|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
|
Genetics the cause of slow colonization of coir?
#19309145 - 12/21/13 07:12 PM (10 years, 1 month ago) |
|
|
So I have always had very similar results from spores that come from the same parent mushroom. Not identical, I know ms syringes produce unreliable and inconsistent results, but I've always got similar results. By all means not identical, I'm in no way refuting the common knowledge on "strain" and whatnot, I'm just saying that spores from the SAME syringe have always colonized a jar in roughly the same time. Certainly not to the day, but within a few days and noticeably different from other syringes. Alright, hopefully I won't get flamed for strain talk, now to the question. This particular syringe of spores has always colonized grain and hpoo fairly slow, and this has been tested several times against other, unrelated genetics. Please let it be known that I know what I'm talking about, I just see consistent-ish results from ms of the same syringe. Here's the problem, I tried using those same spores on a batch of coir, and it just refuses to colonize it at any reasonable rate. Could they be lacking a certain enzyme that breaks down and metabolizes coir? I mean, I don't pretend to know what I'm talking about with mushroom genetics, but if the parent mushroom lacked the enzyme then, if it was a dominant gene, all of the spores it produced would also lack that enzyme. Is it remotely possible that this could be the source of my problems? I have tried to get it to colonize coir in several small setups with different grain jars and there have been no funny smells so I really don't think contamination is an issue. Please someone help me out, I'm puzzled and don't really know what to do. Thanks in advanced.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Sgt. Pepper]
#19309203 - 12/21/13 07:29 PM (10 years, 1 month ago) |
|
|
how are you preparing your coir? are you talking about syringes you did yourself?
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 18 days
|
Re: Genetics the cause of slow colonization of coir? [Re: cronicr]
#19309215 - 12/21/13 07:34 PM (10 years, 1 month ago) |
|
|
You are shooting multispore into coir? Or you spawned it with grain?
|
Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
|
Re: Genetics the cause of slow colonization of coir? [Re: Amanita virosa]
#19309224 - 12/21/13 07:36 PM (10 years, 1 month ago) |
|
|
Quote:
Amanita virosa said: You are shooting multispore into coir? Or you spawned it with grain?
HAHAH, not gonna read the OP's text. Way too much text.
But to AV's question... If he read it and can't understand it.. I hope to Christ you did not inject the Coir with a needle. LMAO@. Good luck Bro. <-- kickin in
-------------------- "wise words here"
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Stool_Sample]
#19309377 - 12/21/13 08:12 PM (10 years, 1 month ago) |
|
|
Quote:
Stool_Sample said:
Quote:
Amanita virosa said: You are shooting multispore into coir? Or you spawned it with grain?
HAHAH, not gonna read the OP's text. Way too much text.
But to AV's question... If he read it and can't understand it.. I hope to Christ you did not inject the Coir with a needle. LMAO@. Good luck Bro. <-- kickin in
grade school was probly a bitch for you huh
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 18 days
|
Re: Genetics the cause of slow colonization of coir? [Re: cronicr]
#19309422 - 12/21/13 08:25 PM (10 years, 1 month ago) |
|
|
Quote:
cronicr said:
Quote:
Stool_Sample said:
Quote:
Amanita virosa said: You are shooting multispore into coir? Or you spawned it with grain?
HAHAH, not gonna read the OP's text. Way too much text.
But to AV's question... If he read it and can't understand it.. I hope to Christ you did not inject the Coir with a needle. LMAO@. Good luck Bro. <-- kickin in
grade school was probly a bitch for you huh
yea, lots of words!
|
Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Amanita virosa]
#19309534 - 12/21/13 09:01 PM (10 years, 1 month ago) |
|
|
Yeah, sorry about the wordiness, saying strain on here just causes a flame feat usually so I wanted to be thorough. But no, I spawned with wbs, and I had a fairly high spawn ratio.
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Sgt. Pepper]
#19309554 - 12/21/13 09:10 PM (10 years, 1 month ago) |
|
|
if they colonized grains they should colonize coir unless something is wrong beyond there ability
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
|
Re: Genetics the cause of slow colonization of coir? [Re: Sgt. Pepper]
#19309785 - 12/21/13 10:34 PM (10 years, 1 month ago) |
|
|
Quote:
Sgt. Pepper said: Yeah, sorry about the wordiness, saying strain on here just causes a flame feat usually so I wanted to be thorough.
So stop calling them strains if you know what you are talking about.....they are called varieties. lol
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
|
Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
|
Re: Genetics the cause of slow colonization of coir? [Re: PussyFart]
#19310794 - 12/22/13 06:59 AM (10 years, 1 month ago) |
|
|
Lol. It's just bizarre that all the spores from that syringe, after being spawned to wbs, refuse to colonize coir. I prepared it the same way as I always have and I'm 90% sure it isn't contaminated.
|
Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 6 months, 18 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Sgt. Pepper]
#19310830 - 12/22/13 07:13 AM (10 years, 1 month ago) |
|
|
If you shook up the wbs and spawned it to coir and it refuses colonize, then either your wbs spawn or the coir is contaminated with bacteria. My bet in on the wbs If you have other jars from that batch shake them and I bet they will fail to recolonize. Try it!
|
Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
|
Re: Genetics the cause of slow colonization of coir? [Re: Amanita virosa]
#19311249 - 12/22/13 10:02 AM (10 years, 1 month ago) |
|
|
Quote:
Amanita virosa said: If you shook up the wbs and spawned it to coir and it refuses colonize, then either your wbs spawn or the coir is contaminated with bacteria. My bet in on the wbs If you have other jars from that batch shake them and I bet they will fail to recolonize. Try it!
That's definitely possible, it's just weird cause it doesn't smell at all. And the grains do recover after shaking, they have little rhizo's reaching out from all the grains, just they don't want anything to do with the coir!
|
|