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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
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Re: inoculating coir without spawn [Re: J. Jack Flash]
    #19307036 - 12/21/13 09:13 AM (10 years, 2 months ago)

Steam them for a strait eight hours or wait for your pc


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinegman7104

Registered: 09/11/11
Posts: 820
Last seen: 7 years, 3 months
Re: inoculating coir without spawn [Re: J. Jack Flash]
    #19309073 - 12/21/13 06:43 PM (10 years, 2 months ago)

Quote:

J. Jack Flash said:
as one who loves to deep fry stuff, in fact will be frying a ton tomorrow, can't say i'd ever consider it.  seems like it could work, but what a pain in the ass.  it would certainly throw off the timing, and i bet the jar would be buoyant, meaning you have to hold it under the entire time.  then when it's done, you gotta clean the oil off the jar(s) without getting the filter wet.  try that just one time and see how you like it.

or just wait for the pressure cooker.  =P

edit: yeah wait it positively will not work.  while you're waiting with the jar in the oil, all the water in the grain or whatever is gonna steam out the filter and your jar will dry out.  it may be sterile, but also barren.



Thats a good point you made about the water. thats exactly why it won't work. The jar wouldn't be bouyant due to the glass but it will dry out. I guess ima have to wait 2 weeks for my PC -.-

I was just hoping to find a possible solution. Theres no risk since I have tons of spores, if I start it now and throw it out by the time my PC returns it would be no different than not trying at all. Im just trying to find the most "likely to succeed" method.


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OfflinecronicrFacebook
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Re: inoculating coir without spawn [Re: cronicr]
    #19309371 - 12/21/13 08:10 PM (10 years, 2 months ago)

Quote:

gman7104 said:
I was just hoping to find a possible solution. Theres no risk since I have tons of spores, if I start it now and throw it out by the time my PC returns it would be no different than not trying at all. Im just trying to find the most "likely to succeed" method.




Quote:

cronicr said:
Steam them for a strait eight hours or wait for your pc



............


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinegman7104

Registered: 09/11/11
Posts: 820
Last seen: 7 years, 3 months
Re: inoculating coir without spawn [Re: cronicr]
    #19309448 - 12/21/13 08:30 PM (10 years, 2 months ago)

oh I never saw that. I don't think extending the time would matter, the problem is the temperature isn't hot enough.

Im going to try steaming the jars everyday for 4 days, allowing any contams to germinate and therefore become vulnerable to be killed at lower temperatures.

I don't see this working too well until I get my PC back though. Before my PC i would steam half pint BRF cakes. Every one of them grew mushrooms but they all ended up with bacteria or yeast or mold, which means it wouldn't work for spawn.

Guess im just gona have to wait. It just sucks because for the last 2 weeks I had EVERYTHING except the fucking needles for the syringes. Got that back now I lost my PC :mad2:


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OfflinecronicrFacebook
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Re: inoculating coir without spawn [Re: gman7104]
    #19309455 - 12/21/13 08:33 PM (10 years, 2 months ago)

fractional sterilization sux, steaming for eight hours strait works fine

steamed for a strait 8
http://www.shroomery.org/forums/showflat.php/Number/19296230
theres my adventure into steaming grain jars:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinecronicrFacebook
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Re: inoculating coir without spawn [Re: cronicr]
    #19309474 - 12/21/13 08:40 PM (10 years, 2 months ago)

Quote:

RogerRabbit said:
Quote:

cubensischool said:
Ive heard that you can succesfully steam sterilize whole grain(specifically WBS). The catch is that takes a hell of a lot longer. Like for 1/2 pint jars 2hrs a day for 3 days longer. This gives the bacteria specific to this substrate adequate time to become vulnerable enough to die



That method is outdated and prone to failure.  You can prepare and jar the grains as shown in Let's Grow Mushrooms and load into quart/liter jars.  Steam sterilize for 8 hours in one setting.  Keep a second pot of water boiling to use when adding water, because you'll need to do it every half hour or so.
RR





http://www.shroomery.org/forums/showflat.php/Number/15753434#15753434


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinegman7104

Registered: 09/11/11
Posts: 820
Last seen: 7 years, 3 months
Re: inoculating coir without spawn [Re: cronicr]
    #19309493 - 12/21/13 08:49 PM (10 years, 2 months ago)

Quote:

cronicr said:
Quote:

RogerRabbit said:
Quote:

cubensischool said:
Ive heard that you can succesfully steam sterilize whole grain(specifically WBS). The catch is that takes a hell of a lot longer. Like for 1/2 pint jars 2hrs a day for 3 days longer. This gives the bacteria specific to this substrate adequate time to become vulnerable enough to die



That method is outdated and prone to failure.  You can prepare and jar the grains as shown in Let's Grow Mushrooms and load into quart/liter jars.  Steam sterilize for 8 hours in one setting.  Keep a second pot of water boiling to use when adding water, because you'll need to do it every half hour or so.
RR





http://www.shroomery.org/forums/showflat.php/Number/15753434#15753434



Thanks for that reference. Il try the extended sterilization, and maybe use less water in my recipie to reduce the chance of  a bacteria infestation.


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OfflinecronicrFacebook
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Re: inoculating coir without spawn [Re: gman7104]
    #19309498 - 12/21/13 08:50 PM (10 years, 2 months ago)

no just prep your grains the way your suppose to, it will be fine:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinePocketRevolution
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Registered: 12/22/09
Posts: 270
Last seen: 1 month, 17 days
Re: inoculating coir without spawn [Re: RogerRabbit]
    #19314366 - 12/23/13 12:59 AM (10 years, 2 months ago)

Quote:

RogerRabbit said:
The answer is no.

The reason coir is so 'contamination resistant' is because for still-unknown reasons, fungi spores do not like to germinate on it.  Trich won't germinate on coir, and mushroom spores won't germinate on it either.
RR




I liked the part where you said "do not like to" better than where you said "won't".  They will, if you really make them.  For instance, if you were to take a jar of sterilized coir, and suspend a sporulating cap on a piece of hardware cloth in the mouth of the jar, mycelium would form in the coir.  It would be thready.  It would never form pins.  But it would grow.


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