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WillSolvem
Odd-Hand



Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Tetraploid shrooms? [Re: dusttodust]
#19237303 - 12/06/13 04:24 PM (10 years, 2 months ago) |
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AMU Q&A Thread because questions deserve answers.
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Topcorn
False idol



Registered: 02/02/07
Posts: 271
Loc: Observable universe
Last seen: 3 months, 9 days
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Re: Tetraploid shrooms? [Re: Asante]
#19241846 - 12/07/13 05:19 PM (10 years, 2 months ago) |
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Quote:
Wiccan_Seeker said: Back in the 1980s, herb was herb. Then cultvators came along who with use of colchicine produced tetraploid cannabis, they called it Skunk, and with it laid the foundation for the superweed we have today.
You are wrong, ever heard of selective breeding? People who think potent strains of cannabis did not exist until after the 60s/70s are just spewing bullshit. I am not saying much hasn't been improved in the last few decades but that was the result of many peoples hard work in searching for new genetics and testing seed after seed for the rare gems that made up most of the gene pool we smoke today.
Much can be done with selective breeding. For instance, corn was bred from regular sized grain ancestors and broccoli, cabbage, brussel sprouts, cauliflower, kale and kohlrabi all were bred from a single ancestor(Brassica, wild mustard). Yes these took a long time to come about and in the case with cannabis there seems a steep rise in quality of genetics over just decades. But with cannabis we haven't done much but combine naturally occurring high cannabinoid plants with each other, the sudden flood of quality resulted from the sudden rise in numbers of people searching for quality and growing their own shit.
-------------------- Slayer of robot dinosaurs in a futuristic landscape
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Tetraploid shrooms? [Re: Topcorn]
#19241949 - 12/07/13 05:48 PM (10 years, 2 months ago) |
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-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Amanita virosa
botanist by day


Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: Tetraploid shrooms? [Re: Topcorn]
#19242149 - 12/07/13 06:44 PM (10 years, 2 months ago) |
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Quote:
Topcorn said:
Quote:
Wiccan_Seeker said: Back in the 1980s, herb was herb. Then cultvators came along who with use of colchicine produced tetraploid cannabis, they called it Skunk, and with it laid the foundation for the superweed we have today.
You are wrong, ever heard of selective breeding? People who think potent strains of cannabis did not exist until after the 60s/70s are just spewing bullshit. I am not saying much hasn't been improved in the last few decades but that was the result of many peoples hard work in searching for new genetics and testing seed after seed for the rare gems that made up most of the gene pool we smoke today.
Much can be done with selective breeding. For instance, corn was bred from regular sized grain ancestors and broccoli, cabbage, brussel sprouts, cauliflower, kale and kohlrabi all were bred from a single ancestor(Brassica, wild mustard). Yes these took a long time to come about and in the case with cannabis there seems a steep rise in quality of genetics over just decades. But with cannabis we haven't done much but combine naturally occurring high cannabinoid plants with each other, the sudden flood of quality resulted from the sudden rise in numbers of people searching for quality and growing their own shit.
And Lets not forget what 10,000 years of artificial selection did to the dog
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WillSolvem
Odd-Hand



Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Quote:
Amanita virosa said:
And Lets not forget what 10,000 years of artificial selection did to the dog
Thankfully mushroom generations are much shorter
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AMU Q&A Thread because questions deserve answers.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Re: Tetraploid shrooms? [Re: WillSolvem]
#19244220 - 12/08/13 09:57 AM (10 years, 2 months ago) |
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I have a triploid cannabis plant which occurred naturally on the third generation since I got it. It's a dj short strain, and they're advertised as organic. It's the only triploid plant I've ever seen so it will be interesting to see what comes of it. The original ten seeds were from his blueberry sativa crossed to his blueberry indica. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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g3n3h4x0r
Fungal Geneticist

Registered: 09/29/13
Posts: 73
Last seen: 8 years, 10 months
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Let me know if you test this. I have an Agro transform I'm doing in a few months, and it would be ridiculous if I could induce pluripotency afterward. It would be a whole new strain.
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shopdropper
Professional Psychonaut


Registered: 03/13/06
Posts: 1,623
Loc: Sailing the sea of cheese
Last seen: 5 days, 13 hours
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Re: Tetraploid shrooms? [Re: g3n3h4x0r]
#19247990 - 12/08/13 11:32 PM (10 years, 2 months ago) |
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that's really sweet. dj short was the originator of the blueberry strain correct? id like to see some pics of this triploid plant! did it start out with 3 leaves?
-------------------- DON'T TRY THIS AT HOME: no guarantees can be made about the accuracy of the information herein. The information dicussed in these posts is purely hypothetical, and for intelectual purposes only. Any similarity between internet chat and real life is pure coincidence.
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caricapapaya
Stranger



Registered: 04/10/06
Posts: 258
Last seen: 1 year, 9 months
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"I have a triploid cannabis plant which occurred naturally on the third generation since I got it. It's a dj short strain, and they're advertised as organic. It's the only triploid plant I've ever seen so it will be interesting to see what comes of it. The original ten seeds were from his blueberry sativa crossed to his blueberry indica. RR"
How do you know it is triploid?
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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My question is; how would you test for a tetraploid mushroom? Am I looking for a two foot high mushroom, or is it going to be respectively denser tissue? Or something only observable under magnification? Can it be observed at all? I apologize if I'm missing something obvious, this is very interesting and I agree worth the effort. I would just like to know the acceptable measurement of success.
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AMU Q&A Thread because questions deserve answers.
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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Re: Tetraploid shrooms? [Re: teknix]
#19288108 - 12/17/13 11:23 AM (10 years, 2 months ago) |
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Quote:
teknix said: Why are you guys still calling it haploid and diploid? No such thing in the kingdom of fungus.
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Tetraploid shrooms? [Re: urthtown]
#19306069 - 12/21/13 01:46 AM (10 years, 2 months ago) |
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-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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spore baby



Registered: 07/30/13
Posts: 4,918
Last seen: 8 years, 7 months
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Re: Tetraploid shrooms? [Re: Asante]
#19306129 - 12/21/13 02:18 AM (10 years, 2 months ago) |
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Edited by spore baby (12/13/14 04:07 AM)
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Tetraploid shrooms? [Re: spore baby]
#19307633 - 12/21/13 11:48 AM (10 years, 2 months ago) |
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Well the good news is that we have people like Alan Rockefeller and Workman who actually have degrees and licenses to work with psychoactive mushrooms. If you did come close to pulling this off, I bet either of those guys would jump on the chance to do some analytical work, or have it done.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Icyus
KavitārkikasiṃHa



Registered: 11/07/13
Posts: 3,502
Loc: Inbetween.
Last seen: 8 years, 1 month
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Re: Tetraploid shrooms? [Re: 36fuckin5]
#19307646 - 12/21/13 11:50 AM (10 years, 2 months ago) |
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-One uses torture to become stronger and then either one comes out with tremendous power, or broken diagonally..
-------------------- And thus begins the reverse-fusing of our one-dimentional understanding, and adds ever-expanding perspectives, in depth and number; splitting our perception, and in so doing, seemingly irrationally, creates yet more one-ness, with all that ever was, is and will ever be, streching across the infinite, inunderstood concept of everything, percievable and not.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Tetraploid shrooms? [Re: spore baby]
#19310079 - 12/22/13 12:18 AM (10 years, 2 months ago) |
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Quote:
spore baby said: Unless you have access to a genetics laboratory there will be no way for you determine if it even worked.
Sending mushrooms to a government laboratory to get tested will be a bad idea. 
Karyotyping is not difficult. All you need is some myc on a slide, the right stain, and a good scope.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Tetraploid shrooms? [Re: fastfred]
#19311184 - 12/22/13 09:43 AM (10 years, 2 months ago) |
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Quote:
fastfred said:
Quote:
spore baby said: Unless you have access to a genetics laboratory there will be no way for you determine if it even worked.
Sending mushrooms to a government laboratory to get tested will be a bad idea. 
Karyotyping is not difficult. All you need is some myc on a slide, the right stain, and a good scope.
-FF
What am I looking for under these conditions?
Edit: found information on Google... wow, very interesting stuff
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AMU Q&A Thread because questions deserve answers.
Edited by WillSolvem (12/22/13 09:47 AM)
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aris
Feline Bovine

Registered: 07/20/10
Posts: 715
Loc: dl
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: Tetraploid shrooms? [Re: aris]
#19335410 - 12/28/13 02:48 AM (10 years, 1 month ago) |
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"Karyotype analyses are performed over 400,000 times per year in the U.S. and Canada." "A karyotype analysis usually involves staining the condensed chromosomes with Giemsa dye."
Just dye them and scope them!
No need to fix and section them with a microtome since you should be able to get the myc network growing in a single layer.
Finding a cell in the right stage of mitosis should not be terribly difficult if you use an actively growing culture.
Doesn't get much easier than this for genetic analysis techniques!
-FF
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That Man
Common Goer



Registered: 02/09/14
Posts: 173
Last seen: 9 months, 10 days
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Re: Tetraploid shrooms? [Re: 36fuckin5]
#19849791 - 04/15/14 04:56 PM (9 years, 10 months ago) |
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Quote:
36fuckin5 said:
Very interesting!
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