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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 7 years, 10 months
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Bacteria Contaminated tops of PF jars
#19317685 - 12/23/13 06:36 PM (10 years, 1 month ago) |
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Ok... So if ALL of my jars are contaminating with bacteria.... But always on the top... What could be the reason for this? I take the jars out while their still hot and leave the tin foil on until inoculation. Is that bad?
I thought contaminated inoculant... But the mycelia that I inject colonized the bottom 1/2 to 3/4 of the jars.... The bacteria is always at the top
I also have control PF jars that have basically grain lids... With silicone ports and a SFD. They seem to be doing fine?!?!
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TheMustardTiger


Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
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Re: Bacteria Contaminated tops of PF jars [Re: trip352]
#19317780 - 12/23/13 06:53 PM (10 years, 1 month ago) |
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Quote:
trip352 said: Ok... So if ALL of my jars are contaminating with bacteria.... But always on the top... What could be the reason for this? I take the jars out while their still hot and leave the tin foil on until inoculation. Is that bad?
This is a no-no. Leave them in your pressure cooker overnight or at the very least let them come down to room temp.
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NOTFALL3N
Epic

Registered: 11/18/13
Posts: 371
Last seen: 3 years, 10 months
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Do you use a dry Verm layer on top and don't tip it over while it colonizing
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
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Re: Bacteria Contaminated tops of PF jars [Re: NOTFALL3N]
#19317985 - 12/23/13 07:32 PM (10 years, 1 month ago) |
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bacterial contams can mean too wet
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Bacteria Contaminated tops of PF jars [Re: twistedty]
#19318020 - 12/23/13 07:39 PM (10 years, 1 month ago) |
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Your going wrong somewhere...IME cakes dont get bacteria comtams that often, there too dry. Usually when it does happen its from the syringe/too much juice/ not properly sterilized.make sure your starti g the time when the water begins to boil
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 7 years, 10 months
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Re: Bacteria Contaminated tops of PF jars [Re: cronicr]
#19321331 - 12/24/13 01:58 PM (10 years, 1 month ago) |
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I PC for 60min timer starting when the cooker goes above atmospheric
Also I do use a dry vermiculite barrier but when I checked it, it seemed wet some how...
Also I built a 4x2 flow hood but haven't fully confirmed it works yet :/ still need to do agar
Am I over cooking them? I know people boil in an un covered pot so I don't see that as the issue
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Camtaro420
Birdman



Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 5 years, 5 months
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Quote:
MustardTiger66 said:
Quote:
trip352 said: Ok... So if ALL of my jars are contaminating with bacteria.... But always on the top... What could be the reason for this? I take the jars out while their still hot and leave the tin foil on until inoculation. Is that bad?
This is a no-no. Leave them in your pressure cooker overnight or at the very least let them come down to room temp.
You need to leave them in the PC till around the hour you're going to inoc. If you're bringing out your PC'd jars while they are hot and now exposed you're just making an open invitation for bacterial contaminates to start producing in the heat and moisture sitting under the foil and lid where you're experiencing the issue.
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 7 years, 10 months
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Re: Bacteria Contaminated tops of PF jars [Re: Camtaro420]
#19328943 - 12/26/13 01:42 PM (10 years, 1 month ago) |
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I see... So should I take off the foil right away? Let them cool in front of the flow hood?
How then do people just boil PF jars?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Bacteria Contaminated tops of PF jars [Re: trip352]
#19328957 - 12/26/13 01:46 PM (10 years, 1 month ago) |
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just take the foil off and put them on a shelf or in your sab or anywhere till you inoculate just don't toss them around too much and disturb your verm barrier
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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trip352
the not so new guy



Registered: 03/23/11
Posts: 418
Last seen: 7 years, 10 months
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Re: Bacteria Contaminated tops of PF jars [Re: cronicr]
#19332975 - 12/27/13 01:14 PM (10 years, 1 month ago) |
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Ok thanks I'll try that.... I need a SAB still I just went ahead and built a flow hood lol
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Bacteria Contaminated tops of PF jars [Re: trip352]
#19332979 - 12/27/13 01:15 PM (10 years, 1 month ago) |
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awesome shit!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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