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InvisibleStuey1
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Registered: 11/28/13
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G2g transfer
    #19308087 - 12/21/13 01:35 PM (10 years, 1 month ago)

How many times can you continue to do g2g transfer? Can I just keep doing over and over countless times?


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OfflineAmanita virosa
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Re: G2g transfer [Re: Stuey1]
    #19308109 - 12/21/13 01:38 PM (10 years, 1 month ago)

How lucky do you feel?  I do not recommend past g3. Beyond that is a crap shoot. Now all these folks will come behind me and say how they have gone g4 g5....they got lucky.


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Offlinekaptanoblivious
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Re: G2g transfer [Re: Stuey1]
    #19308113 - 12/21/13 01:39 PM (10 years, 1 month ago)

I have seen this come up before... other people recommend about 4 consecutive transfers is where you should stop and go back to an earlier point, or start a new run. It was said that the cultures can start to grow slower/get weaker, but this is not my firsthand experience.


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InvisibleStuey1
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Re: G2g transfer [Re: kaptanoblivious]
    #19308136 - 12/21/13 01:48 PM (10 years, 1 month ago)

So is a g3 the 3rd generation of jars. Meaning 2 transfers have been made off the original master jar?


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OfflinePussyFart
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Re: G2g transfer [Re: Stuey1] * 1
    #19308152 - 12/21/13 01:52 PM (10 years, 1 month ago)

Quote:

Amanita virosa said:
How lucky do you feel?  I do not recommend past g3. Beyond that is a crap shoot. Now all these folks will come behind me and say how they have gone g4 g5....they got lucky.



It's got nothing to do with luck.....it's got to do with the amount of cell divisions that have taken place....and it can go past 3 transfers with great success.

Quote:

RogerRabbit said:
You can go much more than that.  On our shiitake farm, I usually do 5 grain to grain transfers, and my spawn bags hold nearly 10 quarts each.  This means we turn a single petri dish culture into at least 100,000 quarts of rye.  Beyond simple cell division, chronological time is very important.  If you stretch out the G2G transfers over a period of months, the mycelium will lose vigor the same as if it had been expanded.  This is why we refrigerate cultures.
RR



http://www.shroomery.org/forums/showflat.php/Number/16986910#16986910

Just sayin......


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OfflineAmanita virosa
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Re: G2g transfer [Re: PussyFart]
    #19308168 - 12/21/13 01:59 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

Amanita virosa said:
How lucky do you feel?  I do not recommend past g3. Beyond that is a crap shoot. Now all these folks will come behind me and say how they have gone g4 g5....they got lucky.



It's got nothing to do with luck.....it's got to do with the amount of cell divisions that have taken place....and it can go past 3 transfers with great success.

Quote:

RogerRabbit said:
You can go much more than that.  On our shiitake farm, I usually do 5 grain to grain transfers, and my spawn bags hold nearly 10 quarts each.  This means we turn a single petri dish culture into at least 100,000 quarts of rye.  Beyond simple cell division, chronological time is very important.  If you stretch out the G2G transfers over a period of months, the mycelium will lose vigor the same as if it had been expanded.  This is why we refrigerate cultures.
RR



http://www.shroomery.org/forums/showflat.php/Number/16986910#16986910

Just sayin......




The luck I refer to has to do with avoiding contamination not senescence. Senescence wise you could easily go farther with a fresh culture. But contamination wise it becomes an all or nothing proposition. A tiny unnoticeable bit of trich and you lose it all. Nota hacker is a gambler. Livin on the edge.  I guess that why he got all those ugly green guns. :lmafo:


Edited by Amanita virosa (12/21/13 02:09 PM)


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OfflinePussyFart
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Re: G2g transfer [Re: Amanita virosa]
    #19308271 - 12/21/13 02:30 PM (10 years, 1 month ago)

You call them ugly, I call them a necessity......

To each his own.....lol.

EDIT: And none of the guns are green, just the grips.

I could make them hot pink......


Edited by PussyFart (12/21/13 02:31 PM)


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OfflineAmanita virosa
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Re: G2g transfer [Re: PussyFart]
    #19308298 - 12/21/13 02:38 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
You call them ugly, I call them a necessity......

To each his own.....lol.

EDIT: And none of the guns are green, just the grips.

I could make them hot pink......






Welcome to north cakkalaky! 


Edited by Amanita virosa (12/21/13 02:49 PM)


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Offlinevikingsc
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Re: G2g transfer [Re: Amanita virosa]
    #19308356 - 12/21/13 03:00 PM (10 years, 1 month ago)

Is that a .50 cal top left?

In short, yes there is a limit. Many say g3 others go as far as g5 with success. :mushroom2: G2G's are easy with a flowhood. A little more difficult in a SAB.


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OfflinePussyFart
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Re: G2g transfer [Re: vikingsc]
    #19308386 - 12/21/13 03:08 PM (10 years, 1 month ago)

Yea, thats a Smith n Wesson 500 Magnum.....

I have an AK as well.......Maadi



Edited by PussyFart (12/21/13 03:08 PM)


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OfflineStromriderM
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Re: G2g transfer [Re: vikingsc]
    #19308395 - 12/21/13 03:10 PM (10 years, 1 month ago)

Now boys... :lmafo:


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Offlinevikingsc
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Re: G2g transfer [Re: PussyFart]
    #19308399 - 12/21/13 03:11 PM (10 years, 1 month ago)

Sick. I wish we were allowed to have Ak's and Ar15's here up north of the 49 :laugh:


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Offlinevikingsc
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Re: G2g transfer [Re: vikingsc]
    #19308407 - 12/21/13 03:13 PM (10 years, 1 month ago)

An old school looking stock on that Ak. Is that the one it came with?


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OfflineAmanita virosa
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Re: G2g transfer [Re: PussyFart]
    #19308416 - 12/21/13 03:16 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Yea, thats a Smith n Wesson 500 Magnum.....

I have an AK as well.......Maadi





Actually a .460 slightly smaller bore but Highest velocity of any handgun on earth.  Great for bear and boar. And really sorry to op for this awful high jack.


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OfflinePussyFart
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Re: G2g transfer [Re: Amanita virosa]
    #19308450 - 12/21/13 03:29 PM (10 years, 1 month ago)

Quote:

Amanita virosa said:
Quote:

Notahacker420 said:
Yea, thats a Smith n Wesson 500 Magnum.....

I have an AK as well.......Maadi





Actually a .460 slightly smaller bore but Highest velocity of any handgun on earth.  Great for bear and boar. And really sorry to op for this awful high jack.



:thumbup:    and    :whathesaid:


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InvisibleStuey1
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Re: G2g transfer [Re: PussyFart]
    #19308466 - 12/21/13 03:34 PM (10 years, 1 month ago)

It's all good. I think I got the info I needed


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