearly impossible to catch any of the detail on these jars with a phone camera ><. even 8MP im taking 20 to find one good one.)


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OfflinePussyFart
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Re: Should i birth? 90% colonization at 4 weeks [Re: PNW UW]
    #19324183 - 12/25/13 02:23 AM (10 years, 1 month ago)

Quote:

PNW UW said:
Forgot to mention i have a led strip on 12/12 with white tape over it (figured when it comes time to fruit, removing the tape will release a brighter spectrum that will trigger pinning)



Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


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Offline1down5up
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Re: Should i birth? 90% colonization at 4 weeks [Re: PussyFart]
    #19324511 - 12/25/13 06:05 AM (10 years, 1 month ago)

:whathesaid:

and get rid of the fan...you won't need it for anything unless you're fruiting them in a mono.  You can use it for drying them as well.


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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