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OfflineOregonMushys
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Registered: 09/09/13
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Re: G2G TimeLapse Ps. Cubensis [Re: OregonMushys]
    #19176222 - 11/22/13 07:35 PM (10 years, 3 months ago)

EDIT: they WERE NOT CONTAMS and i had 100% success. every jar smelled fresh mushrooms. g2g ftw

well, to also show all the n00bs out there lol.. i think i got contams in some of the jars. i expected it since it was my first g2g. its mycelial structures forming on the glass near the top of the jar where there is no grain at whatsover, just condensation. at

at first i thought, could some cube mycelium have rubbed off onto the glass from shaking it so hard? most likely not... ill mark every suspect jar and keep seperate. so timelaps is over since i moved the jars all around and some r contamd.

i'll keep updating. we'll see if i get that measly 50% i was mentioning. :lol:

Heres pics of the suspect contams


EDIT: they WERE NOT CONTAMS and i had 100% success. every jar smelled fresh mushrooms. g2g ftw


Edited by OregonMushys (12/20/13 08:53 AM)


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OfflineOregonMushys
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Registered: 09/09/13
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Re: G2G TimeLapse Ps. Cubensis [Re: OregonMushys]
    #19301901 - 12/20/13 08:41 AM (10 years, 2 months ago)

Well, the suspect contams were actually cubensis myc and i had 100% success every jar smelled like fresh mushrooms. They fully colonized in 7-14 days and ima have to make a full on timelapse next time around. I kept moving the jars around so i stopped takin pics.

Agar & g2g ftw.


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Ps. Cubensis                                        Ps. Cyanescens                                      Ps. Stuntzii  *GrowLog*
   
                                                                                                      :sasquatch::mushroomgrow::leaf::mushroomgrow::leaf::mushroomgrow:
                                                                                                  :cop:


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Offlineloki44
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Re: G2G TimeLapse Ps. Cubensis [Re: OregonMushys]
    #19301970 - 12/20/13 09:10 AM (10 years, 2 months ago)

GLC is:

-faster( you need 1/3 of a half pint jar to colonize against 1 quart jar on G2G

-more efficient( out of one 500ml LC you can inoculate 100's of jars no need to do multiple transfer weakening the mycelium because of senescence

-less rate of contamination since the exposed area of a needle is hundreds of times smaller than the exposed areas of both your master jar and the receiving jar when you are doing the deed

easier- less skill required, just flame the needle get your syringe in the SAB , stick it on the inj port and squeeze

:justno:


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InvisiblePastywhyteMDiscord
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Re: G2G TimeLapse Ps. Cubensis [Re: loki44] * 2
    #19302066 - 12/20/13 09:41 AM (10 years, 2 months ago)

G2G in a nutshell

1 quart master Inoculated with agar wedge
G2G to 10 quarts for P1
G2G those to 100 quarts for P2
G2G to 1000 quarts for P3
G2G to 10000 quarts for P4

10000 quarts, no risk of senescence, reliable and clean. I can't see a GLC being the better alternative, sorry. Only issue would be finding and storing 10000 jars. Also a syringe has far more risk for contamination than G2G because the needle actually makes contact with the inside of the spawn jar. In a proper SAB the biggest vector for spreading contams is touch, not the exposed medias surface area. This actually makes G2G far safer. I have never had a contam in a reciving jar yet though I only do about 2 dozen a month.


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Offlineloki44
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Re: G2G TimeLapse Ps. Cubensis [Re: Pastywhyte]
    #19302126 - 12/20/13 09:59 AM (10 years, 2 months ago)

pasty,

1.what is faster to colonize: one  third of a half pint jar or one quart jar worth of grains , provided that you use the same size of agar wedge from a clean monoculture?

2.most people dont need 10k jars of grains, once you have your clean GLC, you dont need to waste more time doing more transfers and degrading your mycelium!

logic prevails :tongue2:


Edited by loki44 (12/20/13 10:00 AM)


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OfflineOregonMushys
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Re: G2G TimeLapse Ps. Cubensis [Re: loki44]
    #19302188 - 12/20/13 10:23 AM (10 years, 2 months ago)

I typed up a storm but its a must read. :bongload::mushroomgrow:

Its all about agar/G2G/spawnbags. Ive had success with GLC, but ive also had a lot of random happenings. Such as i inoculate like 100 jars, wait a month and see nothing(weird ha this is why i moved on to g2g). Or wait a month and find contams in a lot of them. With GLC, you dont for sure know what your dealing with until you noc something up with it first to see, and that takes a tester month. When that month of GLC test time is up, one could have already had many master jars colonized from agar wedges. Once you have fully colonized masters, it'll only take 6 weeks max to get thousands of qts of colonized spawn, depending how you go about it.

The process of aspirating the GLC out into a syringe every time is a chance you can invite something into the ENTIRE culture. So the process of inoculating a tester jar itself, adds another chance to contam the entire culture you're working with. If some type of contam does get invited in it your GLC, theres no smelling the GLC. With g2g, you can easily see the master culture before the g2g process, and easily smell it right after the g2g process is finished to see if it smells funky or not.

With G2G, you can fuck up while pouring one jar and might invite contams, but thats that one jar you had a fuck-up on. With each jar is a different possibility if you invite contams or not. When using GLC, you just gotta inoc all your jars with one sketchy culture you dont for sure know about since you cant easily visualize it nor smell it and you had to stick many needles in that port so you know you have highered the chances of pushing something in there. The point is, you cant see/smell possible contams with GLC and if it goes bad, its the entire culture and all that wasted time. With g2g you can easily see/smell the donor culture jar, and the chances of inviting contams are variously individual from jar to jar whereas GLC is one sketchy culture to every single jar.



And tbh, ive found that 1/3 of a pint jar of grains takes just about as long as a qt jar of grains would to colonize. Maybe a week quicker.


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InvisiblePastywhyteMDiscord
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Re: G2G TimeLapse Ps. Cubensis [Re: OregonMushys]
    #19302639 - 12/20/13 12:13 PM (10 years, 2 months ago)

Quote:

loki44 said:
pasty,

1.what is faster to colonize: one  third of a half pint jar or one quart jar worth of grains , provided that you use the same size of agar wedge from a clean monoculture?




I personally use pints for my masters as they are easier to work with in the SAB for my initial G2G. The speed may be a little faster but not so much that I would bother with less than a pint for a grain master. I usually only G2G once as its easy to get a clean culture, transfer to a bunch of plates then use those to do up half a dozen pint masters. Half a dozen pint masters will easily nock up 35-40 quarts, more than enough for my needs.

Quote:

loki44 said:
2.most people dont need 10k jars of grains, once you have your clean GLC, you dont need to waste more time doing more transfers and degrading your mycelium!




Of course they don't. The purpose was to illustrate how fast a culture on grains can be expanded. Senescence is not an issue for the most part until after transfer P5 or P6, by which point you would have 100000 -1000000 quarts of spawn. This could be done in about 6-8 weeks from the point of the master inoculation. If you were to use GLC to reproduce this rate of expansion, you would need 10000 masters to make that much GLC to start with.

Think of it like this. Do up 6 pints as masters and G2G them to 36 quarts. You should have colonization in 1-1.5 weeks depending on temp, GE, genetics, etc. Now if you are doing 5 monotubs and you want 5 quarts per tub that leaves you with 6 quarts. Take those 6 quarts and G2G those to up to 72 quarts. Now while your tubs are colonizing and fruiting you still have a supply of twice as much spawn at your disposal. My biggest problem right now is having more spawn than I know what to do with :lol: I don't like having more than 24-30 quarts worth of spawn in subs fruiting at a given time, just don't have the space. So my fridge ends up full of cultures on grains that I hope to get to in a few weeks :super: Every now and then I forget stuff and that always feels like a waste.

IME senescence is not even that much of an issue with any culture that has not been taken from a fruitbody. You would need to be careful and rotate media with clones for that reason if you want to keep them around for a long time, but I have a feeling that the recommended P5 - P6 transfers are a lowball minimum for MS or a true isolate.

Quote:

OregonMushys said:
With G2G, you can fuck up while pouring one jar and might invite contams, but thats that one jar you had a fuck-up on. With each jar is a different possibility if you invite contams or not.




Sure is and that's the beauty of it. Don't take many tries before you get really good at it and have a 0%-5% fail rate due to procedure. Once you have that working and have your agar down (and there is a learning curve to spot contams on agar, sometimes the contams can be sneaky, but again practice makes perfect), its easy to maintain a success rate of 95% or higher. This is IMO a prerequisite to learn proper sterile tek in order to have success with any liquid culture as an inoculate for grain media.


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OfflineSynKyd
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Re: G2G TimeLapse Ps. Cubensis [Re: Rubestoad]
    #19302729 - 12/20/13 12:33 PM (10 years, 2 months ago)

Quote:

Rubestoad said:
Quote:

OregonMushys said:
It just seems hella hard to pour grains from a bag, though if the culture isn't weakening and you have some legit sterile way to g2g from a bag then go for it.





take bag cut a 1/2 inch or less chunk out of one corner and g2g in a sab with it.

its just so easy it might work




Big fan of G2G.........My current grow is a G2G that started in bags.  Wasn't a plan, it just worked out that way.

I did the transfer in SAB, cut bag open and used a alcohol-sanitized spoon to move the grains to jars.  0 contams and it's now fruiting.

I know it's not sterile, probably not recommended, but it did work for me for spawn bags bought online as a noob.


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OfflineSporecupine
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Re: *G2G TimeLapse Thread* [Re: OregonMushys]
    #24210632 - 04/01/17 11:33 AM (6 years, 10 months ago)

Useful visual for a total noob like myself. It's helpful to know what's going to happen, and how it should look.


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