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plainoldjake



Registered: 12/05/12
Posts: 60
Loc: South East, England
Last seen: 9 years, 10 months
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Bacillus troubleshooting for future grows. Advice required.
#19301295 - 12/20/13 04:20 AM (10 years, 1 month ago) |
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Hey guys, could do with a bit of help trouble shooting. I have successfully grown with rye which I spawned to a coco bulk substrate a year ago, that went fine, no trouble. Since then however, I have had a run of bad luck, my first couple of syringes I bought from someone (not a sponsor) had myc growing in them, and every time nocced up some jars within days yeast would start appearing. Got rid of those, got some spore prints off of a friend and made up my own syringes following advice found on here, they look good, everything was going well, the myc started colonizing the grains.
Roll forwards a couple of weeks and good old England decided to get cold and I have trouble heating my house, single glazed, drafty, poorly insulated rented accommodation. So I borrowed a propagator from my friend with an adjustable thermostat. Put them in there raised off of the bottom a bit and was keeping them at a steady 74f. Problem is it appears that wet spot/bacillus us growing in the jars now.
I have troubleshot my procedure and have come up with the following possible problems and was wondering if you could help me get to the bottom of this:
Jar lids, I used a cut up tyvek suit. to cover the GE holes and used silicone as a self healing injection port.
Pressure cooker not being up to standard. I think it only gets up to 13PSI, I had been running it for a bit longer to compensate but not sure that has worked.
My spore syringe being dirty, I've got no way of working with agar at the moment so can't clean them up on that.
My supply of Rye. I have just ordered 5kg from a different source to see if that helps with the problem. I soak for a minimum of 8 hours and max of 12 at room temprature and have even taken to changing the water before boiling the grains to help eliminate this problem.
Any help you could provide with this would be much appreciated as I would really like to get to the bottom of this damn thing. Starting to make me hate this lovely hobby.
Edited by plainoldjake (12/21/13 03:23 AM)
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plainoldjake



Registered: 12/05/12
Posts: 60
Loc: South East, England
Last seen: 9 years, 10 months
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: plainoldjake]
#19306258 - 12/21/13 03:24 AM (10 years, 1 month ago) |
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Bump
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: plainoldjake]
#19306525 - 12/21/13 06:00 AM (10 years, 1 month ago) |
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All that you can do is eliminate variables one by one. I'd start by getting a proper PC and move forward from there.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: grainbrain]
#19306561 - 12/21/13 06:25 AM (10 years, 1 month ago) |
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It's not your rye. 13-14 PSI for 120min or longer is fine. Make an extra jar for every step and hold it as control. One Jar that's PCed but not opened, one that's opened but not innocced, etc. (seal each of these jars with tape around the lid edge, then wait).
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indocult
Dr


Registered: 07/12/09
Posts: 1,395
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: StygianKnight]
#19306615 - 12/21/13 06:50 AM (10 years, 1 month ago) |
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How are you making your syringe, exactly? Most likely your prints are dirty or you aren't being clean in your procedures
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bluecap
mychanical



Registered: 11/10/13
Posts: 286
Last seen: 8 years, 4 months
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: indocult]
#19306632 - 12/21/13 06:56 AM (10 years, 1 month ago) |
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I bet its the pc. I would cook longer. my guess. GL.
-------------------- I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma... 
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: bluecap]
#19306795 - 12/21/13 08:00 AM (10 years, 1 month ago) |
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Make sure your venting your steam for a few minutes before putting your weight on and your grains are dry on the outside before you load.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 3 days
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: cronicr]
#19306908 - 12/21/13 08:38 AM (10 years, 1 month ago) |
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As said, let the pressure cooker vent steam for a period, but 13 psi is just fine. You had contaminated cultures or spores from the sounds of it. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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plainoldjake



Registered: 12/05/12
Posts: 60
Loc: South East, England
Last seen: 9 years, 10 months
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: RogerRabbit]
#19308307 - 12/21/13 02:41 PM (10 years, 1 month ago) |
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Thanks for all the replies, once my SFDs turn up and I can make up a few more lids and next time I load up some jars I will leave one as a control. Hopefully it is just the spores as I have hopefully found a way to get access to a flow hood and someone with some experience working with agar. I am determined to get this right.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Bacillus troubleshooting for future grows. Advice required. [Re: plainoldjake]
#19308370 - 12/21/13 03:03 PM (10 years, 1 month ago) |
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.Quote:
plainoldjake said: Hopefully it is just the spores as I have hopefully found a way to get access to a flow hood and someone with some experience working with agar. I am determined to get this right. 
You don't need a flow hood to do agar. While I find that pour agar is a little tricky in a SAB, it still can be done with success. On the other hand, no pour agar is as easy as pf tek, and an excellent way to get into it. If you can get access to a flow hood that's sweet, but if you can't you should not let that stop you from enjoying the benefits that agar offers
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