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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Another first time grow
#19304576 - 12/20/13 07:07 PM (10 years, 2 months ago) |
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My first post on here. This is yet another first timer grower looking for feedback and advice. I lurked for about a month before starting anything, then ordered some syringes from a popular non-sponsor vendor. I just want to grow for personal consumption, maybe two times a year or so. Also of note, I like to experiment for no reason other than it makes this more fun.
I started off with some Cambodian cube spore syringes, and inoculated a custom liquid culture in a re-purposed salsa jar. I didn't have potatoes, so I boiled up some oatmeal, took off ~half a cup of the liquid to use, added more water, and corn syrup to ~3-4% final. I also PC'd all jars used here appropriately (I think). This is one week of growth with 1cc of Cam spore syringe:

I also made a (similar formula) Agar streak plate, and stuck it in the fridge after 4 days for later isolations, if I am feeling ambitious. I think I need to do a better job with this.

I used this liquid culture to inoculate some 1 pint jars of slightly modified BRF cakes. I definitely didn't break up the myc in the LC very well before I injected 15cc into each jar, but I think I learned my lesson. My recipe was:
• 0.25 cup Gypsum • 1 cup Spent coffee grounds • 0.5 cup Flax meal • 5 cup Brown rice flour • 10 cup Vermiculite • 5 cup Water (add slowly, make sure its not too wet)
I didn't mix them that well I guess, some of the whiter parts are flour, and darker parts are coffee... and this is how they look after 4 days of growth at ~27C.
 
I used the self-healing injection port tek extensively for everything, and some micropore sort of surgical tape.

I also wanted to use up some things that have been sitting around unused in my kitchen, so I inoculated 1 cleaned out pickle and 1 salsa jar with a custom spawn recipe consisting of quinoa, flax meal, psyllium husk, and vermiculite. This was just for kicks. This is those jars 4 days later.. I may have added too much water, as these jars seem a bit too wet.

And that's about it for now. I'm not sure where I am going next, likely going to spawn them to a standard coir/verm/gypsum monotub if everything goes alright! I expected a high jar failure rate, since this is my first time, but everything is going OK so far as I can tell. Is this too many jars for one tub? Any advice or comments welcome.
Edited by kaptanoblivious (12/21/13 03:27 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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No thats about five qts, and 2/3s of those jars are verm
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 4 months
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Pretty ambitious! I hope you will update this thread as I am interested in seeing how everything turns out for you.
What did you do with the lids of the repurposed jars? I am also using some washed out pasta sauce jars with RTV and tyvek filters.
Welcome to the Shroomery and good luck!
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Quote:
kratom_redmomd said: What did you do with the lids of the repurposed jars?

For the salsa-jar LC I just used a single RTV injection port, no gas exchange at all.
For the weird spawn jar creations, I did the same as the picture, 4 RTV injection ports and a gas exchange micropore tape hole in the middle. Hopefully nothing blows up.
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Edited by kaptanoblivious (12/21/13 03:31 AM)
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 4 months
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Quote:
kaptanoblivious said: For the salsa-jar LC I just used a single RTV injection port, no gas exchange at all.
For the weird spawn jar creations, I did the same as the picture, 4 RTV injection ports and a gas exchange micropore tape hole in the middle. Hopefully nothing blows up.
Right on. Sending good vibes your way!
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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SgtPepperNo9
Stranger


Registered: 10/30/13
Posts: 364
Last seen: 9 years, 9 months
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Looking good man! 
You definitely dove in head first with the LC and agar work. Plus with the custom substrate mixture. If it all works out then congrats! I experimented with my first too but not to the extent you did. Your mix seems a lot darker then mine did but I am thinking that is maybe because of the coffee grounds.
I'm also curious about something. Why use the LC first when you could shoot them up from a sterilized syringe that you know is free of any contam? It seems like the more processes you add the more chance for contam. I understand the LC to get more from your syringe and make it last longer but I don't know.
I will be watching your progress, I'm interested to see how everything turns out.
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Quote:
SgtPepperNo9 said: I'm also curious about something. Why use the LC first when you could shoot them up from a sterilized syringe that you know is free of any contam? It seems like the more processes you add the more chance for contam. I understand the LC to get more from your syringe and make it last longer but I don't know.
I have a few syringes, but yeah, everything I have I only used 1cc of one syringe, so it was to stretch it out... I guess I wasn't thinking about my chances of contamination. I am pretty confident with sterile technique, so I maybe foolishly assumed the added risk was minimal. Will definitely consider this in the future.
Another weird thing, I had gotten another strain from the same vendor, and tried to make an LC and an agar plate with that one too, but no growth yet after 11 days.
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jsad316
Boost whore



Registered: 03/07/12
Posts: 114
Loc: Texas
Last seen: 9 years, 4 months
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This first grow > my first grow. So much more advanced. I'm familiar with liquid cultures but not with agar. But I kept it simple as hell and had great success. Hope yours turns out even better (as it should)!
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Don't stop 'till the music does.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Another first time grow [Re: jsad316]
#19304951 - 12/20/13 08:13 PM (10 years, 2 months ago) |
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I'd let that plate do some growing
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: cronicr]
#19305060 - 12/20/13 08:40 PM (10 years, 2 months ago) |
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Quote:
cronicr said: I'd let that plate do some growing
I was hoping to use it to pick some clonal isolates to make agar slants. If I let it grow longer, won't the colonies grow together? Maybe I should read up on getting isolates, I made a few assumptions here.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Oh k i see, if u got plates do it now to keep busy
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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SgtPepperNo9
Stranger


Registered: 10/30/13
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Last seen: 9 years, 9 months
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Quote:
kaptanoblivious said: I was hoping to use it to pick some clonal isolates to make agar slants. If I let it grow longer, won't the colonies grow together? Maybe I should read up on getting isolates, I made a few assumptions here.
Pretty impressive talk going on in here for 1st grow. Do you have a background in biology or something related to science? I'm excited for you!
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Yes I have a biology background --- but fungus is usually contamination for me
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Edited by kaptanoblivious (12/21/13 12:15 AM)
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SgtPepperNo9
Stranger


Registered: 10/30/13
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That's what it seemed like. I am about to order up some agar myself. I was pretty interested in micro when I took a couple classes on it. The labs were really fun for me and I did well in them because of that. I rediscovered that interest in this hobby. I wish I had been into it back then, full walk in incubators, warm/cold rooms, bunsnen burners, microscopes, unlimited agar and petri dishes. We kind of had the freedom to do what we wanted to and check on them outside of class. Grow random stuff just to make slides, stain them and check them out.
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 6 months, 14 days
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crackacola
Big Daddy


Registered: 12/14/13
Posts: 234
Last seen: 8 years, 10 months
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Re: Another first time grow [Re: vikingsc]
#19306201 - 12/21/13 02:53 AM (10 years, 2 months ago) |
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Awesome job on your first LC!
You put 15cc into EACH JAR? Or spread out through all your jars?
That is not micropore tape. That is biopore or something like that. It will say inside the roll of the tape. May or may not work, IDK.
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FOR TRADE <---- Check out my spores for trade!
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: crackacola]
#19306255 - 12/21/13 03:23 AM (10 years, 2 months ago) |
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yeah, I put 15cc into each jar. I thought that I saw on another thread someone put 10cc of LC per smaller jar... idk I just picked an amount that used most of my LC. too much? 
I think you are right about the tape... it does not explicitly say micropore on it... good eye. It is a cloth-type surgical tape. well, part of the learning process I guess. There's a thin dry verm layer at least, so I'll see how it plays out. Not sure there is FAE now though.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Its 1cc per jar
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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Re: Another first time grow [Re: cronicr]
#19306266 - 12/21/13 03:30 AM (10 years, 2 months ago) |
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Quote:
cronicr said: Its 1cc per jar
oops
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Edited by kaptanoblivious (12/21/13 03:33 AM)
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kaptanoblivious
Moron


Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
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So is using way too much LC going to hurt my jars? Or did I just waste a bunch of LC, which doesn't bother me too much.
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