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OfflineAdustus
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Trying to figure out sub mix for bulk spawn
    #19314704 - 12/23/13 04:41 AM (10 years, 2 months ago)

So I have some grain that will be ready in awhile. Im trying to get well equipped on some info about my substrate mix.

I have everything from pine shavings to straw to coffee and newspaper and even cardboard and organic green tea.

Im trying to get a good idea of what Id wanna do here for a bulk spawn from rye berry jars.


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OfflinePussyFart
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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314707 - 12/23/13 04:43 AM (10 years, 2 months ago)

For what species?

Cubes like coir and verm, or manure and verm, or straw, or a mixture of them all.


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: PussyFart]
    #19314716 - 12/23/13 04:46 AM (10 years, 2 months ago)

Its a texas Cube.

Im also looking into what to use for my reishi as it will be going in a tub around the same time.

Im still waiting for spores to germinate on agar. I cant believe how long that shit takes.


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314730 - 12/23/13 04:53 AM (10 years, 2 months ago)

Ok so of my possible mixes of what I have, what would you recommend I use for a mix? And how would you sterilize it?

Im going to look into some coir. But thats only a hope.


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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314735 - 12/23/13 04:55 AM (10 years, 2 months ago)

Quote:

Adustus said:
Ok so of my possible mixes of what I have, what would you recommend I use for a mix? And how would you sterilize it?

Im going to look into some coir. But thats only a hope.




pasteurize not sterilize.  coir/verm is easiest and proven, and easy to find.

http://www.shroomery.org/forums/showflat.php/Number/17897163#17897163

also

www.mushroomvideos.com


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: twistedty]
    #19314758 - 12/23/13 05:06 AM (10 years, 2 months ago)

I understand that. But I have to make do with what I have. Unless some fat bastard in a suit is gonna bring it on wednesday:laugh2:

Dude the holidays have broken my finances so hard this year. Alot of this stuff I obtained through various trading with farmers and local businesses. And good ole recycling.:thumbup:

I can prolly get some horse or cow poo if I go searching for it.


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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314763 - 12/23/13 05:09 AM (10 years, 2 months ago)

Quote:

Adustus said:
I understand that. But I have to make do with what I have. Unless some fat bastard in a suit is gonna bring it on wednesday:laugh2:

Dude the holidays have broken my finances so hard this year. Alot of this stuff I obtained through various trading with farmers and local businesses. And good ole recycling.:thumbup:

I can prolly get some horse or cow poo if I go searching for it.





As with all hobbies it takes money :shrug: you should be able to get coir and verm for around 10 bucks


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Re: Trying to figure out sub mix for bulk spawn [Re: twistedty]
    #19314805 - 12/23/13 05:31 AM (10 years, 2 months ago)

Sounds like the eh fuck it method again. Seems to work out well. Ill get some cow poo and mix it with straw and coffee and well see where it goes.

Maybe mix some of the wood shavings in there just for the fuck of it. If it grows it grows. If not. Back to the drawing board and the books.


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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314815 - 12/23/13 05:36 AM (10 years, 2 months ago)

Pasteurized cardboard for reishi, sterilized shredded straw + coffee grounds for cubensis (in mycobags if you have them, jars if you don't).


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314828 - 12/23/13 05:45 AM (10 years, 2 months ago)

Quote:

Tangich said:
Pasteurized cardboard for reishi, sterilized shredded straw + coffee grounds for cubensis (in mycobags if you have them, jars if you don't).




Nice thats the answer I was looking for.

Are these pine shavings a loss then? Or will reishi eat them up?


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InvisibleTangich

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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314834 - 12/23/13 05:49 AM (10 years, 2 months ago)

I wouldn't use pine shavings for anything. Some species of softwood are okay for certain mushrooms, but others contain oils and other compounds that are inhibitive for most mushroom species. So unless you know the exact species of tree those came from, it's best to avoid them.


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314839 - 12/23/13 05:49 AM (10 years, 2 months ago)

Quote:

Tangich said:
Pasteurized cardboard for reishi, sterilized shredded straw + coffee grounds for cubensis (in mycobags if you have them, jars if you don't).




And how would you go about sterilizing the straw and coffee for a bulk spawn in a tub?


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314841 - 12/23/13 05:50 AM (10 years, 2 months ago)

Quote:

Tangich said:
I wouldn't use pine shavings for anything. Some species of softwood are okay for certain mushrooms, but others contain oils and other compounds that are inhibitive for most mushroom species. So unless you know the exact species of tree those came from, it's best to avoid them.




Guess that means this giant block of pine shavings is a loss. Fuck man.


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InvisibleTangich

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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314863 - 12/23/13 06:02 AM (10 years, 2 months ago)

Quote:

Adustus said:
And how would you go about sterilizing the straw and coffee for a bulk spawn in a tub?



In separate spawn bags, let them colonize, then partially crumble and fill the tub, and give them a few days to stick back together.
Straw can be easily pasteurized as well, which would probably be more convenient if you're working with large amounts. But personally, I would never trust coffee that is just pasteurized. You could separately colonize sterilized coffee, then mix it with pasteurized straw alongside the grain spawn.


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314866 - 12/23/13 06:05 AM (10 years, 2 months ago)

Quote:

Tangich said:
Quote:

Adustus said:
And how would you go about sterilizing the straw and coffee for a bulk spawn in a tub?



In separate spawn bags, let them colonize, then partially crumble and fill the tub, and give them a few days to stick back together.
Straw can be easily pasteurized as well, which would probably be more convenient if you're working with large amounts. But personally, I would never trust coffee that is just pasteurized. You could separately colonize sterilized coffee, then mix it with pasteurized straw alongside the grain spawn.




Well all I have is a PC. So do I PC jars of straw and dump it in a bag or something for the tub? Ive seen some people use bleach for cardboard and paper. Would that work with straw?

Ive never sterilized or pasteurized bulk substrates so some info would be very much appreciated. Id hate my only success to contam to hell in a bin from my lack of understanding .


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OfflineStromriderM
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314869 - 12/23/13 06:07 AM (10 years, 2 months ago)

I'd stay away from coffee in your bulk substrate and like ty said pasteurize don't sterilize. If you're just using coir and verm you can get by with sterilization

A brick of coir at petco is $3
A bag of stagreen verm at Lowes is $3


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InvisibleTangich

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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19314890 - 12/23/13 06:15 AM (10 years, 2 months ago)

Quote:

Adustus said:

Well all I have is a PC. So do I PC jars of straw and dump it in a bag or something for the tub? Ive seen some people use bleach for cardboard and paper. Would that work with straw?



Shred the straw in 1-2 inch pieces (mix it with coffee if you want), stuff the jars, sterilize them in the PC, then inoculate them with colonized grain spawn like you'd do a grain to grain transfer. Seal the jars and let them colonize completely, then empty them into a fruiting chamber.
Oh yeah, I forgot, you have to hydrate the straw for 24 hours before putting it in jars.
Sterilization is always best, but only if you can keep the substrate sealed completely until full colonization. So it obviously isn't practical for large quantities like monotubs etc.
Coir and verm are great water reservoirs, very useful as additives, but far from necessary.


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314904 - 12/23/13 06:20 AM (10 years, 2 months ago)

Quote:

Tangich said:
Quote:

Adustus said:

Well all I have is a PC. So do I PC jars of straw and dump it in a bag or something for the tub? Ive seen some people use bleach for cardboard and paper. Would that work with straw?



Shred the straw in 1-2 inch pieces (mix it with coffee if you want), stuff the jars, sterilize them in the PC, then inoculate them with colonized grain spawn like you'd do a grain to grain transfer. Seal the jars and let them colonize completely, then empty them into a fruiting chamber.
Oh yeah, I forgot, you have to hydrate the straw for 24 hours before putting it in jars.
Sterilization is always best, but only if you can keep the substrate sealed completely until full colonization. So it obviously isn't practical for large quantities like monotubs etc.
Coir and verm are great water reservoirs, very useful as additives, but far from necessary.




Good fortune left and right. Now I have my goody bag from spore works paid for. Got my petris paid for. Along with some verm and coir. I guess it all works out in the end.


Edited by Adustus (12/23/13 06:27 AM)


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OfflineAdustus
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Re: Trying to figure out sub mix for bulk spawn [Re: Tangich]
    #19314924 - 12/23/13 06:28 AM (10 years, 2 months ago)

Quote:

Tangich said:
Quote:

Adustus said:

Well all I have is a PC. So do I PC jars of straw and dump it in a bag or something for the tub? Ive seen some people use bleach for cardboard and paper. Would that work with straw?



Shred the straw in 1-2 inch pieces (mix it with coffee if you want), stuff the jars, sterilize them in the PC, then inoculate them with colonized grain spawn like you'd do a grain to grain transfer. Seal the jars and let them colonize completely, then empty them into a fruiting chamber.
Oh yeah, I forgot, you have to hydrate the straw for 24 hours before putting it in jars.
Sterilization is always best, but only if you can keep the substrate sealed completely until full colonization. So it obviously isn't practical for large quantities like monotubs etc.
Coir and verm are great water reservoirs, very useful as additives, but far from necessary.




This is some great info. Ill give this a shot once the grain is ready. Cant wait to do agar on petris. And Im going to find a way to make pine shavings less useless.


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Re: Trying to figure out sub mix for bulk spawn [Re: Adustus]
    #19315316 - 12/23/13 09:05 AM (10 years, 2 months ago)

I would look into some pasturization teks, much more forgiving and there  are tons of possibilities for subs. Ive used pine but it needs to be aged, ive grown on grass clippings and black berry bushes with a good pasturizing


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