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OfflineCamtaro420
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My agar growth, good or bad?
    #19307586 - 12/21/13 11:40 AM (10 years, 1 month ago)

Day six since using a spore syringe. It's not in the middle(I tried to get middle but I was just trying to open and close the dishes as quick as possible in the SAB so most of them got the drops on the edges of the agar.),but I didn't innoc the middle to be fair, it is near visable spores clumps though. It's white. The pic was the best I could get and I used a magnifying glass in front of the camera.



Is it the spores germinating? Or is it cobweb? Or..... some other ungodly thing...?

Thanks for the help, just in case you're wondering none of the other petris are doing snap. Which worries me about wtf this is.


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19307637 - 12/21/13 11:48 AM (10 years, 1 month ago)

Give it some time n u should know pretty quick


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19312359 - 12/22/13 03:27 PM (10 years, 1 month ago)



Same growth the next day. Hope it's looking promising. When will it be ready for transfer if its P.C. Myc?


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19312366 - 12/22/13 03:29 PM (10 years, 1 month ago)

i transfer asap from spores, the first signs of clean growth


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19312371 - 12/22/13 03:30 PM (10 years, 1 month ago)

Err..... Does that look like clean growth lol?


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19312396 - 12/22/13 03:37 PM (10 years, 1 month ago)

kinda hard to see


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19312422 - 12/22/13 03:45 PM (10 years, 1 month ago)

Quote:

cronicr said:
kinda hard to see



Looks like I'll have to wait till tomorrow.


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Offlinenewera
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19312476 - 12/22/13 04:01 PM (10 years, 1 month ago)

what recipe did u use?


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: newera]
    #19312691 - 12/22/13 05:01 PM (10 years, 1 month ago)

That one was actually ordered pre-made for me to test against my PDYA and it's made with

21.7% Pancreatic Digest of Gelatin (Peptone G)
13% Beef Extract
65.3% Agar Agar


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19316871 - 12/23/13 04:05 PM (10 years, 1 month ago)

Got some new pictures for you guys/gals;


Zoomed


Good? Bad? Unknown?


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Offlineshaneh79
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19317517 - 12/23/13 06:08 PM (10 years, 1 month ago)

I would make a transfer if your unsure its clean. If it is white then I think things are looking good so far


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: shaneh79]
    #19317574 - 12/23/13 06:19 PM (10 years, 1 month ago)

Yep make a transfer


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I'm tired do me a favor


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InvisibleHypnotoadCroaked
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Re: My agar growth, good or bad? [Re: cronicr]
    #19318224 - 12/23/13 08:18 PM (10 years, 1 month ago)

Looks like cobweb.


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: HypnotoadCroaked]
    #19318423 - 12/23/13 08:57 PM (10 years, 1 month ago)

Quote:

shaneh79 said:
I would make a transfer if your unsure its clean. If it is white then I think things are looking good so far




Everything seems clean there is no other growth I can see in the same petri. Just that and yes in direct light it's white.

Quote:

cronicr said:
Yep make a transfer




So I should transfer now then? Where would you recommend I take from?

Quote:

Whippy said:
Looks like cobweb.




Really? I thought it was suppose to be more grey in direct light. You sure? Any other opinions on this? :frown:


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Edited by Camtaro420 (12/23/13 08:58 PM)


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19318454 - 12/23/13 09:04 PM (10 years, 1 month ago)

Tranfer the farthest leading edge


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleJ. Jack Flash
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19318491 - 12/23/13 09:11 PM (10 years, 1 month ago)

here's a couple shots of my most recent multispore squirt onto agar, for comparison.



kinda just sprayed some on the surface, swirled it around, let it set a spell, then inverted the jar.  this was about six days ago, foto from a few minutes ago.  it was really to test the jar idea, but whatever.  anyway, there's a splotch around the 11 o'clock position that looks like yours.  transfer it or wait and see what it does.


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the j stands for jesus.
2020 new years grow along


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: J. Jack Flash]
    #19319368 - 12/24/13 01:14 AM (10 years, 1 month ago)

Word thanks for that insight! Little more hopeful. What makes a good substitute scalpel? I don't have one yet.


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19319370 - 12/24/13 01:14 AM (10 years, 1 month ago)

exacto knife


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19319373 - 12/24/13 01:17 AM (10 years, 1 month ago)

Something that might be around the house?

Edit: Although there might be one around here somewhere.

Would a razor blade work ok?


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Edited by Camtaro420 (12/24/13 01:20 AM)


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InvisiblePastywhyteMDiscord
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Re: My agar growth, good or bad? [Re: HypnotoadCroaked]
    #19319520 - 12/24/13 02:26 AM (10 years, 1 month ago)

Quote:

Whippy said:
Looks like cobweb.




I was thinking the same thing. We shall see when a transfer is made.


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19319522 - 12/24/13 02:27 AM (10 years, 1 month ago)

Quote:

Camtaro420 said:
Something that might be around the house?

Edit: Although there might be one around here somewhere.

Would a razor blade work ok?



yes it will cut agar just fine


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19321314 - 12/24/13 01:54 PM (10 years, 1 month ago)

Ok so I still haven't isolated this specimen. Mainly because of how skeptical I am that it could be the AA+ spores growing. Let me run through the facts real quick.

- Out of 12 PDYA jar pertis & the 5 PBA petris only 1 has shown any type of positive growth, the one in question here in this thread.

- Inoculation occurred on 12/15 & the initial growth of said petri occurred on 12/21 (6 days after Inoc)

- Growth is white, but no rhizo growth yet after 3 days.

So those are the main concerns. Here are 3 new pictures of today's latest growth;

Non-Zoomed w/ Flash


Zoomed w/ Flash


Zoomed no Flash


Should I still try to isolate this? Or wait longer? I mean seriously no other thing in that cabinet is growing anything that looks remotely close to that. Three of the PDYA jars look like it may be growing some bacteria.... that's about it.


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InvisiblePastywhyteMDiscord
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19321560 - 12/24/13 02:42 PM (10 years, 1 month ago)

If that was mine it would be in the trash :shrug:

:thataintright:


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OfflinecronicrFacebook
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19321565 - 12/24/13 02:43 PM (10 years, 1 month ago)

Doesnt look good


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19321573 - 12/24/13 02:44 PM (10 years, 1 month ago)

Guess i'll just double bag it and continue to watch the growth for fun then.


Fuck that is so lame.


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19321585 - 12/24/13 02:46 PM (10 years, 1 month ago)

I always find it way harder with a syringe


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: cronicr]
    #19321611 - 12/24/13 02:52 PM (10 years, 1 month ago)

Yeah... Just don't seem to be that safe. Or they are hella old or something. Hear all kinds of bad things about vendor syringes. Just gotta keep on keeping on till one day I get lucky. Hopefully one of these dishes grows myc, or one of my 4 cakes grows something I can work with.


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19321633 - 12/24/13 02:57 PM (10 years, 1 month ago)

Especially pe, diffrent story when you can get a purple syringe


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleJ. Jack Flash
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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19321656 - 12/24/13 03:02 PM (10 years, 1 month ago)

Yeah looks bad, man. =frown

All mine so far have been from syringe, fwiw.  So far 1 of 4 have contaminated with a bright green dot.  The other 3 are all succeeding, touch wood.  In all cases first growth was noticeable within three days, though really difficult to see as barely noticeable dots surrounded by completely clear rings of matte finish on the glossy agar surface only visible by viewing the glare from a lamp... if that makes sense.  Two different syringes used for the sake of feeling like a bigshot.  Keep at it, man.


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: J. Jack Flash]
    #19327974 - 12/26/13 08:39 AM (10 years, 1 month ago)

So yeah, that thing is pretty much just looking more and more like cobweb, but in other news.

Check this shit out.

The other day when I used the rest of my syringe on some cakes I was getting ready to close up shop when I noticed a fucking ton of spores sitting in the cap of the thing like an hour after innocing.

So I thought to myself maybe I could do something with that. Not like I have much to lose. Looked at the petris on the shelf and thought y'know wtf ever, not like there's anything growing on these other things anyway and grabbed one of them(the cleanest of them all with no previous spores seen to the naked eye) stuck it in my SAB, opened the petri, opened cap to the syringe and banged the shit out of it to get the spores to come out.

It was not very safe, as a matter of fact I would say it was stupid as hell. banging out the spores with the lid of the petri so they would fall into the dish, but lo' and behold



Does that look like it should? Or was the cap just infested with more of whatever the hell was growing on my other dish. It's been three days since the spores hit that dish


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19328098 - 12/26/13 09:35 AM (10 years, 1 month ago)

Yeah that is too quick and don't look right to me, sorry. The growth is very random, even with a lot of spores, cube growth should be somewhat directional.


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: Pastywhyte]
    #19328138 - 12/26/13 09:48 AM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Yeah that is too quick and don't look right to me, sorry. The growth is very random, even with a lot of spores, cube growth should be somewhat directional.




:smokerage:

G** F****** D*****


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19328150 - 12/26/13 09:52 AM (10 years, 1 month ago)

Don't pitch it just yet tho, there is a lot of spores on that plate, far more than I would ever use. Maybe its just cause there are so many that it looks so random. Give it another day and see how it looks then.


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OfflineCamtaro420
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Re: My agar growth, good or bad? [Re: Pastywhyte]
    #19328162 - 12/26/13 09:57 AM (10 years, 1 month ago)

Word man thanks for the help. Just to be fair it was not sterile procedures at all other then a SAB.

I was very skeptical anything good would come from that one.


I followed my BRF cake PF TEK to a tee so all my hope is resting on one of my four cakes to colonize and pin so I can get something to work on agar with.

I'll have more posted tomorrow.


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Re: My agar growth, good or bad? [Re: Camtaro420]
    #19328447 - 12/26/13 11:27 AM (10 years, 1 month ago)

Fuck the print clone buddy! I mean take prints but clone!


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I'm tired do me a favor


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InvisibleHypnotoadCroaked
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Re: My agar growth, good or bad? [Re: cronicr]
    #19329386 - 12/26/13 03:44 PM (10 years, 1 month ago)

Agar is great for prints, but it sucks if you are using a liquid MSS.  If I need to move from MSS to agar, I will make a plate or 2 of "brf agar".  It takes the additional fluid much better than the traditional agar-agar.

Otherwise, odd looking growths at the edges of plates are generally not good.


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