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Offlinelisterine
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Colonized WBS won't colonize h.manure
    #19300984 - 12/20/13 01:52 AM (10 years, 2 months ago)

What could be wrong? Monotub has a bit of g/e, I followed RR's video instructions, additionally I soaked the spawn grain in water, for 20 mins.

Its 50/50, manure/straw. roughly field capacity. condensation in the tub. Covered with Aluminum foil, with holes poked.

This is the second time that I have screwed up at this stage. I don't know what im doing wrong. Ideas?


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OfflinecronicrFacebook
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
    #19301024 - 12/20/13 02:11 AM (10 years, 2 months ago)

How long has it been,what are the temps,roughly at field capacity sounds iffy lol
Where did the poo come from


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Edited by cronicr (12/20/13 03:12 AM)


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: cronicr]
    #19301278 - 12/20/13 04:09 AM (10 years, 2 months ago)

what was the poo temp when u mixed in the wbs?


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301281 - 12/20/13 04:10 AM (10 years, 2 months ago)

u are not supposed to soak the spawn grain as u can wash off the mycelium
and roughly field capacity is not field capacity
some pictures would help though


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301299 - 12/20/13 04:20 AM (10 years, 2 months ago)

Quote:

Aero said:
u are not supposed to soak the spawn grain as u can wash off the mycelium
and roughly field capacity is not field capacity
some pictures would help though




Soaking spawn has shown to be helpful especially if they have dried out from long colonization times.
http://www.shroomery.org/forums/showflat.php/Number/17616982#17616982


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: tga8706] * 1
    #19301313 - 12/20/13 04:28 AM (10 years, 2 months ago)

lets not go there,
u add the spawn to hydrated manure/straw mix, which has more then enough water for a good few flushes

if the OP is having trouble getting the spawn to colonize the sub, then one of the steps in the whole procedure might causes this

imo soaking spawn grains is unnecessary stress
dunking grains for scelorita producers is a different thing, the grains sit in the jars for 6-9month

for cubes its 2-3 weeks


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301346 - 12/20/13 04:44 AM (10 years, 2 months ago)

Quote:

Aero said:
lets not go there,
u add the spawn to hydrated manure/straw mix, which has more then enough water for a good few flushes

if the OP is having trouble getting the spawn to colonize the sub, then one of the steps in the whole procedure might causes this

imo soaking spawn grains is unnecessary stress
dunking grains for scelorita producers is a different thing, the grains sit in the jars for 6-9month

for cubes its 2-3 weeks




Yes a masta don't whoop me masta. Didn't realize your were the self appointed thread moderator.
http://www.shroomery.org/forums/showflat.php/Number/18781503


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OfflinePussyFart
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301357 - 12/20/13 04:49 AM (10 years, 2 months ago)

Quote:

tga8706 said:
Soaking spawn has shown to be helpful especially if they have dried out from long colonization times.
http://www.shroomery.org/forums/showflat.php/Number/17616982#17616982



Quote:

Aero said:
lets not go there



God forbid we give helpful advice.....and it does not wash off the mycellium....sure "some" of it gets washed away, but it still works.

Denial of this is just ignorance, there are a lot of threads that show the benefits of dunking your spawn.

Quote:

Aero said:
imo soaking spawn grains is unnecessary stress
dunking grains for scelorita producers is a different thing, the grains sit in the jars for 6-9month
for cubes its 2-3 weeks



This is your opinion, but the facts say otherwise.


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: tga8706]
    #19301368 - 12/20/13 04:52 AM (10 years, 2 months ago)

the problem here is that the spawn doesnt colonize the sub
lets just skip the "dunk the grains" shall we?

sure Notahacker its all good with dunking, but the OP hasnt been successful with it, it didnt colonize the sub,
so if u add the spawn to the sub and it didnt colonize it what might be the problem then?


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OfflinePussyFart
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301374 - 12/20/13 04:54 AM (10 years, 2 months ago)

Quote:

Aero said:
sure Notahacker its all good with dunking, but the OP hasnt been successful with it, it didnt colonize the sub,



the dunking had nothing to do with whether or not he was successful.

Quote:

Aero said:
so if u add the spawn to the sub and it didnt colonize it what might be the problem then?



Contaminated spawn........


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301377 - 12/20/13 04:55 AM (10 years, 2 months ago)

Quote:

Aero said:
the problem here is that the spawn doesnt colonize the sub
lets just skip the "dunk the grains" shall we?

sure Notahacker its all good with dunking, but the OP hasnt been successful with it, it didnt colonize the sub,
so if u add the spawn to the sub and it didnt colonize it what might be the problem then?




Gee I don't know maybe the substrate? Call me crazy:crazy2:


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: tga8706]
    #19301387 - 12/20/13 04:57 AM (10 years, 2 months ago)

possible, yea
it even colonizes cardboard, dirt, i believe it colonizes woodchips too ( i saw a thread somewhere with that )

lets wait for the OP's response


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301402 - 12/20/13 05:06 AM (10 years, 2 months ago)

Quote:

Aero said:
possible, yea
it even colonizes cardboard, dirt, i believe it colonizes woodchips too ( i saw a thread somewhere with that )

lets wait for the OP's response




Yes poo and straw are proven to be good substrates but your assuming he prepared the substrate properly. Don't just disregard our advice cause it's not your own, the info wasn't even presented for you to disregard that's the OPs choice. Maybe the poo wasn't aged long enough or what he thought was field capacity is actually soaked or he simply lost track of time on his pasteurizing and cut it way short who knows. He didn't exactly give us alot of info to work with.


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: tga8706]
    #19301410 - 12/20/13 05:10 AM (10 years, 2 months ago)

Quote:

tga8706 said:
Quote:

Aero said:
possible, yea
it even colonizes cardboard, dirt, i believe it colonizes woodchips too ( i saw a thread somewhere with that )

lets wait for the OP's response




Yes poo and straw are proven to be good substrates but your assuming he prepared the substrate properly. Don't just disregard our advice cause it's not your own, the info wasn't even presented for you to disregard that's the OPs choice. Maybe the poo wasn't aged long enough or what he thought was field capacity is actually soaked or he simply lost track of time on his pasteurizing and cut it way short who knows. He didn't exactly give us alot of info to work with.




you are right
but as he stated he followed RR's video, i thought he went by the book..
but yes you are right there isnt much info, thats why i said to wait for the response
i used fresh hpoo worked fine btw :wink:


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: Aero]
    #19301422 - 12/20/13 05:16 AM (10 years, 2 months ago)

"i used fresh hpoo worked fine btw"

That is interesting actually although I have no desire or see reason why I would end up working with poo since I'm small scale and have plenty of other options available but cool.


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Offlinetga8706
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Re: Colonized WBS won't colonize h.manure [Re: tga8706]
    #19301436 - 12/20/13 05:21 AM (10 years, 2 months ago)

I'm not even sure which RR video he is referring to because none of them involve WBS, monotubs, or a poo/straw blend:confused:


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Offlinelisterine
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Re: Colonized WBS won't colonize h.manure [Re: cronicr]
    #19301587 - 12/20/13 06:38 AM (10 years, 2 months ago)

About the 6th day now.

Ill check temps but they are roughly right.

No, like its field capacity. One issue might be the straw not being really soaked. I put the straw in dry and i feel it didnt absorb as much water as it could have.

Should I soak the straw first then mix it into the manure next time?

Poo was fresh horse.


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Offlinelisterine
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
    #19301602 - 12/20/13 06:44 AM (10 years, 2 months ago)

what was the poo temp when u mixed in the wbs?

Roughly room temperature, maybe a bit hotter but not steaming hot.

>field capacity is not field capacity

It was field capacity, really. I doubt its that but next time ill try and make it more perfect.

>Soaking spawn has shown to be helpful especially if they have dried out

I am quite quite that soaking spawn is beneficial but ill skip that for next time. just to be safe

>Contaminated spawn........

I only used spawn that i was sure wasn't contaminated. I had some jars that had a bit of yellow water, I have a feeling that was metabolites, but I didnt use that jar just to be safe.

I think it was the substrate.


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Offlinelisterine
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Re: Colonized WBS won't colonize h.manure [Re: tga8706]
    #19301618 - 12/20/13 06:51 AM (10 years, 2 months ago)

I want to thank you for your level headed attitude and response.

>Yes poo and straw are proven to be good substrates but your assuming he prepared the substrate properly. Don't just disregard our advice cause it's not your own, the info wasn't even presented for you to disregard that's the OPs choice. Maybe the poo wasn't aged long enough or what he thought was field capacity is actually soaked or he simply lost track of time on his pasteurizing and cut it way short who knows. He didn't exactly give us alot of info to work with.

Some poo was aged for about 4 days, some a week. Two different batches mixed into one.

All of the poo has ages for about 2 weeks now, so I will use that.

>field capacity is actually soaked

Not soaked, slightly, very slightly dry. BUT... I don't think the straw held enough water content. However the myc hasn't colonized the manure either, so its not just the straw.

For next time, should i wet the straw first then add it to the manure?

>he simply lost track of time on his pasteurizing and cut it way short who knows

This is the thing. Last time i did this, i had the same problem, and i "pasteurized" for 3 hours. This time i did it for 2 hours.

Here is a very important thing. I am in a country where I can't get a meat thermometer. I read on some post (I read and found it twice, meaning it probably wasn't some shady site, but anyway) that you can put jars full of manure in water that cover it half way to 3/4s of the way. Then you bring that to a boil. Once that starts boiling, you turn off the flame and you cover the pot and leave it for 3 hours.

Thinking about it now, could that be the issue? I don't have a meat thermometer so that technique would work for me.


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OfflineAero
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Re: Colonized WBS won't colonize h.manure [Re: listerine]
    #19301622 - 12/20/13 06:53 AM (10 years, 2 months ago)

even if u over-pasteurize it, it would grow with no problem


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