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Offlineinvitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
Lc questions
    #19303950 - 12/20/13 05:11 PM (10 years, 1 month ago)

When you go to pc a liquid culture, the water inside boils right?  If so, does it matter if it splashes up to the sfd, or whatman filter?  Also can a LME/DEX solution carmelize if overcooked?

Thanks guys!


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OfflinePyropastie
Stranger


Registered: 11/10/13
Posts: 12
Last seen: 9 years, 10 months
Re: Lc questions [Re: invitro]
    #19303976 - 12/20/13 05:18 PM (10 years, 1 month ago)

The filter will be fine, not sure about overcooking dex though. Although most liquid cultures don't need pcing for very long so if it does caramelise just put it in for less time.


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InvisibleWilly Wonka
Medicine Man
 User Gallery


Registered: 12/14/11
Posts: 2,109
Loc: The Other Side Flag
Re: Lc questions [Re: Pyropastie]
    #19304300 - 12/20/13 06:14 PM (10 years, 1 month ago)

30min at 15psi should be fine.


--------------------
AMU



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InvisibleFred Teddy
Flying with Turkeys


Registered: 07/18/11
Posts: 208
Re: Lc questions [Re: invitro]
    #19306989 - 12/21/13 09:00 AM (10 years, 1 month ago)

Quote:

invitro said:
When you go to pc a liquid culture, the water inside boils right?  If so, does it matter if it splashes up to the sfd, or whatman filter?  Also can a LME/DEX solution carmelize if overcooked?

Thanks guys!




Most sugars carmelize at well over the temps we use, over 300F. But, fructose (corn syrup/karo) needs care - carmelizes at 230F. 30 min tops in pc for fructose LC's. Dextrose (glucose) is 320F. No worries there.

Peace,
FT


--------------------
... its all about fun and games ... and no one has poked their eye out ... yet.

Peace.

Disclaimer: Fact is ... Its all fiction.


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