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listerine
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Registered: 10/10/12
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
#19301635 - 12/20/13 06:58 AM (10 years, 2 months ago) |
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Maybe I underpasteurized it. check out the tek i used. Sounds legit? turn off the water when it comes to a boil, cover and leave for 2-3 hours.
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spacechildo
proletarians rise up



Registered: 01/24/13
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
#19301643 - 12/20/13 07:02 AM (10 years, 2 months ago) |
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sounds awfully wrong. get core temp to 140-160 for 1hr. that's what the thermometer is for, to keep track of core temp. the temp of the water makes no difference, it's the poo that needs 140-160 for 60-90 mins roughly.
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Aero
Orea


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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301651 - 12/20/13 07:04 AM (10 years, 2 months ago) |
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Quote:
spacechildo said: sounds awfully wrong. get core temp to 140-160 for 1hr. that's what the thermometer is for, to keep track of core temp. the temp of the water makes no difference, it's the poo that needs 140-160 for 60-90 mins roughly.
doesnt matter if u over-past the sub mate u only kill more beneficial bacteria, u will get higher chances of contam but wouldnt make any difference in wether it colonizing the sub or not and in this thread this is the case now..so back up baby we all know what is pasteurization
-------------------- SPREAD THE SPORES
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301659 - 12/20/13 07:06 AM (10 years, 2 months ago) |
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How could i pasteurize without a thermometer? With the same set up if possible//
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spacechildo
proletarians rise up



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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19301666 - 12/20/13 07:11 AM (10 years, 2 months ago) |
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Quote:
listerine said: How could i pasteurize without a thermometer? With the same set up if possible//
I really don't know. try switching poo for coir, it's more forgiving and you can try the bucket-tek. it doesn't seem to matter that much if you don't prep it correctly, I've seen good results with both sterilization and no pasteurization with coir. There's no way to tell how hot the core of your sub is vs the heat of the water. some use bags, some use jars, size of bags/jars makes a difference.
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Aero
Orea


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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301670 - 12/20/13 07:12 AM (10 years, 2 months ago) |
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like what he said, coir is easier to work with, although u can try to boil the water put in the sub then leave it on the lowest gas/electricity level for 2 hours
i doubt that the pasteurization is the reason why it didnt colonize
-------------------- SPREAD THE SPORES
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301780 - 12/20/13 07:56 AM (10 years, 2 months ago) |
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Someone recommended the bucket tek with manure/straw. Any issues?
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
#19301781 - 12/20/13 07:57 AM (10 years, 2 months ago) |
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>i doubt that the pasteurization is the reason why it didnt colonize
What do you think it could be then?
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spacechildo
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19301796 - 12/20/13 08:03 AM (10 years, 2 months ago) |
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dont do bucket tek with anything besides coir+verm and gypsum. it's most likely your spawn, but the improper pasteurization certainly didn't help..
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301804 - 12/20/13 08:04 AM (10 years, 2 months ago) |
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What do you think is wrong with my spawn?
It hasn't been incubated for a few days, but fully colonized before that. WBS.
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spacechildo
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19301810 - 12/20/13 08:07 AM (10 years, 2 months ago) |
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some contam in the spawn is probably why it won't recuperate. some pics would def. help, did u smell every jars before spawning?
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: spacechildo]
#19301812 - 12/20/13 08:09 AM (10 years, 2 months ago) |
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I don't think its a contam. This has happened before, different spawn jars, visual inspection, I don't smell the jars but yeah.
I soak them in cold tap water. I think ill cut that out.
Why can't i do the bucket tek with manure/straW?
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tga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
#19301819 - 12/20/13 08:12 AM (10 years, 2 months ago) |
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With your pasteurization tek if your using a thin pot with a loose lid once you turn off the heat you may be getting the sub to proper temp but highly doubt its staying hot long enough. But if you use a pressure cooker like i did recently using a thermometer I brought it to a boil shut it off put lid on and checked 30 minutes later sub(coir) temp was up to 150 and the water was down to 150. So I brought it back to a short boil and removed keeping the lid on tightly while off the heat and an hour later checked again it was still 150 perfect!
Did you do the squeeze test for field capacity and compare it to RRs video? even with a hard squeeze you shouldn't drip alot.
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Fred Teddy
Flying with Turkeys


Registered: 07/18/11
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
#19301865 - 12/20/13 08:31 AM (10 years, 2 months ago) |
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It sounds more like a bacterial thing to me, but let it go till it is obvious its truly stalled.
Could also be ammonia left in the sub after pasteurization. 2 week old manure is a bit ripe imo. 'New' manure should be leached well before preparing, or avoided altogether. It should look the color of a paper bag or lighter. The older the better.
Here's something on the decomposition of horse manure: http://www.horsekeeping.com/horse_management/composting.htm
Peace, FT
-------------------- ... its all about fun and games ... and no one has poked their eye out ... yet. Peace. Disclaimer: Fact is ... Its all fiction.
Edited by Fred Teddy (12/20/13 08:48 AM)
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spacechildo
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19301867 - 12/20/13 08:32 AM (10 years, 2 months ago) |
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Quote:
listerine said: I don't think its a contam. This has happened before, different spawn jars, visual inspection, I don't smell the jars but yeah.
I soak them in cold tap water. I think ill cut that out.
Why can't i do the bucket tek with manure/straW?
because manure and just about everything except coir+verm+gypsum needs to be properly pasteurized. properly pasteurized means 140-160F for an hour or so. bucket tek is just boiling water right into a bucket. you need to monitor temps, which is (as far as I can tell) impossible without a thermometer.
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Psychoslut
The Mother Fucking Bear-o-dactyl


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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19302036 - 12/20/13 09:31 AM (10 years, 2 months ago) |
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A thermometer is about 3.50, just go get one.
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[quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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tga8706
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Re: Colonized WBS won't colonize h.manure [Re: Psychoslut]
#19302160 - 12/20/13 10:10 AM (10 years, 2 months ago) |
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Quote:
Psychoslut said: A thermometer is about 3.50, just go get one.
He can't get one in his country, evidently he lives in antartica or something.
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Psychoslut
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19302206 - 12/20/13 10:28 AM (10 years, 2 months ago) |
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I'm guessing its bacteria stopping it from colonizing. It probably stayed hot too long in the bag. Give it more time, the grain should at least brightening up and starting to reach out to the substrate by now.
If the grain isn't recovering at all this grow is probably fucked from a few different directions.
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[quote]KristiMidocean said: Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]
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RogerRabbit
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
#19302306 - 12/20/13 10:55 AM (10 years, 2 months ago) |
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I suspect contaminated spawn. I see no reason the horse manure wouldn't colonize if it were anything else. Even if it's a bit dry or too wet, it should colonize at least some.
I suspect bacteria infection in your spawn. Give the smell test every jar before using. One bad jar of spawn in a bulk substrate, even if the other grain spawn was OK, can ruin it all. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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listerine
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Re: Colonized WBS won't colonize h.manure [Re: RogerRabbit]
#19302413 - 12/20/13 11:22 AM (10 years, 2 months ago) |
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Well i got this from a spore print, transfered to a petri dish, then once more. From there a liquid culture, which I know you hate. I have done maybe 30 grain jars from the LC, and 4 of them became contaminated over time. some Black contam.
But the grain spawn that i did use, looked fine visually.... There was a jar which had a bit of yellow water in one area, not sure if that was a contam or just metabolites, but i didn't use that. My sterilization protocol, I think is pretty good, as I have some training in that area.
I don't know what to do. This has happened once before.
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